Characterization of Fusarium spp. Causing Potato Dry Rot in China and Susceptibility Evaluation of Chinese Potato Germplasm to the Pathogen

Du, Miru; Ren, Xiangyu; Sun, Qinghua; Wang, Yi; Zhang, Ruofang

doi:10.1007/s11540-012-9217-6

Cited by 68 publications

(65 citation statements)

References 20 publications

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“…These isolates might be secondary pathogens or might require specific environmental factors that were not reproduced in the laboratory to cause infection. Less abundant but definitely pathogenic were F. sambucinum, F. avenaceum, F. culmorum and F. graminearum, and these results are in accordance with those of earlier reports (Peters et al 2008b;Du et al 2012). In our study, statistical analysis performed on the four of pathogenic Fusarium spp.…”

Section: Discussionsupporting

confidence: 93%

“…1). The resulting phylogenetic relationships remained in agreement with previous studies (Du et al 2012;Kristensen et al 2005;Zhang et al 2006). The isolates of the same species were grouped together in the resulting MP tree, supporting their correct identification.…”

Section: Discussionsupporting

confidence: 86%

“…that colonize potato tubers vary depending on geographic location and time of the survey. F. sambucinum was the species most frequently isolated from potato tubers among eight species recovered in 1992-1993 in northeastern USA (Hanson et al 1996), but also more recently in China (Du et al 2012). F. oxysporum was reported to be the primary agent responsible for potato dry rot amidst 11 species in Michigan, USA (Gachango et al 2012) and South Korea (Kim and Lee 1994).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

et al. 2016

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Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum, F. solani and F. oxysporum, depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum. Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum, F. avenaceum, F. culmorum, and F. graminearum. The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

et al. 2016

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“…Kristensen et al (2005) emphasize the importance of the TEF-1α phylogenetic analysis because they have concluded that this is able to identify species of monophyletic Fusarium. According to Du et al (2012), the region TEF-1α was efficient in the classification, into four distinct groups of species, when they sequenced 41 isolates of Fusarium since the similarity indices were higher than 95%. Hsuan et al (2011) were successful in the identification of isolates of Fusarium sp., belonging to the complex Gibberella fujikuroi obtained from maize (Zea mays), rice (Oryza sativa) and sugarcane (Saccharum officinarum) in Malaysia.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Characterization of Fusarium Spp. Pathogenic to Ilex Paraguariensis

Mezzomo

Rolim

Poletto

et al. 2018

CERNE

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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF Fusarium spp. PATHOGENIC TO Ilex Paraguariensis MEZZOMO, R.; ROLIM, J. M.; POLETTO, T.; ROSENTHAL, V. C.; SAVIAN, L. G.; REINIGER, L. R. S.; MUNIZ , M. F. B.Morphological and molecular characterization of Fusarium spp. pathogenic to Ilex Paraguariensis. CERNE, v. 24, n. 3, p. 209-218, 2018. HIGHLIGHTSDue to the low initial costs and ease of employment of agrosilvopastoral systems, the cultivation of yerba-mate constitutes an alternative for small properties in the RS to the diversification of production.The isolates of Fusarium spp. were submitted to the UPGMA analysis and grouped by the similarity between the characters averages.Through the UPGMA clustering test, the thirty-nine isolates of Fusarium spp. were allocated to seven large groups.The analysis of the TEF 1-α gene region was enough to discriminate species of Fusarium spp.The reflex symptoms resulted from the interaction of Fusarium spp. isolates with yerbamate roots. ABSTRACTThe cultivation of yerba-mate (Ilex paraguariensis) has been intensified in the State of Rio Grande do Sul, as result of the valuation of the price paid to the producer. However, studies related to Fusarium pathosystem vs. yerba-mate are still insufficient, so that many producers have faced significant losses in herbal production due to the occurrence of the roots rotting. Considering the above, the objectives of this study were: a) morphologically characterize the Fusarium spp. isolates collected in sick plants of yerba-mate; b) select efficient morphological characters on separating the Fusarium spp. isolates in similarity groups; c) identify the Fusarium spp. isolates to species level through the sequencing of the genomic region of elongation factor 1-α (TEF-1α); and d) testing the pathogenicity of the Fusarium spp. isolates selected based on the morphological characterization. For such, collections for the pathogen isolates were made in five cities and the collection points were georeferenced. Thirty-nine isolates were morphologically characterized by variables as mycelial growth, sporulation, macroconidia length and width, microconid format, colonies pigmentation and formation of chlamydospore, and tested for pathogenicity by substrate inoculation. The variables used in the morphological characterization were efficient in discriminating the isolates in seven groups, especially length of macronidia associated to sporulation. Seven pathogenic isolates of Fusarium spp. were identified, also through molecular sequencing of the TEF-1α gene region. The sequencing of the TEF-1α region consolidated the identification of pathogenic isolates as F. solani and F. oxysporum. v.24 n.3 2018 MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF FUSARIUM SPP. PATHOGENIC TO Ilex Paraguariensis 210 CERNE MEZZOMO et al.

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“…A new molecular method of PCR is widely used in science experiments together with traditional morphological and physiological research methods of Fusarium spp. [9,10].…”

Section: Introductionmentioning

confidence: 99%

Using of bioinformatics and computer morphometry in study of Fusarium spp. causing potato dry rot

Zhilinskaia¹,

Bazarnova²,

Shleikin³

et al. 2016

Ukr. food j.

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Nadezhda Zhilinskaia E-mail: jilinskie@mail.ru Introduction. The purpose of research -to evaluate the possibility of using the methods of computer morphometry and bioinformatic analysis in studying of reactive changes in Fusarium spp. causing potato dry rot.Materials and methods. Tubers of "Nevski" potato with Fusarium contamination signs were selected. Three areas of the damaged tuber potatoes were studied: the epicenter of contaminated plant tissue; peripheral area of contamination; plant tissue outside of contamination epicenter. Morphological research and computer morphometry of fixed preparations of microorganisms stained by 1,0% methylene blue solution were made under magnification x 400, x 1000.Results and discussion. Macroconidium morphometric characteristics of Fusarium spp. (square, perimeter, length, number) were obtained by automatic image analyzer "Micros". The algorithm and the computer program based on morphometric analysis of Fusarium spp. macroconidium cells causing potato dry rot was created to study bioinformatic cell characteristics.The following information characteristics of fungal cells have been calculated: H -information entropy characterizing the real structural diversity; H max -the storage capacity characterizing the maximum structural time-diversity of biological system; R (%) -information redundancy coefficient characterizing the relative organization of biosystem.The Fusarium macroconidium cells have demonstrated low values of H (0,002) compared to H max (0,5). The values of R in Fusarium cells were 99,40% -99,69% thus demonstrated a high degree of orderliness and relative organization in microbial cellss. Our research proves the maximum adaptation level of Fusarium cells as parasites in biocenosis with plant cells. It should be expected that in the case of the reaction of Fusarium cells on chemical or biological preparations the value of H should increase up to values of H max , and the value of R will decrease. It's may be occured as a result of disruption structural and functional integrity of Fusarium cell system.Conclusions. The method of bioinformatic analysis of Fusarium spp. cells causing potato dry rot can be recommended as a testing system in development of new plant protecting preparations.

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Characterization of Fusarium spp. Causing Potato Dry Rot in China and Susceptibility Evaluation of Chinese Potato Germplasm to the Pathogen

Cited by 68 publications

References 20 publications

Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

Morphological and Molecular Characterization of Fusarium Spp. Pathogenic to Ilex Paraguariensis

Using of bioinformatics and computer morphometry in study of Fusarium spp. causing potato dry rot

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