Characterization of bacterial diversity and ice-nucleating ability during different monsoon seasons over a southern tropical Indian region

Akila, M.; Priyamvada, Hema; Ravikrishna, R.; Gunthe, Sachin S.

doi:10.1016/j.atmosenv.2018.08.026

Cited by 15 publications

(12 citation statements)

References 73 publications

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“…The INP activity in Pantoea spp. has been described previously (Akila et al, ; Jimenez‐Sanchez et al, ; Kozloff et al, ; Lindow, ; Michaud et al, ; Stephanie & Waturangi, ). The plating experiments further corroborate the conjecture that biological INPs of bacterial origin are responsible for the early onset freezing temperatures in the original rainwater samples.…”

Section: Resultsmentioning

confidence: 99%

“…The INP activity in Pantoea spp. has been described previously (Akila et al, 2018;Jimenez-Sanchez et al, 2018;Kozloff et al, 1983;Lindow, 1978;Michaud et al, 2014;Stephanie & Waturangi, 2011 based on an assumed condensed water content of 0.4 g/m 3 (Petters & Wright, 2015). The gray-shaded area shows the envelope of INP observations in precipitation from Petters and Wright (Petters & Wright, 2015).…”

Section: Inp Characteristics Of Precipitation Samplesmentioning

confidence: 99%

“…The spatial variations of INP concentration range between 0 and 5 L −1 . Akila et al () report immersion mode INP activity of bacteria cultured from rainwater over India but do not report ambient INP concentrations. To our knowledge, no immersion mode INP data are available from India.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Ice‐Nucleating Particles Over Northern India

et al. 2019

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The sources and concentrations of ice‐nucleating particles (INPs) over India are not well known. Here, INP concentrations in rainwater from Northern India and a dust sample from the Thar Desert are characterized. Rainwater INP concentrations ranged between 104 and 3 × 107 L−1 water, spanning temperatures between −4 and −28 °C. During the monsoon season, INP concentrations were low and approached those in remote marine air mass. During the winter season, INPs active between −4 to −10 °C were occasionally observed. An increase in INP activity sometimes occurred after the initial onset of rain. The onset freezing temperature of samples active at warmer temperatures was shifted to colder temperature after heat treatment, suggesting that the INP activity stemmed from biological influence. Plating was used to isolate and sequence INP active bacterial strains from some of the rainwater samples, specifically strains of close taxonomic affiliation with the ice nucleating genera Pantoea. The size‐resolved ice nucleation active site density for 200–600‐nm particles of Thar Desert Dust ranged between 107 and 109 m−2 at −20 °C, values similar to dusts from other regions of the world. The data reported herein may help constrain models that seek to predict the impact of INP on the properties of mixed‐phased clouds over the Indian subcontinent.

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Section: Resultsmentioning

confidence: 99%

Section: Inp Characteristics Of Precipitation Samplesmentioning

confidence: 99%

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Characterization of Ice‐Nucleating Particles Over Northern India

et al. 2019

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“…Several studies have shown the good correlation between the concentration of fluorescent biological particles and the [INP]; however, from those studies it is highly uncertain if the good ice-nucleating abilities can be attributed to a single microorganism specie (Mason et al, 2015b;Twohy et al, 2016). Offline methods such as the one used here have been able to identify specific microorganisms such as Pseudomonas syringae, Micrococcus, Staphylococcus, Cladosporium, Penicillium, and Aspergillus from rainwater and cloud water, with some showing good ice-nucleating abilities (Amato et al, 2007(Amato et al, , 2017Delort et al, 2010;Failor et al, 2017;Stopelli et al, 2017;Akila et al, 2018).…”

