Characterization of Enterococcus faecium E86 bacteriocins and their inhibition properties against Listeria monocytogenes and vancomycin-resistant Enterococcus

Farias, Felipe Miceli de; Teixeira, Lúcia Martins; Vallim, Deyse Christina; Bastos, Maria do Carmo de Freire; Miguel, Marco Antônio Lemos; Bonelli, Raquel Regina

doi:10.1007/s42770-021-00494-3

Cited by 12 publications

(7 citation statements)

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“…The number of studies assessing the bacteriocinogenic activity against VRE is limited, but increasingly growing along with the recent boom of microbiome research studies [13]. Farias et al (2021) tested the potency of EntP against 14 VanA E. faecium and E. faecalis and concluded that all the VRE isolates were sensitive to the bacteriocin [105]. Similarly, Phumisantiphon et al (2017) tested the antimicrobial activity of bacteriocin EF478, produced by E. faecalis 478, against 68 MDRE and VRE clinical isolates, and found that EF478 was able to inhibit 41% of them [36].…”

Section: Use Of Bacteriocins To Fight Against Vre Human Infectionsmentioning

confidence: 99%

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

et al. 2021

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Enterococcus spp. are one of the most frequent producers of bacteriocins (enterocins), which provides them with an advantage to compete in their natural environment, which is the gut of humans and many animals. The enterocins’ activity against microorganisms from different phylogenetic groups has raised interest in Enterococcus spp. in different contexts throughout the last decades, especially in the food industry. Nevertheless, some species can also cause opportunistic life-threatening infections and are frequently multidrug-resistant (MDR). Vancomycin-resistant Enterococcus (VRE), in particular, are an ongoing global challenge given the lack of therapeutic options. In this scenario, bacteriocins can offer a potential solution to this persistent threat, either alone or in combination with other antimicrobials. There are a handful of studies that demonstrate the advantages and applications of bacteriocins, especially against VRE. The purpose of this review is to present a current standpoint about the dual role of Enterococcus spp., from important producers to targets needed to be controlled, and the crucial role that enterocins may have in the expansion of enterococcal populations. Classification and distribution of enterocins, the current knowledge about the bacteriocinome of clinical enterococci, and the challenges of bacteriocin use in the fight against VRE infections are particularly detailed.

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Section: Use Of Bacteriocins To Fight Against Vre Human Infectionsmentioning

confidence: 99%

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

et al. 2021

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“…Two of the previously mentioned four isolates contained another bacteriocin gene family (Subtilosin). These results are considered very interesting since the presence of multiple structural genes encoding for different types of bacteriocins has been reported in numerous lactic acid bacterial genera, with some isolates containing as many as four distinct bacteriocin genes (Henning et al, 2015;Farias et al, 2021). However, to our knowledge, this is the first time that Bacillus amyloliquefaciens isolates with multiple bacteriocin genes have been reported.…”

Section: Discussionmentioning

confidence: 59%

Diversity of Bacteriocin-encoding Gene Families and The Activity Spectrum among Bacillus amyloliquefaciens Isolates

et al. 2022

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B ACTERIOCINS are considered as ideal candidates for several health care applications due to their limited range of activity and rapid degradability by proteolytic enzymes. Eight bacteriocin-producing Bacillus amyloliquefaciens isolates were screened by polymerase chain reaction (PCR) using four sets of primers designed specifically to detect bacteriocin-producing genes on their chromosomes. Gene encoding for Amylocyclicin was detected in four isolates. A phylogenetic data analysis of the four Amylocyclicin-predicted proteins placed them in a separate node with their closest relatives, B. amyloliquefaciens and Bacillus velezensis strain FZmhtB, which until recently, was a member of the B. amyloliquefaciens species. Surprisingly, Subtilosin producing gene was detected in two of the previously mentioned isolates indicating that they contain multiple bacteriocin encoding genes, an unusual phenomenon for Bacillus amyloliquefaciens isolates. The remaining four isolates lacked any known bacteriocin gene family and are anticipated to contain novel gene types. The most potent of these four isolates was chosen for further large-scale production and extraction of its bacteriocin. Antibacterial activity of the extracted bacteriocin was detected in the protein fraction under the membrane cut-off value of <10,000kDa against gram-negative and gram-positive indicator bacterial isolates, with a larger average inhibition zone diameter observed for the gram-positive isolate. Furthermore, SDS-PAGE analysis of the partially purified active bacteriocin fraction revealed a protein fragment with a relative molecular weight between 7 and 7.5kDa. The PCR assay in this study provided coverage for all known B. amyloliquefaciens bacteriocins allowing the quick and easy screening for the presence of bacteriocin-encoding genes.

