Characterization of cDNAs Encoding Two Distinct Miraculin-like Proteins and Stress-related Modulation of the Corresponding mRNAs in Citrus jambhiri Lush

Tsukuda, Shintaro; Gomi, Kenji; Yamamoto, Hiroyuki; Akimitsu, Kazuya

doi:10.1007/s11103-005-2941-4

Cited by 77 publications

(66 citation statements)

References 46 publications

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“…In Citrus jambhiri, two distinct transcripts of miraculin-like proteins accumulated to higher levels in leaves after wounding, inoculation with conidia of Alternaria alternata, or treatment with methyl jasmonate vapors (Tsukuda et al, 2006). Stress-inducible genes such as pathogenesis-related class Chitinase (PR3), PR10 (SGR1), Peroxidase prxpr1 (PR9) and a germin-like protein (PR16) were also observed as potentially overexpressed transcripts in CTV inoculated plants.…”

Section: Defense-related Genesmentioning

confidence: 99%

Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization

et al. 2007

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Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs (Tentative Consensi sequences) were obtained using both cDNA libraries in a single clusterization procedure. By the in silico hybridization approach, 289 TCs were identified as differentially expressed in the two libraries. A total of 121 TCs were found to be overexpressed in plants infected with CTV and were grouped in 12 primary functional categories. The majority of them were associated with metabolism and defense response. Some others were related to lignin, ethylene biosynthesis and PR proteins. In general, the differentially expressed transcripts seem to be somehow involved in secondary plant response to CTV infection.

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Section: Defense-related Genesmentioning

confidence: 99%

Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization

et al. 2007

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“…El superunigen CcMLP1 presenta a su vez una homología del 97% con la secuencia de aminoácidos de RLemMLP1, de Citrus jambhiri. (Tsukuda et al, 2006). CcMLP5 y CcMLP6 presentan una homología de 98 y 87%, respectivamente, con la secuencia de RLemMLP2 de Citrus jambhiri.…”

Section: Miraculinas De Cítricos Bajo Condiciones De Estrés Hídricounclassified

“…CcMLP5 y CcMLP6 presentan una homología de 98 y 87%, respectivamente, con la secuencia de RLemMLP2 de Citrus jambhiri. (Tsukuda et al, 2006). La secuencia del superunigen CcMLP4 solo presenta una homología del 54% con la secuencia de RLemMLP2 de Citrus jambhiri.…”

Section: Miraculinas De Cítricos Bajo Condiciones De Estrés Hídricounclassified

“…Los estudios realizados con miraculinas en cítricos se han llevado a cabo para determinar su relación con la defensa de la planta contra estreses bióticos (Shatters et al, 2004;Tsukuda et al, 2006). Según nuestros resultados podemos ver que puede producirse el aumento de los niveles de transcrito de algunas de ellas bajo condiciones de estrés abiótico, no solo en rotura, que se podría relacionar con la propia rotura que sufre la planta ante un estrés biótico sino también bajo condiciones de estrés hídrico.…”

Section: Expresión De Las Miraculinas De Cítricosunclassified

“…Los dos cDNAs de Citrus jambhiri, RlemMLP1 y RlemMLP2, se han obtenido a partir de una biblioteca de hojas de plantas inoculadas con el hongo Alternaria alternata (Gomi et al, 2003). Un estudio mas detallado de ambos genes (Tsukuda et al, 2006), determinó la acumulación del trascrito en frutos pero no en hojas de plantas no infectadas. Tras inocular las hojas con esporas de dos cepas de A. alternata no patogénicas el hongo indujo la expresión de ambos genes en hojas.…”

Section: Introductionunclassified

See 2 more Smart Citations

Respuesta transcripcional al estrés hídrico en mandarino. Estudio genómico-funcional con micromatrices de cDNA

Romeu¹

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Bioinsecticidal activity of Murraya koenigii miraculin‐like protein against Helicoverpa armigera and Spodoptera litura

Gahloth¹,

Shukla²,

Birah³

et al. 2011

Arch Insect Biochem Physiol

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Miraculin-like proteins, belonging to the Kunitz superfamily, are natural plant defense agents against pests and predators, and therefore are potential biopesticides for incorporation into pest-resistant crops. Here, a miraculin-like protein from Murraya koenigii was assessed for its in vitro and in vivo effects against two polyphagous lepidopteran insect pests, Helicoverpa armigera and Spodoptera litura. M. koenigii miraculin-like protein (MKMLP) inhibited the trypsin-like activity and total protease activity of H. armigera gut proteinases (HGP) by 78.5 and 40%, respectively, and S.litura gut proteinases (SGP) by 81 and 48%, respectively. The inhibitor was stable and actively inhibited the proteolysis of both HGP and SGP enzymes for up to 72 h. Incorporation of MKMLP into artificial diet adversely affected the growth and development of pests in a dose-dependent manner. After 10 days of feeding on diets containing 200 µM MKMLP, larval weight was reduced to 69 and 44.8% and larval mortality was increased to 40 and 43.3% for H. armigera and S litura, respectively. The LC(50) of MKMLP was 0.34 and 0.22% of the diet for H.armigera and S. litura, respectively. These results demonstrate the efficacy of MKMLP as a potential plant defense agent against H. armigera and S. litura.

show abstract

Characterization of cDNAs Encoding Two Distinct Miraculin-like Proteins and Stress-related Modulation of the Corresponding mRNAs in Citrus jambhiri Lush

Cited by 77 publications

References 46 publications

Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization

Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization

Respuesta transcripcional al estrés hídrico en mandarino. Estudio genómico-funcional con micromatrices de cDNA

Bioinsecticidal activity of Murraya koenigii miraculin‐like protein against Helicoverpa armigera and Spodoptera litura

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