Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy

Sheng, Guo‐Ping; Yu, Han‐Qing

doi:10.1016/j.watres.2006.01.023

Cited by 663 publications

(278 citation statements)

References 18 publications

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“…The spectra were recorded at a scan rate of 12,000 nm/min, using excitation and emission slit bandwidths of 10 nm. Each scan had 37 emission and 27 excitation wavelengths (Sheng and Yu, 2006).…”

Section: Eps Characterization 24231 Extraction Of Epsmentioning

confidence: 99%

Improvement of wastewater sludge dewatering performance using titanium salt coagulants (TSCs) in combination with magnetic nano-particles: Significance of titanium speciation

et al. 2017

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a b s t r a c tIn this study, the effects of chemical conditioning using titanium salt coagulants (TSCs) of different hydrolysis speciation in combination with magnetic nano-particles on dewatering performance of waste activated sludge were evaluated by means of specific resistance to filtration (SRF) and capillary suction time (CST). The morphological and extracellular polymeric substances (EPS) properties under chemical conditioning were investigated in detail to understand the reaction mechanisms involved. The results showed that the TSC with basicity of 0.5 performed better in improving sludge dewatering performance than other TSCs. Sludge floc formed by TSC 0.5 treatment was characterized by larger floc size and higher floc strength than that conditioned by other TSCs. EPS compression and densification were the major mechanisms of sludge conditioning, and TSC 0.5 had better performance in compressing EPS structure. In addition, reduction of soluble EPS concentration, especially protein-like substances contributed to improvement of sludge filterability under conditioning. Furthermore, addition of Fe 2 O 3 nanoparticles could further improve dewatering performance and decrease compressibility of sludge system by acting as skeleton builders and enhancing floc strength. The sludge particles aggregation efficiency was effectively improved with addition of nano-Fe 2 O 3 . They also were able to bind with protein-like substance in EPS component, which might contribute to promotion of sludge filterability.

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Section: Eps Characterization 24231 Extraction Of Epsmentioning

confidence: 99%

Improvement of wastewater sludge dewatering performance using titanium salt coagulants (TSCs) in combination with magnetic nano-particles: Significance of titanium speciation

et al. 2017

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“…The spectra were recorded at a scan rate of 12,000 nm/min, using excitation and emission slit bandwidths of 10 nm. Each scan had 37 emission and 27 excitation wavelengths [20]. 2.3.3.2.1.…”

Section: Eps Extraction Proceduresmentioning

confidence: 99%

“…Firstly, four fluorescent peaks could be detected in SEPS fraction: Peak A ( ex/em = 280/335)-tryptophonlike protein, Peak B ( ex/em = 225/340)-aromatic proteins, Peaks C ( ex/em = 330/410)-humic substances and Peak D ( ex/em = 275/425)-fulvic acid while BEPS only contained Peak A and Peak B [25]. Sheng and Yu demonstrated that the fluorescent intensity in EEM spectra can be used to quantify the EPS concentration when DOC of water sample was less than 10 mg/L [20]. It was observed that the fluorescent intensity of peaks A, B, C and D was improved from 75.7, 76.9, 30.3 and 48.2 to 88.3, 82.4, 75.8 and 81.9 when pH was reduced from 7 to 2.…”

Section: Impact Of Ph On Eps Properties With/without Ferrate Treatmenmentioning

confidence: 99%

Variations in distribution and composition of extracellular polymeric substances (EPS) of biological sludge under potassium ferrate conditioning: Effects of pH and ferrate dosage

