Characterization of bioactive compounds in defatted hempseed (<i>Cannabis sativa</i>L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes

Martinez, Julio Rea; Paz, Sergio Montserrat‐de la; Puerta, R. de la; García-Giménez, M.D.; Fernández-Arche, M.A.

doi:10.1039/d0fo00066c

Cited by 32 publications

(32 citation statements)

References 37 publications

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“…Hemp inflorescences biosynthesise quite unique health beneficial phytocannabinoids, while hemp seeds are an excellent source of high-value oil, proteins, and valuable micronutrients [6], including health beneficial bioactive compounds [7,8]. For instance, Pihlanto et al [9] reported 5.88-10.63 mg total phenolic content (expressed in mg of gallic acid equivalents) per gram of dry defatted hemp seed flour, which was also confirmed by Rea et al [10].…”

Section: Introductionmentioning

confidence: 72%

“…Sweet grass extracts were reported as being very strong antioxidants in rapeseed oil [30], and later 5,8-dihydroxycoumarin and its glycoside were identified as the main radical scavengers and new natural compounds [12]. Recently, Martinez et al [8] reported that the fractions isolated with a hydroethanolic mixture and ethyl acetate from the defatted press-cake with hexane hempseeds contained N-transcaffeoyltyramine as one of the main bioactives and reduced the inflammatory competence of lipopolysaccharide-treated human primary monocytes.…”

Section: Characterisation Of Ingredientsmentioning

confidence: 99%

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Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties

Kerner

Jõudu

Tänavots

et al. 2021

Foods

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There is an increasing tendency toward the application of plant origin ingredients in meat products. This study evaluates the physicochemical properties and oxidative stability of pork burger patties produced with the addition of dried raw and defatted by supercritical CO2 extraction hemp seed press-cake as protein-rich ingredients (1.5–2.0%) and sweet grass ethanolic extract (0.5%) as a strong natural antioxidant. The main aim of using such a combination was to assess the possibility of mitigating the negative effects of hemp seed press-cake, containing approx. 10% of highly unsaturated oil, on the oxidation of meat products. The patties were compared with the control sample (without additives) during storage on days 0, 4, 8, 15, and 21 at 4 °C in modified atmosphere conditions. Plant ingredients reduced the lightness of pork patties, while their effects on other physicochemical characteristics were insignificant. The patties with fully defatted hemp seed flour showed the lowest grilling losses. Based on the measurement of thiobarbituric acid reactive substances, raw hemp seed press-cake increased the oxidative rate of pork patties; however, remarkably, the addition of sweet grass extract completely inhibited oxidation during the whole period of storage. The sensory characteristics of the products were acceptable; however, the patties with sweet grass extract received lower evaluation scores.

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Section: Introductionmentioning

confidence: 72%

Section: Characterisation Of Ingredientsmentioning

confidence: 99%

Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties

Kerner

Jõudu

Tänavots

et al. 2021

Foods

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“…Studies have shown that caffeoyltyramine is the major compound in the fraction with a concentration above 6.36 mg/g extract. 17 …”

Section: Resultsmentioning

confidence: 99%

“…These compounds derive from the products of conjugation between phenolic acids and arylmonoamines, such as tyramine and octopamine, which are present in hempseed in large proportions 19 , 20 and in high quantities. 17 Hempseed can be a source of potential candidates with application in neurodegenerative diseases. It has also been reported that N -methylated tyramine derivatives of Ginkgo biloba can cross the BBB.…”

Section: Resultsmentioning

confidence: 99%

Dual Role of Phenyl Amides from Hempseed on BACE 1, PPARγ, and PGC-1α in N2a-APP Cells

et al. 2021

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In Alzheimer’s disease (AD) the accumulation of amyloid β (Aβ) plaques in the brain leads to neuroinflammation, neuronal cell dysfunction, and progressive memory loss. Therefore, blocking the formation of Aβ plaques has emerged as one of the most promising strategies to develop AD treatments. Hempseed is widely used as a food, and recently its compounds have shown beneficial effects on neuroinflammation. The objective of this study was to investigate whether a fraction rich in phenyl amide compounds, N - trans -caffeoyltyramine (CAFT) and N - trans -coumaroyltyramine (CUMT), can affect gene expression: β-site amyloid-precursor-protein-cleaving enzyme 1 (BACE 1), peroxisome proliferator-activated receptor gamma (PPAR γ), and PPARγ-coactivator-1α (PGC-1α) in N2a-APP cells. The mRNA levels were measured using RT-qPCR. The ethyl acetate fraction and CAFT were found to reduce BACE1 gene expression and are promissory PPARγ and PGC-1α natural agonists. The results show that hempseed compounds can inhibit the expression of BACE 1, which is involved in the accumulation of Aβ plaques and positively affect transcription factors involved in complex and diverse biological functions.

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“…In terms of phytoconstituents, multiple studies describing diverse analytical methods for the determination of cannabinoids and terpenes in cannabis matrices have been recently reported [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Furthermore, SMPRs for the quantitation of cannabinoids in cannabis concentrates have been published [ 38 ], and several literature reviews outlining the most up‐to‐date information in terpene and potency analysis are also available [ 7 , 8 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the California list of pesticides, mycotoxins, and cannabinoids in chocolate using liquid chromatography and low‐pressure gas chromatography‐based platforms

Reyes‐Garcés

Myers

2021

J of Separation Science

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Cannabis legalization has led to the development of a variety of cannabis-infused products with edibles being one of the most popular. The state of California has implemented comprehensive cannabis testing regulations requiring the analysis of cannabinoids (potency) and contaminants, such as pesticides and mycotoxins, in any type of cannabis good. In this work, we propose an analytical workflow for the quantification of the California list of pesticides and mycotoxins, as well as six cannabinoids, in chocolate, using 3 mL of solvent for the extraction. For the analysis of pesticides and mycotoxins, clean-up steps employing a C18 solidphase extraction cartridge and dispersive solid-phase extraction sorbents were implemented. Gas chromatography amenable pesticides were analyzed using low-pressure gas chromatography coupled to tandem mass spectrometry which allowed for a total method run of 12 min. Both liquid chromatography and gas chromatography instrumental methods had the same analysis time, ensuring satisfactory sample throughput. For the determination of cannabinoids, a dilution of the original organic extract collected for pesticides and mycotoxins analysis (and prior to any clean-up step) was used. Excellent results in terms of analytical figures of merit were obtained for all target analytes.

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Characterization of bioactive compounds in defatted hempseed (Cannabis sativaL.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes

Abstract: Anti-inflammatory effects on human monocytes by phenolic amides present in defatted hempseed fractions (Cannabis sativa L.).

Cited by 32 publications

References 37 publications

Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties

Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties

Dual Role of Phenyl Amides from Hempseed on BACE 1, PPARγ, and PGC-1α in N2a-APP Cells

Analysis of the California list of pesticides, mycotoxins, and cannabinoids in chocolate using liquid chromatography and low‐pressure gas chromatography‐based platforms

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