2018
DOI: 10.1080/21688370.2017.1405774
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Characterization of cell-cell junction changes associated with the formation of a strong endothelial barrier

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Cited by 26 publications
(18 citation statements)
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“…This was not surprising given the significant evidence that these supplements improve the barrier phenotype in ECs [24,25]. Of specific relevance, one study reported increased TEER and localization of ZO-1 and VE-cadherin, showing a more linear morphology (assessed qualitatively) in HUVECs treated with the same concentrations of cAMP supplements for 1 day, supporting our results in this study [31]. Interestingly, increasing culture time in the presence of cAMP did not increase junction coverage for ZO-1 or VE-cadherin, and required longer cAMP treatment to reach similar presentation values observed in shorter experiments (FBN results presented in Additional file 1: Figure S16).…”
Section: Discussionsupporting
confidence: 89%
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“…This was not surprising given the significant evidence that these supplements improve the barrier phenotype in ECs [24,25]. Of specific relevance, one study reported increased TEER and localization of ZO-1 and VE-cadherin, showing a more linear morphology (assessed qualitatively) in HUVECs treated with the same concentrations of cAMP supplements for 1 day, supporting our results in this study [31]. Interestingly, increasing culture time in the presence of cAMP did not increase junction coverage for ZO-1 or VE-cadherin, and required longer cAMP treatment to reach similar presentation values observed in shorter experiments (FBN results presented in Additional file 1: Figure S16).…”
Section: Discussionsupporting
confidence: 89%
“…Samples were treated with medium containing cAMP supplements: 250 μM 8-CPT-cAMP (Abcam, ab120424) and 17.5 μM RO-20-1724 (Tocris Bioscience, 0415), for 1, 3, or 6 days, or control HBMEC medium. These supplements are routinely used in EC culture to improve junction localization and barrier properties [4,[30][31][32][33]. For all experiments, the medium was first changed the day after cell seeding, then again on Days 3, 4, and 6 for the respective culture lengths.…”
Section: Substrate Coating and Experimental Conditionsmentioning
confidence: 99%
“…Many studies have shown that poloxamers are capable of sealing the compromised cell membrane. For example, the FDA-approved poloxamers P188 was demonstrated to reconstitute the membrane in BBB 30,31 and down-regulated the secretion of matrix metalloproteinases (MMP) 32,33 by likely modulating the TNF-α pathway 34 . In this study, we cultured a monolayer of brain endothelial cells on a well-characterized synthetic membrane and quantitatively determined changes in the permeability and disorganized tight junctions in response to the blast-induced microcavitation.…”
mentioning
confidence: 99%
“…Oxidative stress is a primary mediator of neurologic injury following ischemic stroke [ 49 ] and entails various mechanisms of the “ischemic cascade,” which leads to cell death and indicates that oxidative stress may become a potential therapeutic target of ischemic stroke. Cell junctions may play key roles in regulation of BBB stability and permeability to paracellular compounds [ 50 ]. It was thought that several QKL targets are expressed in cell junctions indicating a BBB modulating effect.…”
Section: Discussionmentioning
confidence: 99%