Characterization of cell lines derived from the southern armyworm, Spodoptera eridania

Zhang, Hongwei; Li, Yaofa; Reall, Tamra; Xu, Yiyun; Goodman, Cynthia L.; Saathoff, Stephen G.; Ringbauer, Joseph A.; Stanley, David

doi:10.1007/s11626-018-0300-4

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…We used three S. frugiperda cell lines, including two newly established lines that were generated using the protocols and materials described earlier (Reall et al, 2019; Zhang et al, 2018): BCIRL‐SfNS‐0518B‐YL derived from the S. frugiperda nervous system (SfNS) and BCIRL‐Sf4FB‐0614‐SGS derived from S. frugiperda fat body (SfFB). Both lines were maintained in CLG#2 medium, a 1:1 mixture of Leibovitz's L15 medium (Caisson Labs) and EX‐CELL 420 (Sigma‐Aldrich, Inc.).…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Wang

Goodman

Ringbauer

et al. 2021

Arch Insect Biochem Physiol

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Animals maintain homeostasis of cell numbers, constantly creating new cells and eliminating others. Programmed cell death, apoptosis, is a mechanism of cell elimination and it acts in many aspects of animal biology. Drawing on the biomedical background, several signals launch the apoptosis mechanisms, including prostaglandins (PGs). Based on this information, we posed the hypothesis that PGs similarly induce apoptosis in insect cell lines. We used three Spodoptera frugiperda cell lines, including two newly established, BCIRL-SfNS-0518B-YL derived from the central nervous system and BCIRL-Sf4FB-0614-SGS derived from fat body, and the commercially available Sf9 cells. Using a kinetic apoptosis kit, we found treating SfNS cells for 18 h with 15 or 20 μM PGA 2 led to decreases in cell numbers, coupled with increased numbers of apoptotic and dead cells. Similar exposures to 10 μM PGA 2 (24 h) led to substantial increases in apoptotic cells, confirmed by a terminal deoxynucleotidyl transferase dUTP nick end labeling assay on a flow cytometer. The influence of PGA 2 treatments increased with dosage, as we recorded about 20% apoptosis at 24 h post-PGA 2 treatments (10 μM) and about 34% apoptosis at 24 h post-30 μM treatments. PGA 2 treatments led to 10-to 30-fold increases in messenger RNAs (mRNAs) encoding apoptosis-specific caspases-1, −2, −3, and −5 at 12 h and 40-to 60-fold increases in mRNAs encoding

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Section: Methodsmentioning

confidence: 99%

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Wang

Goodman

Ringbauer

et al. 2021

Arch Insect Biochem Physiol

Self Cite

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“…For example, Watanabe [ 11 ] built novel cell lines originating from the fat bodies of six lepidopteran insects. Zhang [ 12 ] reported the creation of cell lines from the fat body, nervous system and reproductive tissue of Spodoptera eridania larvae. Zhou [ 13 ] reported three established cell lines, DvWL1, 2 and 3, which were first derived from Diabrotica virgifera virgifera .…”

Section: Establishment Of Insect Cell Linesmentioning

confidence: 99%

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Huang

et al. 2023

Insects

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During the past decades, research on insect cell culture has grown tremendously. Thousands of lines have been established from different species of insect orders, originating from several tissue sources. These cell lines have often been employed in insect science research. In particular, they have played important roles in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review intends to first briefly summarize the progression of insect cell line establishment. Then, several recent studies based on insect cell lines coupled with advanced technologies are introduced. These investigations revealed that insect cell lines can be exploited as novel models with unique advantages such as increased efficiency and reduced cost compared with traditional insecticide research. Most notably, the insect cell line-based models provide a global and in-depth perspective to study the toxicology mechanisms of insecticides. However, challenges and limitations still exist, especially in the connection between in vitro activity and in vivo effectiveness. Despite all this, recent advances have suggested that insect cell line-based models promote the progress and sensible application of insecticides, which benefits pest management.

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“…Heliothine cell lines, including from embryonic and larval ventral nerve cords, have been developed 43 . Recently, four southern armyworm Spodoptera eridania cell lines have been developed, some of which are hypothesized to be neuronal 44 . A 2006 review of the insect neuronal cell culture field provides a foundation for further manipulation of insect cells, including for potential use in insecticide discovery 45 .…”

Section: Lab‐on‐a‐chip or Organ‐on‐a‐chip Technologies For Insecticide Screening Or Mode Of Action Studiesmentioning

confidence: 99%

Pharmaceutical technologies with potential application to insecticide discovery^†

Wing¹

2020

Pest Management Science

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Novel neuroactive insecticides are discovered/registered differently, have a lower value in use, and exert their physiological actions in manners distinct from neuroactive pharmaceuticals, but there are clear similarities in their biochemical modes of action. Insecticides are generally discovered using whole pest insect screens, and this eases difficulties in ‘translational science’ from laboratory to field, as opposed to pharmaceutical translation from biochemical or cell‐based targets to animal models to human clinical trials to registered drug. This paper examines recent trends in pharmaceutical science and identifies some technologies which may represent complementary approaches to insecticide discovery screening and mode of action determination beyond the sound processes in common practice today. Examples will be drawn from nanoparticle delivery of neuroactives, unique ligand–polymer conjugates, proposed advances in insect cell culture following from pharmaceutical cell biology, and laboratory or organ‐on‐a‐chip approaches. It is hoped that these concepts will stimulate novel thinking which may enable discovery of efficacious new neuroactive insecticides. © 2020 Society of Chemical Industry

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Characterization of cell lines derived from the southern armyworm, Spodoptera eridania

Cited by 4 publications

References 31 publications

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Pharmaceutical technologies with potential application to insecticide discovery^†

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Characterization of cell lines derived from the southern armyworm, Spodoptera eridania

Cited by 4 publications

References 31 publications

Prostaglandin A2 induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Prostaglandin A2 induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Pharmaceutical technologies with potential application to insecticide discovery†

Contact Info

Product

Resources

About

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Prostaglandin A₂ induces apoptosis in three cell lines derived from the fall armyworm, Spodoptera frugiperda

Pharmaceutical technologies with potential application to insecticide discovery^†