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“…Given bacteriocins’ low toxicity and bactericidal activity, as well as the rise of antibiotic-resistant bacteria, bacteriocins may be a novel potential solution for combating antibiotic-resistant infections [ 38 ]. A bacteriocinogenic gene cluster of E. faecium E86 encoding enterocin P (EntP) (bacteriocins produced by Enterococcus ) was able to inhibit the growth of Listeria monocytogenes and VRE strains [ 39 ].…”

Section: Probiotic Potential (Efficacy Of Enterococcal Strains)mentioning

confidence: 99%

“…They found that all tested L. monocytogenes and enterococcal strains were sensitive to EntP activity. In addition, EntP demonstrated bacteriolytic activity against pathogenic enterococcal strains, causing continuous growth reduction [ 39 ]. Bacteriocin producers may also stimulate the growth of beneficial bacterial species in the gastrointestinal tract.…”

Section: Probiotic Potential (Efficacy Of Enterococcal Strains)mentioning

confidence: 99%

Evaluation of Enterococcal Probiotic Usage and Review of Potential Health Benefits, Safety, and Risk of Antibiotic-Resistant Strain Emergence

Im,

Lee,

Kim

et al. 2023

Antibiotics

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Enterococci are often used in probiotics but can also cause nosocomial infections. As such, enterococcal consumption may have beneficial health effects, but a thorough evaluation of virulence absence and risk of antibiotic resistance spread is needed at the strain level. This article reviewed ten online health product shopping websites in the US. On these websites, 23 probiotic products using enterococci were found across 12 companies. In addition, this article reviewed studies that demonstrated the probiotic potential of enterococcal consumption (e.g., gastrointestinal and respiratory disease, hyperlipidemia alleviation, as well as infection prevention). To investigate the safety aspects of enterococci, the present work examined studies evaluating virulence factors and antibiotic resistance. Furthermore, this article assessed research that explored these virulent factors, specifically in probiotics containing enterococci, as well as the potential transfer mechanism of their antibiotic resistance. Based on reviewed data, enterococcal probiotic consumption has been proven beneficial for conditions or symptoms of multiple diseases without any apparent adverse effects. However, due to the plasmid- or transposon-mediated gene transfer ability of enterococci, surveillance monitoring and further studies regarding enterococcal consumption are warranted. Future studies that identify enterococcal strains safe to use in probiotics without virulence factors and antibiotic resistance are imperative for evidence-based decisions by health organizations and government agencies.

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Characterization of Enterococcus faecium E86 bacteriocins and their inhibition properties against Listeria monocytogenes and vancomycin-resistant Enterococcus

Cited by 12 publications

References 50 publications

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

Enterococcus spp. as a Producer and Target of Bacteriocins: A Double-Edged Sword in the Antimicrobial Resistance Crisis Context

Diversity of Bacteriocin-encoding Gene Families and The Activity Spectrum among Bacillus amyloliquefaciens Isolates

Evaluation of Enterococcal Probiotic Usage and Review of Potential Health Benefits, Safety, and Risk of Antibiotic-Resistant Strain Emergence

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