Characterization of E. coli Phylogroups Causing Catheter-Associated Urinary Tract Infection

Abstract

Purpose Characterization of different uropathogenic E. coli (UPEC) phylogroups is crucial to understand pathogenesis of urinary tract infection (UTI). The objective of our study was to evaluate the antibiotic resistance pattern, biofilm formation and pathogenicity islands (PAIs) of UPEC phylogroups isolated from catheter-associated UTI (CAUTI) compared to community UTI (Com-UTI). Patients and Methods This study included 90 UPEC strains recovered from CAUTI and Com-UTI. Antimicrobial susceptibility was tested by the Kirby–Bauer method and extended spectrum beta-lactamase (ESBL) production was confirmed using the combined disk. The biofilm formation was tested using the microtiter plate assay. Main E. coli phylogroups (A, B1, B2 and D) were detected by multiplex PCR and 2 multiplex PCR detected the 8 PAIs. Results Antibiotic resistance of UPEC strains showed a similar high resistance in CAUTI and Com-UTI. Isolates from CAUTI significantly produced biofilm higher than Com-UTI strains (68.9% vs 44.4%). In CAUTI and Com-UTI isolates, phylogroup A was the commonest (53.3% vs 48.9%, respectively). PAI IV536 was the most common in the strains from CAUTI (71.1%) and Com-UTI (73.3%). No significant relationship was detected between the studied characters and different phylogroups except the significant resistance to cefotaxime, ceftazidime and aztreonam among phylogroups from CAUTI isolates. Conclusion Increased antibiotic resistance and ESBLs were detected in UPEC strains from CAUTI and Com-UTI. The strains from CAUTI significantly produced biofilm higher than Com-UTI strains. Phylogroup A was the predominate phylogroup and PAI IV536 was the most prevalent marker in all phylogroups from both types of UTI.

Phylotypic Profiling, Distribution of Pathogenicity Island Markers, and Antimicrobial Susceptibility of Escherichia coli Isolated from Retail Chicken Meat and Humans

“…These findings were in agreement with previous reports on humans in Iran [ 53 ], China [ 55 ], Sweden [ 36 ], and Spain [ 56 ]. A similar distribution of PAIs with a predominance of PAI IV 536 was reported in many studies [ 12 , 55 , 56 , 57 , 58 , 59 ].…”

Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital

“…In other Egyptian governorates, such as Giza and Dakahlia, a similar phylogenetic distribution of UPEC was reported [ 106 ]. However, studies performed on isolates from University Hospitals of Mansoura and Minya cities in Egypt revealed that the predominant group in UPEC was group A, followed by group B2 and then D [ 107 , 108 ]. On the other hand, a recent study found that phylogroup B2 prevailed in UPEC isolated in the Minya governorate, followed by group F [ 56 ].…”

The Antibiofilm Effects of Antimony Tin Oxide Nanoparticles against Polymicrobial Biofilms of Uropathogenic Escherichia coli and Staphylococcus aureus

“…S. aureus adheres to uroepithelial cells and forms biofilms within the urinary bladder [ 6 ]. Moreover, dual-species biofilms formed by UPEC and S. aureus have been reported to be responsible for catheter-associated UTIs [ 7 , 8 ]. However, despite the well-established link between bacteria and UTIs, few studies have explored the system of dual-species biofilms [ 9 , 10 ].…”