Characterization of DMPC bilayers and multilamellar islands on hydrophobic self-assembled monolayers of ODS/Si(100) and mixed ODS-DDS/Si(100)

Brechling, Armin; Sundermann, Michael; Kleineberg, Ulf; Heinzmann, Ulrich

doi:10.1016/s0040-6090(03)00351-1

Cited by 20 publications

(21 citation statements)

References 22 publications

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“…A multistep immersion process has been used to create layers of dimyristoylphosphatidylcholine (DMPC) on the surface of silica plates with covalently attached octadecyl chains. ( 479 ) The uneven coverage of the hydrophobic surface and the formation of multilamellar islands make this technique unsuitable for the exact bilayer partitioning studies where a well-defined system is needed. The Langmuir−Blodgett technique( 480 ) produced a phospholipid monolayer on the surface of gold-coated plates with covalently attached octadecyl chains.…”

Section: Individual Steps Vs Propertiesmentioning

confidence: 99%

Modeling Kinetics of Subcellular Disposition of Chemicals

Baláž

2009

Chem. Rev.

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Section: Individual Steps Vs Propertiesmentioning

confidence: 99%

Modeling Kinetics of Subcellular Disposition of Chemicals

Baláž

2009

Chem. Rev.

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“…A multistep immersion technique has been used to create layers of dimyristoylphosphatidylcholine (DMPC) on the surface of silica plates with covalently attached octadecyl chains. 29 The uneven coverage of the hydrophobic surface and the formation of multilamellar islands make this technique unsuitable for exact bilayer partitioning studies where a well-defined system is needed. The Langmuir-Blodgett technique 30 produced a phospholipid monolayer on the surface of gold-coated plates with covalently attached octadecyl chains.…”

Section: Supported Monolayer Systemsmentioning

confidence: 99%

Drug-Membrane Interactions Studied in Phospholipid Monolayers Adsorbed on Nonporous Alkylated Microspheres

et al. 2007

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Characterization of interactions with phospholipids is an integral part of the in vitro profiling of drug candidates because of the roles the interactions play in tissue accumulation and passive diffusion. Currently used test systems may inadequately emulate the bilayer core solvation properties (immobilized artificial membranes [IAM]), suffer from potentially slow transport of some chemicals (liposomes in free or immobilized forms), and require a tedious separation (if used for free liposomes). Here the authors introduce a well-defined system overcoming these drawbacks: nonporous octadecylsilica particles coated with a self-assembled phospholipid monolayer. The coating mimics the structure of the headgroup region, as well as the thickness and properties of the hydrocarbon core, more closely than IAM. The monolayer has a similar transition temperature pattern as the corresponding bilayer. The particles can be separated by filtration or a mild centrifugation. The partitioning equilibria of 81 tested chemicals were dissected into the headgroup and core contributions, the latter using the alkane/water partition coefficients. The deconvolution allowed a successful prediction of the bilayer/water partition coefficients with the standard deviation of 0.26 log units. The plate-friendly assay is suitable for high-throughput profiling of drug candidates without sacrificing the quality of analysis or details of the drug-phospholipid interactions.

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“…This finding is not consistent with the deposition of a DMPC bilayer structure, where an adlayer of ca. 60 Å for a hydrated bilayer would be expected [39], along with a water contact angle of ca. 50° [10].…”

Section: Resultsmentioning

confidence: 97%