Characterization of Clostridium difficile strains isolated from immunosuppressed inpatients in a hospital in Rio de Janeiro, Brazil

Balassiano, Ilana Teruszkin; Miranda, Karla Rodrigues; Boente, Renata F.; Pauer, Heidi; Oliveira, Ivi Cristina Menezes de; Santos-Filho, Joaquim dos; Amorim, Efigênia L.T.; Caniné, Gerson A.; Souza, Cristina F.; Gomes, Marisa Zenaide Ribeiro; Ferreira, Eliane de Oliveira; Brazier, Jon S.; Domingues, Regina Maria Cavalcanti Pilotto

doi:10.1016/j.anaerobe.2008.12.007

Cited by 30 publications

(22 citation statements)

References 18 publications

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“…In addition, vancomycin is considered clinically more effective and generates fewer relapse cases following CDI treatment in humans (SHAH et al, 2010); therefore, it is considered the best option by many clinicians. In addition to this study, only one other study in Brazil has evaluated antimicrobial susceptibility in six C. diffi cile strains isolated from humans with CDI; similar to this study, the isolates were also susceptible to metronidazole and vancomycin (BALASSIANO et al, 2009).…”

Section: Discussionmentioning

confidence: 89%

Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil

et al. 2014

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Section: Discussionmentioning

confidence: 89%

Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil

et al. 2014

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“…In Brazil, few data concerning the importance of CDI and the incidence of clonal types of C. difficile exist (Pinto et al 2003;Alcides et al 2007;Balassiano et al 2009). Therefore, the aim of this work was to isolate and characterize phenotypically and genotypically C. difficile strains from samples obtained from a University Hospital in Rio de Janeiro city, Brazil.…”

Section: Introductionmentioning

confidence: 99%

Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil

Balassiano

Santos-Filho

Vital-Brazil

et al. 2010

Antonie van Leeuwenhoek

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Clostridium difficile is an important nosocomial enteric pathogen and is the etiological agent of pseudomembranous colites. Recently, the rates of C. difficile infection (CDI) have increased worldwide, but in Brazil few data about this situation and the incidence of clonal types of C. difficile exist. This study aimed to isolate and characterize C. difficile strains from samples obtained of a university hospital (HUCFF) in Rio de Janeiro city, Brazil. CDI was identified by ELISA in 27.1% of HUCFF-in-patients enrolled in the study, and the bacterium was recovered from eight of these fecal samples. All strains, except one, presented tcdA and tcdB genes and presented neither the cdtA and cdtB genes nor any significant deletions in the tcdC gene. All strains were sensitive to metronidazole, vancomycin and moxifloxacin, and resistant to clindamycin, ciprofloxacin and levofloxacin. PCR-ribotyping and PFGE revealed four different clonal types among the isolates. The Brazilian PCR-ribotype 133 accounted for 50% of strains isolated, and PCR-ribotype 233 strains were obtained from 25% of the in-patients. The prevalence and resurgence of the Brazilian PCR-ribotype 133 among the hospitalized patients of HUCFF was established, and cross-infection of different patients associated to the same PCR-ribotypes was detected. Our results emphasize the importance of the diagnosis and control of CDI in order to prevent the emergence of specific clones that can lead to C. difficile-associated outbreaks in Brazilian hospitals.

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“…Another study (Balassiano et al, 2009) also attempted to characterize C. difficile strains isolated from hospitalized patients but focused on the adult population. All of the 21 patients enrolled in the study had undergone broadspectrum antibiotic therapy; three of the patients were HIV-positive and one had a central nervous system neoplasm.…”

Section: Brazilmentioning

confidence: 99%

Clostridium difficile: a problem of concern in developed countries and still a mystery in Latin America

Balassiano

Yates

Domingues

et al. 2012

Journal of Medical Microbiology

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Characterization of Clostridium difficile strains isolated from immunosuppressed inpatients in a hospital in Rio de Janeiro, Brazil

Cited by 30 publications

References 18 publications

Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil

Antimicrobial susceptibility of Clostridium difficile isolated from animals and humans in Brazil

Detection of cross-infection associated to a Brazilian PCR-ribotype of Clostridium difficile in a university hospital in Rio de Janeiro, Brazil

Clostridium difficile: a problem of concern in developed countries and still a mystery in Latin America

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