Characterization of Cellular Responses Involved in Reparative Dentinogenesis in Rat Molars

D’Souza, Rena N.; Bachman, T.A.; Baumgardner, K.R.; Butler, William T.; Litz, M.

doi:10.1177/00220345950740021301

Cited by 93 publications

(70 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding the regenerative process after tooth injuries such as cavity preparation and pulpal capping following pulp exposure, newly differentiated or surviving odontoblast-like cells express Dsp protein in addition to the immunoreaction in the tertiary dentin (14,25), suggesting that this protein is a reliable marker for regenerated odontoblasts and tertiary dentin. However, immunohistochemistry for Dsp is unable to determine the up-regulation of Dsp production in the afflicted odontoblasts, because Dsp protein has already been accumulated in their cytoplasm.…”

Section: Molar At Week 3 (Table 2)mentioning

confidence: 99%

“…The expression of Dsp mRNA in the odontoblasts became weak in intensity at Week 5 and almost disappeared at Week 8 in this study, suggesting the existence of odontoblasts that have vacated an active synthetic phase and committed to a quiescent phase. The term "resting odontoblasts" seems suitable for the definition of these cells (13,14).…”

Section: Classification Of Odontoblast-lineage Cells (Table 3)mentioning

confidence: 99%

See 1 more Smart Citation

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

View full text Add to dashboard Cite

Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

show abstract

Section: Molar At Week 3 (Table 2)mentioning

confidence: 99%

Section: Classification Of Odontoblast-lineage Cells (Table 3)mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

View full text Add to dashboard Cite

show abstract

“…23,24 ALP and OCN are initial-stage and late-stage markers of osteogenic differentiation, respectively. 25 These molecules are considered crucial markers for dentinogenesis Figure 4 analyses of mrNa expression of human dental pulp cells by quantitative real-time Pcr.…”

mentioning

confidence: 99%

Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

Lin¹,

Tu²,

Hsieh³

et al. 2017

IJN

View full text Add to dashboard Cite

Statin at appropriate concentrations has been shown to induce odontoblastic differentiation, dentinogenesis, and angiogenesis. However, using a carrier to control statin release might reduce toxicity and enhance its therapeutic effects. The aim of this study was to prepare poly( d , l -lactide- co -glycolide acid) (PLGA) nanoparticles that contain lovastatin for application in direct pulp capping. The PLGA–lovastatin particle size was determined using dynamic light scattering measurements and transmission electron microscopy. In addition, the release of lovastatin was quantified using a UV–Vis spectrophotometer. The cytotoxicity and alkaline phosphatase (ALP) activity of PLGA–lovastatin nanoparticles on human dental pulp cells were investigated. Moreover, a real-time polymerase chain reaction (PCR) assay, Western blot analysis, and an enzyme-linked immunosorbent assay (ELISA) were used to examine the osteogenesis gene and protein expression of dentin sialophosphoprotein (DSPP), dentin matrix acidic phosphoprotein 1 (DMP1), and osteocalcin (OCN). Finally, PLGA–lovastatin nanoparticles and mineral trioxide aggregate (MTA) were compared as direct pulp capping materials in Wistar rat teeth. The results showed that the median diameter of PLGA–lovastatin nanoparticles was 174.8 nm and the cumulative lovastatin release was 92% at the 44th day. PLGA–lovastatin nanoparticles demonstrated considerably a lower cytotoxicity than free lovastatin at 5, 9, and 13 days of culture. For ALP activity, the ALP amount of PLGA–lovastatin (100 μg/mL) was significantly higher than that of the other groups for 9 and 13 days of culture. The real-time PCR assay, Western blot analysis, and ELISA assay showed that PLGA–lovastatin (100 μg/mL) induced the highest mRNA and protein expression of DSPP, DMP1, and OCN in pulp cells. Histological evaluation of the animal studies revealed that MTA was superior to the PLGA–lovastatin in stimulating the formation of tubular dentin in an observation period of 2 weeks. However, in an observation period of 4 weeks, it was evident that the PLGA–lovastatin and MTA were competitive in the formation of tubular reparative dentin and a complete dentinal bridge.

show abstract

“…A striking feature of the comparison between primary odontoblast and odontoblast-like cell differentiation is the absence of involvement of dental epithelium in the latter situation 26) . Despite of this critical importance, parallel molecular mechanisms may exist between dental repair and morphogenesis in the embryo, as suggested that some molecular mechanisms are common to dental development and repair 27) .…”

Section: Discussionmentioning

confidence: 99%

The Role of Autonomous Wntless in Odontoblastic Differentiation of Mouse Dental Pulp Cells

Choi¹,

Kim²,

Ko³

et al. 2016

Journal of Korean Dental Science

View full text Add to dashboard Cite

ORIGINAL ARTICLEPurpose: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin. Materials and Methods:To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction.Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout.Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.

show abstract

Characterization of Cellular Responses Involved in Reparative Dentinogenesis in Rat Molars

Cited by 93 publications

References 28 publications

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

The Role of Autonomous Wntless in Odontoblastic Differentiation of Mouse Dental Pulp Cells

Contact Info

Product

Resources

About