Characterization of blood components separated from donated whole blood after an overnight holding at room temperature with the buffy coat method

Abstract

These data suggest that blood components (RBCs, pooled PCs, and FP) separated from WB that has been stored overnight at room temperature by the BC method are of acceptable quality.

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…Swirling, which is the ability of functional discoid platelets to align to a current in the storage medium, was not different. Others found a higher CD62P expression and annexin A5 binding at onset for platelet concentrates from 8-vs 24-hour-held whole blood, but this difference disappeared on day 3 [36]. In this study, platelet yield was 14% lower in the 8-hour hold group.…”

“…Our group found a decline in factor III:C from 1.22 ± 0.31 IU/mL when processed within 2 hours of collection vs 1.05 ± 0.25 IU/mL when processed after 16 to 20 hours (14% lower; P b .001) [14]; others found no difference in factor V and a 33% or 12% lower value for factor VIII, in a study that reported the results of 2 processing sites [19]. An evaluation of 9 clotting factors showed the same pattern as others [36]. Finally, a multisite study of the BEST Collaborative [52] demonstrated a 6% lower protein C and a 14% lower protein S, but no change in antithrombin for whole blood held for 24 to 26 vs less than 8 hours in a paired study; factor VIII was 23% lower.…”

“…No effect (shown for PRP-derived platelets only) Platelet concentration 0-30% higher [4,19,30,32] pH, lactate pH lower, lactate higher, but disappears during storage [6,13,29,31,34,36] HSR HSR initially lower, but disappears during storage [13,31,34,36] Other PLT quality measures…”

“…Minor effects that disappear during storage [13,31,34,36] Leukoreduction Less efficient with older types of filters, but residual white cells within specifications. For currently marketed filters no difference [34], Table 1 Risk of bacterial outgrowth…”

Evaluation of Random Donor Platelets Produced from Buffy Coat Stored for 24 h at Ambient Temperature: Should This be Implemented in India?

“…In our study we found that findings were not statistically significant [11]. Another similar study done by Lu et al [12] revealed that the platelet yield in PCs prepared from an overnight-hold WB sample was significantly higher, while CD62P expression and annexin V binding were lower (p \ 0.05). These findings were comparable with our results.…”