Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4

Bhimba, B. Valentin; Franco, DA Agnel Defora; Jose, Geena Mary; Mathew, Jibi Merin; Joel, Elsa Lycias

doi:10.1016/s2221-1691(11)60031-2

Cited by 25 publications

(11 citation statements)

References 16 publications

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“…In the present study we used two different molecular markers for blood meal identification (cyt b DNA sequencing and PNOC sequencing). Cytochrome b gene has been commonly used for the blood meal identification due to their high copy numbers and sufficient genetic variation at the primary sequence level among vertebrate taxa [ 62 , 63 , 64 , 65 , 66 ]. Also more than 8 000 cytochrome b gene sequences of vertebrate animals are available in the GenBank/EMB/DDBJ Database [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

“…This may be explained by two hypotheses: the first one is the class of tested target locus (single or multicopy locus). Actually, PCR detection sensitivity was proved to be higher when targeting multicopy loci like cyt b mitochondrial DNA (8 000 copy per cell) [ 65 ] than when using a single copy gene. The second one is the specificity of the used primers.…”

Section: Discussionmentioning

confidence: 99%

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Blood Meal Analysis of Culicoides (Diptera: Ceratopogonidae) in Central Tunisia

et al. 2015

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To evaluate the host preferences of Culicoides species (Diptera: Ceratopogonidae) in Central Tunisia, we identified the source of blood meals of field collected specimens by sequencing of the cytochrome b (cyt b) mitochondrial locus and Prepronociceptine single copy nuclear gene. The study includes the most common and abundant livestock associated species of biting midges in Tunisia: C. imicola, C. jumineri, C. newsteadi, C. paolae, C. cataneii, C. circumscriptus, C. kingi, C. pseudojumineri, C. submaritimus, C. langeroni, C. jumineri var and some unidentified C. species. Analysis of cyt b PCR products from 182 field collected blood-engorged females’ midges revealed that 92% of them fed solely on mammalian species, 1.6% on birds, 2.4% on insects and 0.8% on reptiles. The blast results identified the blood origin of biting midges to the species level with exact or nearly exact matches (≥98%). The results confirm the presence of several Culicoides species, including proven vectors in Central Tunisia. Blood meal analyses show that these species will indeed feed on bigger mammals, thereby highlighting the risk that these viruses will be able to spread in Tunisia.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Blood Meal Analysis of Culicoides (Diptera: Ceratopogonidae) in Central Tunisia

et al. 2015

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“…In marine and estuarine environments, Aspergillus is one of several fungal genera often found in different hosts, from marine organisms to mangrove plants [ 3 28 ]. Aspergillus species ( A. flavus , A. niger , and A. nidulans ) have been isolated from leaves, twigs, and roots of several Rhizophoraceae mangroves such as R. mucronata , R. stylosa , and R. apiculata [ 14 46 ]. It has been reported that Aspergillus fungi from those mangrove species produce a wide variety of bioactive secondary metabolites that display activities against human microbial pathogens and cancer Hep2 and MCF7 cell lines [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…In Indonesia, mangrove fungal research has also focused on the exploration of mangrove fungal secondary metabolites for pharmaceutical applications [ 9 10 11 13 ], especially antibiotics; however, no research on their agrochemical applications has been reported to date. Many mangrove fungi isolated from mangrove species or genera have been used to extract biologically active compounds, several of which have exhibited insecticidal activities [ 14 15 16 ]. Recently, new active compounds, including 6-hydroxy-3-methylisochroman-5-carboxylic acid, mycoepoxydiene, 5-carboxylmellein, 5-methylmellein, pyrrole-2-carboxylic acid, and 3-methylhydantoin, were isolated from mangrove fungal culture media and shown to exhibit insecticidal activity against Helicoverpa armigera and Sinergasilus sp.…”

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confidence: 99%

Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

et al. 2015

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In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration (LC50) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

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“…If the three-dimensional structures could not be downloaded, they were created using MarvinSketch with the minimization parameters of the MMFF94 force field and a strict optimization limit. Various marine fungi bioactive compounds, whether known specifically as anticancer or not, were included in the marine database, resulting in 268 structures that were downloaded from PubChem and ChemSpider or created using MarvinSketch [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33].…”

Section: Preparation Of the Marine Databasementioning

confidence: 99%

Database Compilation and Virtual Screening of Secondary Metabolites Derived From Marine Fungi as Epidermal Growth Factor Receptor Tyrosine Abstract Kinase Inhibitors

Watty

Syahdi

Yanuar

2017

Asian J Pharm Clin Res

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Objective: Epidermal growth factor receptor (EGFR), a transmembrane protein with cytoplasmic kinase activity, transduces growth factor signaling from the extracellular space to the cell. EGFR downstream signaling increases proliferation and reduces apoptosis. Agents that are targeted at intracellular tyrosine kinase are tyrosine kinase inhibitor small molecules, which have a mechanism of action that affects adenosine triphosphate binding to the receptor. The exploration of bioactive compounds from marine materials, including marine fungi, has become a major interest lately for anticancer treatment. Methods:In this research, a database was created and in silico screening was conducted using AutoDock and Vina to obtain potential marine fungi bioactive compounds as EGFR-tyrosine kinase (EGFR-TK) inhibitors, which act as antiproliferative agents on tumor cell growth. Results:This research has concluded that the three marine fungi compounds with the lowest binding free energy, FU0015, FU0051, and FU0202, have great potential as inhibitors of EGFR-TK.Keywords: Marine fungi compounds, Epidermal growth factor receptor-tyrosine kinase, Virtual screening, AutoDock, Vina, Antiproliferative. INTRODUCTIONEpidermal growth factor receptor (EGFR) is an important target in the process of reducing tumor growth. More than 60% of non-small cell lung cancers express EGFR [1]. EGFR downstream signaling increases proliferation and angiogenesis and reduces apoptosis [2]. The overexpression of EGFR has been detected in the majority of human carcinomas [3].Tyrosine kinase inhibitors (TKIs) are one of four classes of anti-EGFR agents used for cancer therapy [4]. In 2015, gefitinib (one of the TKIs) was approved by the Food and Drug Administration (FDA) as an inhibitor of EGFR [5]. In addition to synthetic drugs, natural products have an important role in cancer therapy. Chemical compounds from natural products can be potential new medicines. The exploration of bioactive compounds from marine materials, including marine fungi, has become a major activity lately for anticancer treatment. Currently, there are anticancer compounds from marine fungal metabolites that have entered clinical trials. One of the alkaloid compounds, plinabulin, is a modified structure based on the (-)-phenylahistin metabolites of the terrestrial and marine fungi Aspergillus ustus [6]. As the development of microbiology technology increases in the future, the availability of microorganisms such as marine fungi will increase and the costs of production will be reduced. Therefore, bioactive compounds of marine fungi have the potential to be new drug compounds [7]. A database of active compounds was created to ease in silico and in vitro research further.In silico research can support conventional research (wet lab) that is relatively expensive and takes time [8]. AutoDock (Automatic Docking) is widely used to predict the complex of a biomolecular structure or to perform functional analysis and molecular design. AutoDock uses a grid-based method to rapidly evalu...

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Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4

Cited by 25 publications

References 16 publications

Blood Meal Analysis of Culicoides (Diptera: Ceratopogonidae) in Central Tunisia

Blood Meal Analysis of Culicoides (Diptera: Ceratopogonidae) in Central Tunisia

Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

Database Compilation and Virtual Screening of Secondary Metabolites Derived From Marine Fungi as Epidermal Growth Factor Receptor Tyrosine Abstract Kinase Inhibitors

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