Characterization of dwarf and narrow leaf (<i>dnl-4</i>) mutant in rice

Bae, Ki-Deuk; Um, Taeyoung; Yang, Won Tae; Park, Tae-Hyeon; Hong, So-Yeon; Kim, Kyung–Min; Chung, Youngjin; Yun, Dae‐Jin; Kim, Doh-Hoon

doi:10.1080/15592324.2020.1849490

Cited by 18 publications

(12 citation statements)

References 39 publications

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“…To analyze the fructokinase family genes, we identified 15 candidate genes coding pfkB domain proteins in the rice genome using HMMER 3.1 (Table S2). Among them, eight genes (Os01g0894300, Os08g0113100, Os01g0851000, Os03g0602600, Os03g0737701, Os05g0185700, Os01g0105900, Os10g0573000) had been reported as OsFRK1 , OsFRK2 , OsFLN1 , OsFLN2 , OsMIK , OsPUKI , OsDNL-4, and OsPFKB1 , respectively. Interestingly, we found that Os06g0232200 was close to OsFRK2 and shared the same conserved motif with OsFRK1 and OsFRK2 (Figures a and S1).…”

Section: Resultsmentioning

confidence: 99%

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Zhang

Tan

Chen

et al. 2023

J. Agric. Food Chem.

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Plant fructokinase (FRK) guarantees the growth and development of higher plants by participating in carbohydrate metabolism. In this study, a new fructokinase, OsFRK3, was identified using bioinformatics analysis, enzyme assay, bacterial growth assay, and yeast complementation test. Then, we created OsFRK3 knockout transgenic lines (osfrk3-1 and osfrk3-2) by the CRISPR/Cas9 technology. We found that the 1000-grain weight decreased notably (approximately −3.6% and −6.1%, respectively) in osfrk3-1 and osfrk3-2. Evidently decreased grain width, grain thickness, and endosperm filling rate were detected in the osfrk3 mutants (osfrk3-1 and osfrk3-2) compared with those of the WT. In addition, the content of seed total starch was significantly decreased by 3.42 and 4.80% in osfrk3 lines, compared with that in the WT. The level of maltose was significantly reduced in the mutants, while that of sucrose and fructose was obviously increased in the mutants. The transcript levels of OsGBSS1, OsBEIIb, OsPFP1β, and OsAGPL1 were significantly decreased in the osfrk3 mutants. These results suggest that OsFRK3 may positively regulate the accumulation of starch through influencing the sugar metabolism.

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Section: Resultsmentioning

confidence: 99%

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Zhang

Tan

Chen

et al. 2023

J. Agric. Food Chem.

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“…Dwarf mutants are crucial to understanding the regulatory mechanisms for plant height, development, and productivity ( Bae et al., 2021 ) because they can be planted at high density and are resistant to lodging ( Zhu et al., 2019 ; Sun et al., 2020 ). In addition, a class of plant hormones such as GA, BR, and cytokinin were reported to regulate cell elongation and division that could cause the dwarf phenotype when the level of hormones was abnormal, and many dwarf mutants have been found in various crops such as rice sd1 , wheat Rht-B1b/D1b and cucumber Csdw ( Wei et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Fourteen dwarf mutants with short internodes and small leaf sizes were explored in this study. Previous studies reported that plant height-related genes are associated with leaf sizes such as OsNAL1 , OsNAL7 , and OsDNL-4 ( Bae et al., 2021 ). Similar to recent studies, six mutants of line 681, 942, 1372, 1833, 2194, and 3210 have short plant heights and small leaf sizes.…”

Section: Discussionmentioning

confidence: 99%

Construction of a density mutant collection in bitter gourd via new germplasms innovation and gene functional study

Wang

et al. 2022

Front. Plant Sci.

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Although a few studies have elucidated the creation of bitter gourd mutants, the suitable concentration and duration of ethyl methanesulfonate (EMS) mutagenesis have not been determined. In this study, mutant collection was conducted to create new germplasms and widen genetic diversity. By employing the seeds of the inbred line Y52 as the mutagenic material, EMS as the mutagen, and the suitable mutagenic conditions for bitter gourd seeds (EMS concentration 0.2%, mutagenic time 10 h), we mutated 10,000 seeds and acquired 3223 independent M1 lines. For the randomly selected 1000 M2 lines, 199 M2 lines with visible phenotypes were found, and 167 M2 lines were mutants of fruit shape, size, and tubercles. Furthermore, fourteen dwarf, eleven leaf color, five leaf shape, and eight meristem defect mutants were discovered in this mutant collection. In addition, three lines of 1253, 2284, and 3269 represented recessive mutants crossed with Y52. Furthermore, the yellow leaf lines of 2284 and 3269 were not mutated at the same gene locus. This study constructed a mutant collection through innovative new germplasms and provided valuable resources for bitter gourd breeding and functional gene research.

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“…Different stages of leaves, panicles and roots were freshly collected from the mutants and wild type plants (as a negative control) and then treated with staining solution (0.02M 5-bromo-4-chloro-3-indolyl-bb-D-glucuronide, 0.1M NaH 2 PO 4 , 0.25M ethylenediaminetetraacetic acid (EDTA), 5mM potassium ferricyanide, 5mM potassium ferrocyanide, 1.0% (v:v) Triton X-100, pH 7.0) and incubated overnight at 37°C. Cross section of leaf blades sample was fixed in FAA and then analysis was performed as previously described method ( Bae et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Gibberellic acid sensitive dwarf encodes an ARPC2 subunit that mediates gibberellic acid biosynthesis, effects to grain yield in rice

Hong

Han

et al. 2022

Front. Plant Sci.

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The plant hormone gibberellic acid (GA) is important for plant growth and productivity. Actin-related proteins (ARPs) also play central roles in plant growth, including cell elongation and development. However, the relationships between ARPs and GA signaling and biosynthesis are not fully understood. Here, we isolated OsGASD, encoding an ARP subunit from rice (Oryza sativa), using the Ac/Ds knockout system. The osgasd knockout (Ko) mutation reduced GA3 content in shoots as well as plant growth and height. However, GA application restored the plant height of the osgasd Ko mutant to a height similar to that of the wild type (WT). Rice plants overexpressing OsGASD (Ox) showed increased plant height and grain yield compared to the WT. Transcriptome analysis of flag leaves of OsGASD Ox and osgasd Ko plants revealed that OsGASD regulates cell development and the expression of elongation-related genes. These observations suggest that OsGASD is involved in maintaining GA homeostasis to regulate plant development, thereby affecting rice growth and productivity.

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Characterization of dwarf and narrow leaf (dnl-4) mutant in rice

Cited by 18 publications

References 39 publications

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Construction of a density mutant collection in bitter gourd via new germplasms innovation and gene functional study

Gibberellic acid sensitive dwarf encodes an ARPC2 subunit that mediates gibberellic acid biosynthesis, effects to grain yield in rice

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