Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.

Wang, Y; Zhang, W; Cao, Jun; McElroy, David; Wü, Ray

doi:10.1128/mcb.12.8.3399

Cited by 31 publications

(13 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It may further indicate that cells in root apical meristem may contain a specific protein factor that is essential in the recognition of the short promoter fragment. Such root-specific transcription factors have been previously described in both tobacco [23] and rice [39]. Thus, the specific expression pattern of the Osgrpl revealed in this study may be regulated by both positive and negative mechanisms.…”

Section: Mechanisms For Temporal and Spatial Regulation Of Osgrpl Genmentioning

confidence: 71%

Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation

Lei

Wü

1995

Plant Mol Biol

View full text Add to dashboard Cite

To study the expression and regulation of a rice glycine-rich cell wall protein gene, Osgrp1, transgenic rice plants were regenerated that contain the Osgrp1 promoter or its 5' deletions fused with the bacterial beta-glucuronidase (GUS) reporter gene. We report here a detailed histochemical analysis of the Osgrp1-Gus expression patterns in transgenic rice plants. In roots of transgenic rice plants, GUS expression was specifically located in cell elongation and differentiation regions, and no GUS expression was detectable in the apical meristem and the mature region. In shoots, GUS activity was expressed only in young leaves or in the growing basal parts of developing leaves, and little GUS activity was expressed in mature leaves or mature parts of developing leaves. In shoot apices, GUS activity was detected only in those leaf cells which were starting to expand and differentiate, and GUS expression was not detected in the apical meristem and the young meristematic leaf primordia. GUS activity was highly expressed in the young stem tissue, particularly in the developing vascular bundles and epidermis. Thus, the expression of the Osgrp1 gene is closely associated with cell elongation/expansion during the post-mitotic cell differentiation process. The Osgrp1-Gus gene was also expressed in response to wounding and down-regulated by water-stress conditions in the elongation region of roots. Promoter deletion analysis indicates that both positive and negative mechanisms are involved in regulating the specific expression patterns. We propose a simple model for the developmental regulation of the Osgrp1 gene expression.

show abstract

Section: Mechanisms For Temporal and Spatial Regulation Of Osgrpl Genmentioning

confidence: 71%

Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation

Lei

Wü

1995

Plant Mol Biol

View full text Add to dashboard Cite

show abstract

“…Here, we used the rice actin1 promoter (Wang et al, 1992) to constitutively overexpress the AtCKX2 gene and transformed moss protoplasts by PEGmediated direct gene transfer. The regeneration of CKX-overexpressing plants proved to be extremely difficult, and we were only able to isolate two stable transformants, tCKX7 and tCKX16.…”

Section: Discussionmentioning

confidence: 99%

Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins

Schwartzenberg

Núñez²,

Blaschke³

et al. 2007

Plant Physiology

107

View full text Add to dashboard Cite

Ultra-performance liquid chromatography-tandem mass spectrometry was used to establish the cytokinin profile of the bryophyte Physcomitrella patens (Hedw.) B.S.G.; of 40 analyzed cytokinins, 20 were detected. cis-Zeatin-riboside-O-glucoside, N 6 -(D 2 -isopentenyl)adenosine-5#-monophosphate (iPRMP), and trans-zeatin-riboside-O-glucoside were the most abundant intracellular cytokinins. In addition, the aromatic cytokinins N 6 -benzyladenosine (BAR), N 6 -benzyladenine, meta-, and orthotopolin were detected. Unexpectedly, the most abundant extracellular cytokinin was the nucleotide iPRMP, and its identity was confirmed by quadrupole time-of-flight mass spectrometry. The effects of overexpressing a heterologous cytokinin oxidase/ dehydrogenase (CKX; EC 1.4.3.18/1.5.99.12) gene (AtCKX2 from Arabidopsis [Arabidopsis thaliana]) on the intracellular and extracellular distribution of cytokinins was assessed. In cultures of CKX-transformed plants, ultra-performance liquid chromatography-tandem mass spectrometry measurements showed that there were pronounced reductions in the extracellular concentrations of-isopentenyl)adenosine (iPR), but their intracellular cytokinin concentrations were only slightly affected. In vitro and in vivo measured CKX activity was shown to be strongly increased in the transformants. Major phenotypic changes observed in the CKX-overexpressing plants included reduced and retarded budding, absence of sexual reproduction, and abnormal protonema cells. In bud-induction bioassays with wild-type Physcomitrella, the nucleotides iPRMP, trans-zeatin-riboside-5#-monophosphate, BAR monophosphate, and the cis-zeatin forms cZ and cZR had no detectable effects, while the activities displayed by other selected cytokinins were in the following order: iP . tZ . N 6 -benzyladenine . BAR . iPR . tZR . meta-topolin . dihydrozeatin . ortho-topolin. The results on wild type and CKX transgenics suggest that extracellular iP and iPR are the main cytokinins responsible for inducing buds in the bryophyte Physcomitrella. Cytokinin profile is discussed regarding the evolution of cytokinin biosynthetic pathways.Cytokinins play important roles as growth-regulating compounds in plants (Kieber, 2002). External applications of cytokinins to mosses have been shown to induce bud formation and, thus, the transition from filamentous, protonemic growth to the formation of gametophores (Bopp and Brandes, 1964;Reski and Abel, 1985). However, knowledge of the endogenous cytokinin profiles of mosses is incomplete, and it is unclear how their intracellular and extracellular distributions regulate developmental processes. We have therefore attempted to establish the cytokinin profile of Physcomitrella patens, a model organism for plant development and metabolism studies (Cove et al., 2006). Naturally occurring cytokinins are N 6 -substituted adenine derivatives bearing either an isoprenoid or an aromatic side chain. Isoprenoid forms include N 6 -(D 2 -isopentenyl)adenine (iP)-and zeatin (Z)-type cytokinins, which are characterized by the ...