Section: Identification Of the Potential Inp Sourcesmentioning

confidence: 94%

Ice-nucleating particles in a coastal tropical site

et al. 2019

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Abstract. Atmospheric aerosol particles that can nucleate ice are referred to as ice-nucleating particles (INPs). Recent studies have confirmed that aerosol particles emitted by the oceans can act as INPs. This very relevant information can be included in climate and weather models to predict the formation of ice in clouds, given that most of them do not consider oceans as a source of INPs. Very few studies that sample INPs have been carried out in tropical latitudes, and there is a need to evaluate their availability to understand the potential role that marine aerosol may play in the hydrological cycle of tropical regions. This study presents results from the first measurements obtained during a field campaign conducted in the tropical village of Sisal, located on the coast of the Gulf of Mexico of the Yucatan Peninsula in Mexico in January–February 2017, and one of the few data sets currently available at such latitudes (i.e., 21∘ N). Aerosol particles sampled in Sisal are shown to be very efficient INPs in the immersion freezing mode, with onset freezing temperatures in some cases as high as −3 ∘C, similarly to the onset temperature from Pseudomonas syringae. The results show that the INP concentration in Sisal was higher than at other locations sampled with the same type of INP counter. Air masses arriving in Sisal after the passage of cold fronts have surprisingly higher INP concentrations than the campaign average, despite their lower total aerosol concentration. The high concentrations of INPs at warmer ice nucleation temperatures (T>-15 ∘C) and the supermicron size of the INPs suggest that biological particles may have been a significant contributor to the INP population in Sisal during this study. However, our observations also suggest that at temperatures ranging between −20 and −30 ∘C mineral dust particles are the likely source of the measured INPs.

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“…The IN activity of birch tree extracts stems from macromolecules or aggregates of macromolecules which involve polysaccharides (Pummer et al, 2012) and proteins (Tong et al, 2015;Felgitsch et al, 2018) that may coaggregate. Similarly, the exudate material acting as INPs in marine aerosol (Wilson et al, 2015;Ladino et al, 2016) was found to contain polysaccharidic and proteinaceous compounds (Aller et al, 2017). Finally, ice-nucleating proteins expressed by Pseudomonas exhibit a repetition unit containing threonine amino acids with hydroxyl functional groups that are able to template ice.…”

Section: Introductionmentioning

confidence: 99%

Protein aggregates nucleate ice: the example of apoferritin

et al. 2020

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Abstract. Biological material has gained increasing attention recently as a source of ice-nucleating particles that may account for cloud glaciation at moderate supercooling. While the ice-nucleation (IN) ability of some bacteria can be related to membrane-bound proteins with epitaxial fit to ice, little is known about the IN-active entities present in biological material in general. To elucidate the potential of proteins and viruses to contribute to the IN activity of biological material, we performed bulk freezing experiments with the newly developed drop freezing assay DRoplet Ice Nuclei Counter Zurich (DRINCZ), which allows the simultaneous cooling of 96 sample aliquots in a chilled ethanol bath. We performed a screening of common proteins, namely the iron storage protein ferritin and its iron-free counterpart apoferritin, the milk protein casein, the egg protein ovalbumin, two hydrophobins, and a yeast ice-binding protein, all of which revealed IN activity with active site densities > 0.1 mg−1 at −10 ∘C. The tobacco mosaic virus, a plant virus based on helically assembled proteins, also proved to be IN active with active site densities increasing from 100 mg−1 at −14 ∘C to 10 000 mg−1 at −20 ∘C. Among the screened proteins, the IN activity of horse spleen ferritin and apoferritin, which form cages of 24 co-assembled protein subunits, proved to be outstanding with active site densities > 10 mg−1 at −5 ∘C. Investigation of the pH dependence and heat resistance of the apoferritin sample confirmed the proteinaceous nature of its IN-active entities but excluded the correctly folded cage monomer as the IN-active species. A dilution series of apoferritin in water revealed two distinct freezing ranges, an upper one from −4 to −11 ∘C and a lower one from −11 to −21 ∘C. Dynamic light scattering measurements related the upper freezing range to ice-nucleating sites residing on aggregates and the lower freezing range to sites located on misfolded cage monomers or oligomers. The sites proved to persist during several freeze–thaw cycles performed with the same sample aliquots. Based on these results, IN activity seems to be a common feature of diverse proteins, irrespective of their function, but arising only rarely, most probably through defective folding or aggregation to structures that are IN active.

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Characterization of bacterial diversity and ice-nucleating ability during different monsoon seasons over a southern tropical Indian region

Cited by 15 publications

References 73 publications

Characterization of Ice‐Nucleating Particles Over Northern India

Characterization of Ice‐Nucleating Particles Over Northern India

Ice-nucleating particles in a coastal tropical site

Protein aggregates nucleate ice: the example of apoferritin

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