Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected from farms and from supermarkets in the Czech Republic

Vojkovská, Hana; Karpíšková, Renata; Orieskova, Maria; Drahovská, Hana

doi:10.1016/j.ijfoodmicro.2015.10.017

Cited by 55 publications

(42 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cronobacter sakazakii in numerous studies (Lee et al, 2012;Turcovsky et al, 2011;Vojkovska et al, 2016;Hochel et al, 2012). Our findings also revealed the predominance of this species.…”

Section: Most Commonly Isolated Species Was Determined To Besupporting

confidence: 79%

“…Accordingly Cronobacter spp. were detected in dust, soil and other environmental samples at various quantities (Turcovsky et al, 2011;Vojkovska et al, 2016;Jaradat et al, 2009;Lee et al, 2012;Killer et al, 2015;Wang et al, 2012). These findings revealed the crucial role of environmental contaminants upon the microbiologic safety of end products.…”

Section: Most Commonly Isolated Species Was Determined To Bementioning

confidence: 96%

“…are mostly associated with contaminated powdered infant formulas and some incidents were reported in various countries (Van Acker et al, 2001;Himelright et al, 2002;Weir, 2002;Caubilla-Barron et al, 2007). In addition, Cronobacter species were isolated from a variety of dried foods, instant food products and their ingredients and also from domestic environments and food production facilities and environmental samples Drudy et al, 2006;Turcovsky et al, 2011;Hochel et al, 2012;Wang et al, 2012;Lee et al, 2012;Li et al, 2014;Killer et al, 2015;Garbowska et al, 2015;Vojkovska et al, 2016). It has been stated that Cronobacter species were more commonly isolated from plant-based foods and food compounds rather than foods of animal origin (Baumgartner et al, 2009;Turcovsky et al, 2011;Hochel et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence and identification by multiplex polymerase chain reaction patterns of Cronobacter spp. isolated from plant-based foods

Aksu

Altunatmaz

Issa³

et al. 2016

Food Sci. Technol

View full text Add to dashboard Cite

Cronobacter spp. involves a group of opportunistic pathogens that cause meningitis in newborns, immunosuppressed individuals with a mortality rate of 50-80%. Seven species like C. sakazakii, C. malonaticus, C. muytjensii, C. turicensis, C. dublinensis, C. universalis, C. condimenti are included in this genus which has been a subject of research especially in the bacteriologic analysis of baby foods. However, since these species were detected also in prepared foodstuffs. The objective of this study was to assert the presence of Cronobacter spp. in foodstuff offered for sale in Turkey. A total of 151 prepared foodstuffs including a variety of spice, flour, instant soup were purchased from different sales points. The presence of Cronobacter spp. were investigated in these samples. Cronobacter suspected isolates which were obtained by microbiological analyses were confirmed by PCR targeted to gyrB gene and were then identified by multiplex PCR. Prevalence of Cronobacter spp was estimated to be 17.88%. Out of 27 Cronobacter spp. isolates obtained, 13(48.1%), 6(22.2%), 5(18.5%), 3(11.1%) belonged to C. sakazakii, C. muytjensii, C. turicensis, C. malonaticus species, respectively. Consequently, the presence of the bacteria in widely consumed foodstuff revealed that Cronobacter spp. is subject to monitoring due to its opportunistic nature in terms of public health concern.Keywords: Cronobacter spp.; flour; instant soup; PCR; spice.Practical Application: Cronobacter spp in plant-based foods may compose health risks.

show abstract

“…Cronobacter sakazakii in numerous studies (Lee et al, 2012;Turcovsky et al, 2011;Vojkovska et al, 2016;Hochel et al, 2012). Our findings also revealed the predominance of this species.…”

Section: Most Commonly Isolated Species Was Determined To Besupporting

confidence: 79%

Section: Most Commonly Isolated Species Was Determined To Bementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and identification by multiplex polymerase chain reaction patterns of Cronobacter spp. isolated from plant-based foods

Aksu

Altunatmaz

Issa³

et al. 2016

Food Sci. Technol

View full text Add to dashboard Cite

show abstract

“…; Vojkovska et al . ). Although the main source of C. sakazakii is considered to be infant formula, this is still unclear (Ye et al .…”

Section: Discussionmentioning

confidence: 97%

Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Tutar

Akıncı

Akyol

2018

Int J of Dairy Tech

View full text Add to dashboard Cite

Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes are important foodborne pathogens in milk and milk products, which are responsible for a variety of diseases that pose serious hazards to public health and food safety. The objective of this study was to develop a novel multiplex RTi‐PCR for the detection of B. melitensis, C. sakazakii and L. monocytogenes and to characterise the potential risk of these pathogens in raw milk and cheese. The raw milk (n = 25) and cheese samples (n = 20) were analysed by multiplex RTi‐PCR assay and detected for quantification of the three pathogens. In this study, B. melitensis, C. sakazakii and L. monocytogenes were simultaneously identified using BMEII0466, mms operon and hly as target genes, respectively. The multiplex RTi‐PCR assay that was developed showed good sensitivity and selectivity for the pathogenic microorganisms (r2 = 0.986–0.997). Multiplex RTi‐PCR results showed that most of the samples were contaminated with the pathogens screened.

show abstract

“…; Vojkovska et al . ). As a consequence, the detection of pathogenic Cronobacter in PIF has drawn a lot of attention all over the world.…”

Section: Introductionmentioning

confidence: 97%

Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas

et al. 2017

Int J of Dairy Tech

View full text Add to dashboard Cite

A combination method of real-time PCR and high-resolution melting (HRM) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct T m (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80°C. The detection limit ranged from 0.1 to 1 pg per reaction. Desiccated Cronobacter sakazakii and Cronobacter muytjensii of 2 cfu/25 g were successfully detected after 4-week storage at room temperature. The newly developed method provided a molecular tool for direct detection and simultaneous identification of pathogenic Cronobacter species in powdered infant formulae.

show abstract

Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected from farms and from supermarkets in the Czech Republic

Cited by 55 publications

References 54 publications

Prevalence and identification by multiplex polymerase chain reaction patterns of Cronobacter spp. isolated from plant-based foods

Prevalence and identification by multiplex polymerase chain reaction patterns of Cronobacter spp. isolated from plant-based foods

Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas

Contact Info

Product

Resources

About