Characterization of bioactive RGD peptide immobilized onto poly(acrylic acid) thin films by plasma polymerization

Seo, Hyun; Ko, Yeong-Mu; Shim, Jae Won; Lim, Yun Kyong; Kook, Joong-Ki; Cho, Dong-Lyun; Kim, Byung Hoon

doi:10.1016/j.apsusc.2010.07.040

Cited by 41 publications

(14 citation statements)

References 25 publications

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“…For the PolyHIPEs investigated here, relative stiffness alone did not appear to induce differentiation as significantly higher ALP activity only occurs in conjunction with pdAAc. With regards to the effect of acrylic acid on osteogenic differentiation, conclusive evidence for a relationship is yet to be seen as there is evidence in the literature indicating stimulatory ( Mattioli-Belmonte et al, 2005 ) as well as no ( Seo et al, 2010 ) effects. It has been shown that plasma deposited acrylic acid does not diminish the cells’ ability to perceive differences in substrate stiffness when comparing the osteogenic response of MSCs to varied substrate stiffness ( Colley et al, 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering

Owen

Sherborne

Paterson

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

108

122

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Polymerised High Internal Phase Emulsions (PolyHIPEs) are manufactured via emulsion templating and exhibit a highly interconnected microporosity. These materials are commonly used as thin membranes for 3D cell culture. This study uses emulsion templating in combination with microstereolithography to fabricate PolyHIPE scaffolds with a tightly controlled and reproducible architecture. This combination of methods produces hierarchical structures, where the microstructural properties can be independently controlled from the scaffold macrostructure. PolyHIPEs were fabricated with varying ratios of two acrylate monomers (2-ethylhexyl acrylate (EHA) and isobornyl acrylate (IBOA)) and varying nominal porosity to tune mechanical properties. Young’s modulus, ultimate tensile stress (UTS) and elongation at failure were determined for twenty EHA/IBOA compositions. Moduli ranged from 63.01±9.13 to 0.36±0.04 MPa, UTS from 2.03±0.33 to 0.11±0.01 MPa and failure strain from 21.86±2.87% to 2.60±0.61%. Selected compositions were fabricated into macro-porous woodpile structures, plasma treated with air or acrylic acid and seeded with human embryonic stem-cell derived mesenchymal progenitor cells (hES-MPs). Confocal and two-photon microscopy confirmed cell proliferation and penetration into the micro- and macro-porous architecture. The scaffolds supported osteogenic differentiation of mesenchymal cells and interestingly, the stiffest IBOA-based scaffolds that were plasma treated with acrylic acid promoted osteogenesis more strongly than the other scaffolds.

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Section: Discussionmentioning

confidence: 99%

Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering

Owen

Sherborne

Paterson

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

108

122

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“…Moreover, it is a versatile technique to prepare a homogeneous and pinhole free film with good surface coverage [8]. Precursors like allylamine and acryl acid have been employed to form plasma polymerized films with the desirable functional groups on metallic materials [9,10]. But, so far most researches focus on the biocompatibility of the polymer films and the corrosion protection offered by the coatings has seldom been reported.…”

Section: Introductionmentioning

confidence: 99%

In vitro corrosion inhibition on biomedical shape memory alloy by plasma-polymerized allylamine film

Ren-ping

et al. 2012

Materials Letters

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“…Immobilization of RGD on biomaterials including PU has been accomplished only on thin films and limited thickness scaffolds (1–3 mm), or electro‐spun fibre meshes (Guler, Silva, & Sezai Sarac, ; Lin et al, ; Seo et al, ) due to the inability of cells to infiltrate scaffold cores, directly related to scaffold thickness (Lamprecht et al, ; Lopez et al, ; Tocchio et al, ; Yoon, Song, Lee, & Park, ). Achieving homogeneous RGD distribution is crucial, because uniform cell penetration within a significant thickness scaffold still represents a challenge.…”

Section: Introductionmentioning

confidence: 99%

RGD‐functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation

Tahlawi

Klontzas

Allenby

et al. 2018

J Tissue Eng Regen Med

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Biomimetic materials are essential for the production of clinically relevant bone grafts for bone tissue engineering applications. Their ability to modulate stem cell proliferation and differentiation can be used to harness the regenerative potential of those cells and optimize the efficiency of engineered bone grafts. The arginyl-glycylaspartic acid (RGD) peptide has been recognized as the adhesion motif of various extracellular matrix proteins and can affect stem cell behaviour in biomaterials.Attempts to functionalize biomaterials with RGD have been limited to a maximum of 1-to 3-mm thickness scaffolds, overlooking the issue of core infiltration that represents a major hurdle in developing real thickness scaffolds. Herein, we present the cross-linking of RGD on the surface of "real thickness" (5 × 5 × 5 mm) porous polyurethane scaffolds (PU-RGD), to be used for the expansion and osteogenic differentiation of umbilical cord blood mesenchymal stem cells (UCB MSCs). RGD-functionalized scaffolds increased initial cell adhesion (1.5-fold to twofold) and achieved a 3.4-fold increase in cell numbers at the end of culture compared with a 1.5-fold increase in non-functionalized controls. Homogenous cell infiltration to the scaffold core was observed in the PU-RGD scaffolds. Importantly, PU-RGD scaffolds were able to enhance the osteogenic differentiation of UCB MSCs. Osteogenic gene and protein expression increased in scaffolds functionalized with 100 μg/ml RGD. Higher RGD concentrations (200 μg/ml) were less efficient in stimulating osteogenic differentiation. We conclude that robust RGD tethering to 3D PU "real thickness" scaffolds is possible and that it promotes core infiltration, expansion, and osteogenic differentiation of UCB MSCs for the purposes of bone regeneration.

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Characterization of bioactive RGD peptide immobilized onto poly(acrylic acid) thin films by plasma polymerization

Cited by 41 publications

References 25 publications

Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering

Emulsion templated scaffolds with tunable mechanical properties for bone tissue engineering

In vitro corrosion inhibition on biomedical shape memory alloy by plasma-polymerized allylamine film

RGD‐functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation

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