Zhang

Cao

Wang

et al. 2016

Biochemical Engineering Journal

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a b s t r a c tThe effect of pH on chemical conditioning with potassium ferrate (K 2 FeO 4 ) for improving sludge filtration dewatering performance has been studied. The variation of extracellular polymeric substances (EPS) in conditioning process was investigated in detail to unravel the reaction mechanism. The results indicated that sludge dewaterability was improved by decreasing solution pH in terms of filtration rate and cake solids content. At acid conditions, protonation of EPS resulted in reduction of negative charge and densification of sludge floc. Sludge conditioning efficiency was improved with decrease in pH. Ferrate can solubilize EPS through oxidation process and also remove a portion of soluble EPS (SEPS) by charge neutralization and interfacial adsorption of hydrolyzed ferric ions, consequently compressing EPS and decreasing total extractable EPS content. In addition, when pH and potassium ferrate were 3 and 0.1 g/gTSS, the sludge filtration dewatering rate and extent reached the maximum. Deterioration of sludge dewaterability was observed as the ferrate was overdosed (>0.2 g/gTSS). This could be attributed to the release of a large amount of bound EPS (BEPS) as a result of ferrate oxidation, consequently increasing the sludge filtration resistance.

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“…More specifically, the carbohydrate, protein and humic substance contents were reduced by 48 %, 31 %, and 72 %, respectively, when compared with their contents prior to famine exposure. EPS are fundamental to the structure of microbial aggregates and the interactions between cells, and thus are expected to control the stability of microbial aggregates [16]. Once EPS are removed from the sludge surface, the outer region of the granular sludge is likely to disperse (Fig.…”

Section: 1 Changes In Eps Contents and Settleability Of The Crushmentioning

confidence: 99%

“…Once EPS are removed from the sludge surface, the outer region of the granular sludge is likely to disperse (Fig. 2c) [16], resulting in fewer attached microbial cells and more free microbial cells [21].…”

Section: 1 Changes In Eps Contents and Settleability Of The Crushmentioning

confidence: 99%

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

Wang

Jiang

Hong

et al. 2015

Chemical Engineering Journal

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Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors, Chemical Engineering Journal (2014), doi: http://dx.doi.org/10.1016/j.cej. 2014.09.065 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ABSTRACT:Granule formation frequently occurs in denitrifying phosphorus (P) removal systems.The differences between granules and flocculants, including P removal capacities, bio-physiochemical properties, and microorganism distribution and diversity, are however still poorly understood. Physical and biochemical characteristics of granules and sludge flocs were investigated through two laboratory-scale sequencing batch reactors (SBRs). One reactor was operated as a flocculent SBR and the other 2 was operated as a granular SBR, using a granule diameter of 1.92 ± 0.78 mm. Granular sludge had a higher tolerance to inhibitors (nitrite/free nitrite acids (FNAs)), and a higher percentage of P accumulating organisms (PAOs) (72 % vs. 64 % in flocs) than flocculent sludge. The granule crush tests and higher PAOII (unable to use nitrate as an electron acceptor) to PAOs ratios (over 72 %) by fluorescent in situ hybridization showed that in both reactors, glycogen accumulating organisms (GAOs) were mainly responsible for nitrate to nitrite reduction, and PAOII further reduced nitrite to nitrogen gas in association with anoxic P uptake; GAOs wash-out weakened the mutual relationship between GAOs and PAOII to some extent, which made denitrification of nitrate to nitrite inefficient and weakened subsequent anoxic P removal. GAOs existed mainly on the surface of the granules, whereas PAOs (PAOI+PAOII ) were distributed both on the surface and in the interior of the granules. Thus, GAOs had easier access to carbon sources but were at risk of suffering from exposure to FNA.

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Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy

Cited by 663 publications

References 18 publications

Improvement of wastewater sludge dewatering performance using titanium salt coagulants (TSCs) in combination with magnetic nano-particles: Significance of titanium speciation

Improvement of wastewater sludge dewatering performance using titanium salt coagulants (TSCs) in combination with magnetic nano-particles: Significance of titanium speciation

Variations in distribution and composition of extracellular polymeric substances (EPS) of biological sludge under potassium ferrate conditioning: Effects of pH and ferrate dosage

Comparison of performance, microorganism populations, and bio-physiochemical properties of granular and flocculent sludge from denitrifying phosphorus removal reactors

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