show abstract

“…Insertion of SRS in the Ϫ459 position of the Act1 promoter alters the mode of regulation of this promoter by glucose. Previously, the Act1 promoter deletion to nucleotide Ϫ459 has been shown to still display high activity in rice protoplasts (53), suggesting that conversion of the promoter activity in response to glucose is not simply due to disruption of the promoter sequence by SRS. It is interesting to note that the absolute level of glucose starvation-induced expression of the Act1 promoter containing SRS was dramatically higher than that of the 35S minimal promoter fused to SRS (compare Fig.…”

Section: The Gc Box G Box and Tatcca Element Act Synergistically Inmentioning

confidence: 99%

Sugar Response Sequence in the Promoter of a Rice α-Amylase Gene Serves as a Transcriptional Enhancer

Lu¹,

Lim²,

Yu³

1998

Journal of Biological Chemistry

162

163

View full text Add to dashboard Cite

Expression of ␣-amylase genes in both rice suspension cells and germinating embryos is repressed by sugars and the mechanism involves transcriptional regulation. The promoter of a rice ␣-amylase gene ␣Amy3 was analyzed by both loss-and gain-of-function studies and the major sugar response sequence (SRS) was located between 186 and 82 base pairs upstream of the transcription start site. The SRS conferred sugar responsiveness to a minimal promoter in an orientation-independent manner. It also converted a sugar-insensitive rice actin gene promoter into a sugar-sensitive promoter in a dosedependent manner. Linker-scan mutation studies identified three essential motifs: the GC box, the G box, and the TATCCA element, within the SRS. Sequences containing either the GC box plus G box or the TATCCA element each mediated sugar response, however, they acted synergistically to give a high level glucose starvation-induced expression. Nuclear proteins from rice suspension cells binding to the TATCCA element in a sequence-specific and sugar-dependent manner were identified. The TATCCA element is also an important component of the gibberellin response complex of the ␣-amylase genes in germinating cereal grains, suggesting that the regulation of ␣-amylase gene expression by sugar and hormone signals may share common regulatory machinery.Sugar repression of gene expression is a fundamental and ubiquitous regulatory system for adjusting to changes in nutrient availability in both prokaryotic and eukaryotic cells. In microorganisms, glucose or other rapidly metabolizable carbon sources repress the expression of genes that code for enzymes related to the metabolism of other carbon sources. Our understanding of the mechanisms of sugar repression has been based largely on studies of microorganisms. In the case of Escherichia coli, a model to explain at the molecular level, the mechanism of sugar repression has been determined (1, 2). The mechanism of glucose repression in yeast is more complicated and is less understood than it is in E. coli (3-5). Studies using Saccharomyces cerevisiae mutants have revealed many of the components involved in the response to carbon catabolite repression (5), but it is still unclear how all of these components interact to regulate transcription. A universal signaling pathway which leads to the regulation of all glucose-repressible genes has yet to be determined.As in microorganisms, sugar repression of gene expression also allows plant cells to cope effectively with changes in the carbon sources present in their environment. However, in multicellular plants, feedback repression by excess sugars provides an additional mechanism for maintaining an economical balance between supply (source) and demand (sink) for carbohydrate allocation and utilization among tissues and organs (6 -8). Despite the fact that sugar repression of gene expression is likely a central control mechanism mediating energy homeostasis and carbohydrate distribution in plants, the molecular mechanism of sugar feedback regulation remain...

show abstract

Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene.

Cited by 31 publications

References 59 publications

Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation

Expression of the rice Osgrp1 promoter-Gus reporter gene is specifically associated with cell elongation/expansion and differentiation

Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins

Sugar Response Sequence in the Promoter of a Rice α-Amylase Gene Serves as a Transcriptional Enhancer

Contact Info

Product

Resources

About