Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16

Wang, Shuning; Liu, Zhen; Tang, Hong Zhi; Meng, Jing; Xu, Ping

doi:10.1099/mic.0.2006/005223-0

Cited by 100 publications

(98 citation statements)

References 43 publications

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“…Earlier research suggested that succinic acid was produced during the formation of DHP in HSP metabolization by whole cells of P. putida S16, but succinic semialdehyde has never been reported (22,41). Succinic semialdehyde ([M-H] Ϫ peak at m/z 101) was produced during enzymatic degradation, which was verified with the commercial standard by LC-MS analysis.…”

Section: Discussionmentioning

confidence: 65%

“…The metabolic pathway of nicotine by strain P. putida S16 has been previously proposed based on the results of nuclear magnetic resonance, Fourier-transform infrared and UV spectroscopy, GC-MS, and high-resolution MS analyses (41). However, the pathway was not characterized at the genetic level.…”

Section: Discussionmentioning

confidence: 99%

“…The qualitative analysis of nicotine, P, SP, HSP, and DHP was performed by using analytical thin-layer chromatography (TLC) according to published procedures (40,41). Quantitative data of SP, HSP, and DHP were obtained by high-performance liquid chromatography (HPLC) analysis comparing the retention times and peak areas with those of standards.…”

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confidence: 99%

“…The nicotine degradation pathway of strain S16 was proposed based on the identification of intermediates produced by resting cells and crude extracts (41). Our efforts were directed toward confirmation of the pyrrolidine pathway in strain S16 by comprehensive characterization of the intermediates P, pseudooxynicotine, 3-succinoylpyridine (SP), 6-hydroxy-3-succinoylpyridine (HSP), and 2,5-dihydroxypyridine (DHP) (40,41). Knowledge of the genes involved in nicotine metabolism in Pseudomonas will have applications for detoxification of the tobacco wastes ␤-D-galactopyranoside; 20 g ml Ϫ1 ).…”

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confidence: 99%

“…MS analysis was performed on an API 4000 mass spectrometer under both negative-and positiveion turbo ion spray ionization mode. GC-MS analysis of P, SP, HSP, and DHP was performed as previously reported (41).…”

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confidence: 99%

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A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16

Tang

Wang

et al. 2008

Appl Environ Microbiol

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Previous research suggested that Pseudomonas spp. may attack the pyrrolidine ring of nicotine in a way similar to mammalian metabolism, resulting in the formation of pseudooxynicotine, the direct precursor of a potent tobacco-specific lung carcinogen. In addition, the subsequent intermediates, 6-hydroxy-3-succinoylpyridine (HSP) and 2,5-dihydroxypyridine (DHP) in the Pseudomonas nicotine degradation pathway are two important precursors for drug syntheses. However, there is little information on the molecular mechanism for nicotine degradation via the pyrrolidine pathway until now. In this study we cloned and sequenced a 4,879-bp gene cluster involved in nicotine degradation. Intermediates N-methylmyosmine, pseudooxynicotine, 3-succinoylpyridine, HSP, and DHP were identified from resting cell reactions of the transformant containing the gene cluster and shown to be identical to those of the pyrrolidine pathway reported in wild-type strain Pseudomonas putida S16. The gene for 6-hydroxy-3-succinoylpyridine hydroxylase (HSP hydroxylase) catalyzing HSP directly to DHP was cloned, sequenced, and expressed in Escherichia coli, and the purified HSP hydroxylase (38 kDa) is NADH dependent. DNA sequence analysis of this 936-bp fragment reveals that the deduced amino acid shows no similarity with any protein of known function.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16

Tang

Wang

et al. 2008

Appl Environ Microbiol

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Characterization and discrimination of microbial community and co‐occurrence patterns in fresh and strong flavor style flue‐cured tobacco leaves

Zhang

et al. 2019

MicrobiologyOpen

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Fermentation, also known as aging, is vital for enhancing the quality of flue-cured tobacco leaves (FTLs). Aged FTLs demonstrate high-quality sensory characteristics, while unaged FTLs do not. Microbes play important roles in the FTL fermentation process.However, the eukaryotic microbial community diversity is poorly understood, as are microbial associations within FTLs. We aimed to characterize and compare the microbiota associated with two important categories, fresh and strong flavor style FTLs, and to reveal correlations between the microbial taxa within them. Based on 16S and 18S rRNA Illumina MiSeq sequencing, the community richness and diversity of prokaryotes were almost as high as that of eukaryotes. The dominant microbes of FTLs belonged to seven genera, including Pseudomonas, Bacillus, Methylobacterium, Acinetobacter, Sphingomonas, Neophaeosphaeria, and Cladosporium, of the Proteobacteria, Firmicutes, and Ascomycota phyla. According to partial least square discriminant analysis (PLS-DA), Xanthomonas, Franconibacter, Massilia, Quadrisphaera, Staphylococcus, Cladosporium, Lodderomyces, Symmetrospora, Golovinomyces, and Dioszegia were significantly positively correlated with fresh flavor style FTLs, while Xenophilus, Fusarium, unclassified Ustilaginaceae, Tilletiopsis, Cryphonectria, Colletotrichum, and Cyanodermella were significantly positively correlated with strong flavor style FTLs. Network analysis identified seven hubs, Aureimonas, Kocuria, Massilia, Brachybacterium, Clostridium, Dietzia,and Vishniacozyma, that may play important roles in FTL ecosystem stability, which may be destroyed by Myrmecridium. FTL microbiota was found to be correlated with flavor style. Species present in lower numbers than the dominant microbes might be used as microbial markers to discriminate different flavor style samples and to stabilize FTL microbial communities. This research advances our understanding of FTL microbiota and describes a means of discriminating between fresh and strong flavor FTLs based on their respective stable microbiota. K E Y W O R D Sco-occurrence patterns, flue-cured tobacco leaf, Illumina MiSeq sequencing, microbial community, network analysis, partial least squares discriminant analysis

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Phylogenetic Affiliation of Pseudomonas sp. MO2, a Novel Polyhydroxyalkanoate-Synthesizing Bacterium

Sharma

Zhang

et al. 2015

Microbial Factories

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Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16

Cited by 100 publications

References 43 publications

A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16

A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16

Characterization and discrimination of microbial community and co‐occurrence patterns in fresh and strong flavor style flue‐cured tobacco leaves

Phylogenetic Affiliation of Pseudomonas sp. MO2, a Novel Polyhydroxyalkanoate-Synthesizing Bacterium

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