2014
DOI: 10.4236/jbm.2014.24009
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Characterization of Esterases of Tamarindus indica Seeds

Abstract: Germinating seeds of Tamarindus indica synthesizes various enzymes which are required for the degradation of seed reserves such as xyloglucans, fatty acid esters and proteins. Among these, esterases, belonging to a group of hydrolytic enzymes catalyze the hydrolysis of various types of esters. They play an important role in cell expansion as well as detoxification of xenobiotics and many agrochemicals and insecticides. The esterases are extracted from the germinating tamarind seeds using 50 mM phosphate buffer… Show more

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Cited by 5 publications
(6 citation statements)
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“…(Bagul et al, ) depicted that the phytic acid present in the tamarind seed might inhibit the digestive enzymes in human by forming a phytate‐protein and phytate‐mineral‐protein complexes. Also esterases (belongs to the group of hydrolytic enzymes) was responsible for the degradation of seed reserves such as xyloglucan (Kantharaju & Siddalinga Murthy, ). So the enzymes and low molecular weight compounds were inactivated by suspending in ethanol (purity 99.8%) in a ratio of 1:3 (seeds: ethanol) at 70°C for 15 min.…”
Section: Methodsmentioning
confidence: 99%
“…(Bagul et al, ) depicted that the phytic acid present in the tamarind seed might inhibit the digestive enzymes in human by forming a phytate‐protein and phytate‐mineral‐protein complexes. Also esterases (belongs to the group of hydrolytic enzymes) was responsible for the degradation of seed reserves such as xyloglucan (Kantharaju & Siddalinga Murthy, ). So the enzymes and low molecular weight compounds were inactivated by suspending in ethanol (purity 99.8%) in a ratio of 1:3 (seeds: ethanol) at 70°C for 15 min.…”
Section: Methodsmentioning
confidence: 99%
“…At 60°C, about 60% of acetic acid esterase activity was decreased . Esterases of Tamarindus indica seed were even labile at temperatures below 40°C (Kantharaju and Murthy, 2014). Thus, pearl millet FAE was comparatively more thermostable.…”
Section: Molecular Weightmentioning
confidence: 98%
“…According to from Jatropha curcas seeds to be 8.0 for isoform JEA and 7.5 for the isoform JEB. Many of the plant esterases show optimum pH between 7 and 8 (Chandrashekharaiah et al, 2011;Bhavith et al, 2014;Kantharaju and Murthy, 2014). The pH of water extract of fresh and stored pearl millet flour varies from 6.67 to 6.25 (Goyal et al, 2015).…”
Section: Molecular Weightmentioning
confidence: 99%
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“…Carboxylesterases (EC 3.1.1.1) extracted from plants could react with OPs and CAs and play an important role in the rapid determination of OP and CA residues (Gershater and Edwards, 2007;Li et al, 2009;Hou et al, 2012;Kantharaju and Murthy, 2014) because of their low cost, easy extraction, convenient preservation, sensitivity, and accuracy (Li et al, 2009). In this study, the sensitivity to various concentrations of standard pesticides, both OPs and CAs (0.001-3.0 µg/ml), was investigated by carboxylesterase enzyme inhibition assay.…”
Section: Sensitivity To Standard Ops and Cas Pesticidesmentioning
confidence: 99%