Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification

Abstract

The aim of this work was to evaluate the biochemical features of the white-rot fungi Pycnoporus sanguineus cellulolytic complex and its utilization to sugarcane bagasse hydrolysis. When cultivated under submerged fermentation using corn cobs as carbon source, P. sanguineus produced high FPase, endoglucanase, β-glucosidase, xylanase, mannanase, α-galactosidase, α-arabinofuranosidase, and polygalacturonase activities. Cellulase activities were characterized in relation to pH and temperature. β-Glucosidase and FPase activities were higher at 55 °C, pH 4.5, and endoglucanase activity was higher at 60 °C, in a pH range of 3.5-4.0. All cellulase activities were highly stable at 40 and 50 °C through 48 h of pre-incubation. Crude enzymatic extract from P. sanguineus was applied in a saccharification experiment using acid-treated and alkali-treated sugarcane bagasse as substrate, and the hydrolysis yields were compared to that obtained by a commercial cellulase preparation. Reducing sugar yields of 60.4% and 64.0% were reached when alkali-treated bagasse was hydrolyzed by P. sanguineus extract and commercial cellulase, respectively. Considering the glucose production, it was observed that P. sanguineus extract and commercial cellulase ensured yields of 22.6% and 36.5%, respectively. The saccharification of acid-treated bagasse was lower than that of alkali-treated bagasse regardless of the cellulolytic extract. The present work showed that P. sanguineus has a great potential as an enzyme producer for biomass saccharification.

Selection and Characterization of a Native Pycnoporus sanguineus Strain as a Lignocellulolytic Extract Producer from Submerged Cultures of Various Agroindustrial Wastes

“…For Pycnoporus genus, it has been reported that cellulase production reached 20 U/mL from a P. coccineus IBB310 strain via the submerged fermentation of tangerine peels (Elisashvili et al 2011). For P. sanguineus PF-2, 0.06 U/mL production via the submerged fermentation of milled corn cobs was reported (Falkoski et al 2012). These results are very close to those of the present work.…”

“…In this case, banana peels were better natural inductors than leaf litter, wheat straw, or apple peels (Elisashvili et al 2008). For P. sanguineus PF-2 under submerged fermentation in media supplemented with milled corncobs, the level detected was 10 U/mL (Falkoski et al 2012), much higher than that in the present study for P. sanguineus CS2. The difference in these values may be due to media composition, culture conditions, or the origin of the strains.…”

Exploring the Natural Fungal Biodiversity of Tropical and Temperate Forests toward Improvement of Biomass Conversion

“…White-rot fungi mineralize cell wall components (cellulose, hemicelluloses, and lignins) and extensively degrade lignins (38). For instance, Pycnoporus is known to produce high FP endoglucanase, ␤-glucosidase, xylanase, mannanase, ␣-galactosidase, ␣-arabinofuranosidase, and polygalacturonase activities (11) as well as large amounts of laccases (22) and cellobiose dehydrogenase (CDH) (4), which have been shown to affect biomass conversion (4,11,25). It is now clear that fungi convert lignocellulose through a multienzymatic process involving numerous CAZymes and oxidative enzymes.…”

Enzymatic, Antioxidant, Antimicrobial, and Insecticidal Activities of Pleurotus pulmonarius and Pycnoporus cinnabarinus Grown Separately in an Airlift Reactor

“…Zymograms of cellulolytic isoenzymes from both species showed molecular masses ranging from 25 to 90 kDa (Quiroz-Castañeda et al 2009). Falkoski et al (2012) grew Pycnoporus sanguineus in SmF using corn cobs as a carbon source, and the resulting polygalacturonase, xylanase, FPase, endoglucanase, β-glucosidase, mannanase, α-galactosidase, and α-arabinofuranosidase activities were evaluated. Endoglucanase activity was higher at 60 °C, in a pH range of 3.5 to 4.0, and β-glucosidase and FPase activities were higher at 55 °C, pH 4.5.…”