Characterization of Cell Cycle Events in Synchronized Cultures of Anacystis nidulans

Asato, Yukio

doi:10.1099/00221287-130-10-2535

Cited by 6 publications

(34 citation statements)

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“…The lack of synchronous cell division upon release of dark-blocked Synechococcus cells into the light contrasts with some of the results of others (2,3,18,20), but the experimental conditions were not identical in these cases.…”

Section: Resultsmentioning

confidence: 38%

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Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301

1990

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Flow cytometry was used to examine cell cycle regulation in Synechococcus sp. strain PCC 6301 under a variety of growth conditions. The DNA frequency distributions of exponentially growing and dark-blocked populations confirmed that this cyanobacterium contains multiple chromosome copies even at very slow growth rates. Furthermore, the presence of major peaks corresponding to other than 2" chromosome copies strongly suggests that DNA replication is initiated asynchronously. Although this suggestion is at odds with the standard formulation of the procaryotic cell cycle model, it is similar to recent observations of asynchrony in Escherichia coli replication mutants.

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Section: Resultsmentioning

confidence: 38%

“…3G). Furthermore, at all but the highest growth rate examined, Light Intensity (,uE m-2 s-i) 4 the balanced-growth DNA frequency distributions contained distinct peaks (Fig. 3C to G), indicative of gaps in DNA synthesis.…”

Section: Resultsmentioning

confidence: 99%

Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301

1990

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“…During the first 8 h after the dark to light shift, protein, RNA and DNA synthesis are sequentially initiated followed by cell septum formation, which is usually completed after 8 h (Asato, 1983). Subsequent cell cycles manifest the same sequences of cell events (Asato, 1979). Synchronized DNA synthesis within the synchronized cell cycle has been demonstrated in A .…”

Section: Introductionmentioning

confidence: 82%

“…nidulans by others (Herdman et al, 1970;Herdman & Carr, 1971 ;Delaney & Carr, 1975 ;Gleason & Ooka, 1978;Bagi et al, 1979). Phospholipid (Asato, 1979) and cell wall synthesis (Asato, 1984) and peak rates of O2 evolution (Csatorday & Horvath, 1977) also occur at specific times within the cell cycle. It was concluded that the periods of macromolecular synthesis are major events in the cell developmental cycle of A .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Cell Cycle Events in the Dark in Anacystis nidulans

Marino¹,

Asato²

1986

Microbiology

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~~Anucystis niduluns (Synechococcus PCC 6301) is an obligate phototrophic cyanobacterium. When light-grown cultures of Anucystis are transferred to the dark, the on going cell cycles are aborted. To characterize the fates of cell cycle events in the dark, synchronized cultures of A . nidulans, taken at various phases of growth, were placed in the dark and the macromolecular contents and cell numbers were determined. Cell number did not increase in any culture in the dark. Protein and RNA contents remained the same. However, cultures in the last hour of their respective synthesis periods showed detectable increases in protein and RNA contents. In cultures in the early stages of DNA synthesis, no sustained increase in DNA was observed, indicating that DNA replication was not completed in the dark by these cultures. However, incorporation of 32P in the DNA fraction in the dark suggested that DNA replication was completed for cultures in the last stages of DNA synthesis. These results suggest that macromolecular synthesis and cell septum formation were curtailed (with the exceptions indicated above) and further progress in the cell cycle stopped in the dark.

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Toward an understanding of cell growth and the cell division cycle of unicellular photoautotrophic cyanobacteria

Asato¹

2003

Cellular and Molecular Life Sciences (CMLS)

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The cell division cycle of Synechococcus sp. strain PCC 6301 in light is characterized by the sequential and orderly appearance of macromolecular synthesis periods. In the dark, macromolecular synthesis and cell division are severely curtailed. When dark-incubated cultures are reexposed to light, a new cell cycle is initiated. The pattern of the cell events displayed by Synechococcus in light and the absence of sustained growth in dark incubation conditions suggests that light-activated regulatory molecules control macromolecular synthesis and the cell division cycle. For example, ribosomal RNA synthesis is stimulated by a light-activated DNA binding factor in light but not in the dark. Light/dark conditions induce cell synchrony in Prochlorococcus. Distinct G1, S and G2 phases characterize cell cycles of marine Synechococcus and Prochlorococcus. Cell division in Synechococcus elongatus PCC 7942 and marine Synechococcus is controlled by circadian oscillators.

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Characterization of Cell Cycle Events in Synchronized Cultures of Anacystis nidulans

Cited by 6 publications

References 0 publications

Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301

Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301

Characterization of Cell Cycle Events in the Dark in Anacystis nidulans

Toward an understanding of cell growth and the cell division cycle of unicellular photoautotrophic cyanobacteria

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