Characterization of citrus germplasm including unknown variants by inter-simple sequence repeat (ISSR) markers

Shahsavar, Ali Reza; Izadpanah, K.; Tafazoli, E.; Tabatabaei, Badraldin Ebrahim Sayed

doi:10.1016/j.scienta.2006.12.039

Cited by 57 publications

(47 citation statements)

References 24 publications

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“…This higher dissimilarity between the lemon and acid lime trees was also noted by Shahsavar et al (2007) using ISSR markers to study the phylogenetic relationships between citrus species, clustering PAL with 'Galego' (80% similarity) and detecting lower similarity (approximately 64%) between the lemon tree and acid lime tree groups. As observed, the use of one type of marker does not exclude using another type of marker since markers can have different annealing regions; therefore, different markers can be used in various situations to detect different levels of polymorphisms, depending on the species.…”

Section: Resultssupporting

confidence: 59%

Variability analysis of 'Persian' acid lime tree selections using agronomic and molecular markers

Santos¹,

Passos²,

Filho³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. 'Persian' acid lime (PAL) is the most important triploid commercial citrus crop planted in the world. Little is known about the genetic variability of the selections used in Brazil. Therefore, 25 genotypes originating from the PAL, and three control species, Citrus sunki, C. limon, and C. aurantiifolia, were assessed using intersimple sequence repeat (ISSR) and inter-retrotransposon amplified polymorphism (IRAP) molecular markers and agronomic traits of the fruit. The dendrograms were designed using the mean Euclidean distance for the physicochemical attributes of the fruit (weight, length, diameter, peel color, peel thickness, number of seeds, juice yield, titratable acidity, soluble solids, and ratio) and the Jaccard distances using the data from the ISSR and IRAP molecular markers. In the physicochemical analysis, the genotypes were grouped according to species. The trait that contributed most to the diversity among accessions was the number of seeds. The 17 ISSR primers produced 69 polymorphic bands in the molecular analysis, and the seven IRAP Variability of 'Persian' acid lime selections primers generated 30 polymorphic bands. The markers detected polymorphisms within and among the PALs; two groups were formed within the PALs.

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Section: Resultssupporting

confidence: 59%

Variability analysis of 'Persian' acid lime tree selections using agronomic and molecular markers

Santos¹,

Passos²,

Filho³

et al. 2013

Genet. Mol. Res.

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show abstract

“…), as maternal parent, and sweet lime (C. limettioides Tan.). Shahsavar et al (2007) also found a close affinity between 'Bakraii' and sweet lime using ISSR markers. Our results also support a close relationship between 'Bakraii' and sweet lime.…”

Section: Ssr Unique Allelesmentioning

confidence: 70%

“…This cultivar is usually used as a rootstock for citrus cultivars especially Mexican lime in south of Iran. Its fruits are consumed occasionally despite their bitterness (Shahsavar et al 2007). Recently through SSR and PCR-RFLP markers, Golein et al (2012) found that 'Bakraii' is a hybrid between rough lemon (C. jambhiri Lush.…”

Section: Ssr Unique Allelesmentioning

confidence: 99%

“…Among many molecular markers, Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) markers have been used in citrus in a wide range of applications including cultivar identification (Fang and Roose 1997;Novelli et al 2000;Shahsavar et al 2007;Biswas et al 2010;Uzun et al 2010), phylogenetics (Pang et al 2003;Marak and Laskar 2010), zygotic and nucellar seedlings differentiation (Ruiz et al 2000;Oliveira et al 2002;Krueger and Roose 2003) and the construction of linkage maps for marker assisted breeding and map-based cloning of genes (Kijas et al 1997;Sanker and Moore 2001).…”

Section: Introductionmentioning

confidence: 99%

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Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Sharafi

Abkenar

Sharafi

et al. 2016

Physiol Mol Biol Plants

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Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime Bwitches' broom disease^.

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“…From each accession, four young leaves were taken, and total genomic DNA was extracted according to Doyle and Doyle with some modifications [4][5][6][7][8][9][10][11][12][13][14][15]. The leaves were ground to a fine powder in liquid nitrogen, and 200 mg of fresh ground leaves were mixed with 1 ml of preheated (65 °C) extraction buffer (2% hexadecyltrimethylammonium bromide [CTAB: Sigma H-5882], 1.4 M NaCl, 0.2% 2-mercaptoethanol, 20 mmol EDTA, 100 mmol Tris-HCl, pH 8.0).…”

Section: Dna Isolationmentioning

confidence: 99%

Botanical and Genetic Characterization of Citrus Maxima (Burm.) Merrill. F. Rutaceae

Sedeek

Kirollos

Michel

et al. 2016

Int J Pharm Pharm Sci

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Objective: Total investigation of the macro-and micromorphological features of Citrus maxima (Burm.) Merrill. In addition, DNA fingerprinting in this study was to estimate genetic polymorphism and relationships with another citrus viz: Citrus paradise and Citrus sinensis based on RAPD and ISSR markers. Methods:For the establishment of macro-and micromorphological investigation and genetic estimation, this study presents a total investigation of the macro-and micromorphological features of the roots, stems, leaves, flowers, fruits and seeds of the plants through microscopical investigation of the prepared entire, transverse sections and powdered forms of different organs under study. Furthermore, the DNA of the three plants was extracted from leaf samples and two types of DNA markers RAPD and ISSR were assayed.Results: Macro-and micromorphological features of different organs were investigated. On the other hand Genetic polymorphism and relationships among Citrus maxima, Citrus paradisi and Citrus sinensis was revealed. The highest genetic similarity was between Citrus maxima and Citrus paradisi while the lowest similarity was between Citrus paradisi and Citrus sinensis as revealed by RAPD, ISSR and combined data. Conclusion:For the present study, macro and micro-morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters for authentication of Citrus maxima (Burm.) Merrill.

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Characterization of citrus germplasm including unknown variants by inter-simple sequence repeat (ISSR) markers

Cited by 57 publications

References 24 publications

Variability analysis of 'Persian' acid lime tree selections using agronomic and molecular markers

Variability analysis of 'Persian' acid lime tree selections using agronomic and molecular markers

Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

Botanical and Genetic Characterization of Citrus Maxima (Burm.) Merrill. F. Rutaceae

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