Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray

Ishiwata, Hiroko; Katsuma, Susumu; Kizaki, Keiichiro; Patel, Osman V.; Nakano, Hiroko; Takahashi, Toshio; Imai, Kyoichi; Hirasawa, Akira; Shiojima, Satoshi; Ikawa, Hiroshi; Suzuki, Yasuhito; Tsujimoto, Gozoh; Izaike, Yoshiaki; Todoroki, Junichi; Hashizume, Kazuyoshi

doi:10.1002/mrd.10292

Cited by 67 publications

(64 citation statements)

References 36 publications

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“…Placental BC products, such as bPL, bPSPB and bPAG-1, have been suggested as useful markers for embryonic or placental health, pregnancy normalcy and embryonic mortality, and for the prediction of abnormal conceptus development and postpartum pathological events (Patel et al 1997, Szenci et al 1998, Tefera et al 2001. Such BC products or the relative abundances of their placental transcripts have already been used in an attempt to detect or predict conceptus abnormalities after in vitro embryo manipulations (Vasquez et al 1995, Schmidt et al 1996, Hill et al 2000, Hashizume et al 2002, Heyman et al 2002, Ishiwata et al 2003, Patel et al 2004a, Ravelich et al 2004, and results from these studies have increasingly contributed to the understanding of those abnormalities.…”

Section: Discussionmentioning

confidence: 99%

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

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Bovine conceptus development and its association with placental proteins present in maternal, foetal and neonatal plasma and foetal (amniotic and allantoic) fluids were investigated in in vivo-and in vitro-produced (IVP) concepti and newborn calves. Females were superovulated to obtain control embryos, whereas IVP embryos were derived from established in vitro procedures. Pregnant animals were slaughtered on days 90 or 180 of gestation or allowed to develop to term for the assessment of physical traits. Foetal, maternal and neonatal blood and foetal fluids were collected for the determination of bovine placental lactogen (bPL) and bovine pregnancy-specific protein B (bPSPB) concentrations. Placental transcripts for bPL and bPSPB, determined by quantitative RT-PCR, were elevated in IVP placentomes. No major physical differences were observed between groups on day 90, but concentrations of bPL and bPSPB were higher in foetal plasma and allantoic fluid of IVP concepti in day 180 pregnancies, which were correlated with larger uterine and conceptus traits. Maternal concentrations of bPL in IVP pregnancies were lower than controls during the last 8 weeks of gestation, to become similar as parturition approached. Newborn IVP calves and foetal membranes were larger and displayed higher concentrations of plasma bPL than controls (10 and 60 min after birth). Our results indicated that differential patterns of secretion of bPL and bPSPB into the maternal and foetal systems occurred at distinct stages of gestation, and these were associated with altered conceptus development after in vitro embryo manipulations, indirectly demonstrating deviations in placental function in IVP pregnancies. Reproduction (2006) 131 163-173 IntroductionWith the onset of the trophoblast apposition to the endometrium during the process of bovine placenta formation and development, large granulated cells, the trophoblast binucleate cells (BCs), migrate from the trophectoderm to fuse with uterine epithelial cells (Wooding 1992), forming short-lived trinucleate cells. This phenomenon occurs throughout pregnancy in cattle, but the number and period of existence of trinucleate cells decline towards the end of gestation (Wooding 1992, Green et al. 2000. Binucleate cells comprise about 20% of the trophoblast cells and are responsible for hormones and bioactive products important for the regulation of foetal growth and development (Anthony et al. 1995, Green et al. 2000, Schlafer et al. 2000. The contents of the membranebounded secretory granules of BCs are released by exocytosis towards the maternal capillary bed, reaching the maternal blood system (Wooding 1992). Many proteins are produced by the BCs, including placental lactogen (PL) and a range of glycoproteins (Wooding 1992), such as pregnancy-specific protein B (PSPB) (Butler et al. 1982).PL lactogen is a member of the growth hormone (GH)/prolactin (PRL) gene family synthesized and stored in secretory granules of the trophoblast BCs (Byatt et al. 1992a, Wooding 1992, Gootwine 2004. Although ...

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Section: Discussionmentioning

confidence: 99%

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Bertolini¹,

Wallace²,

Anderson³

2006

Reproduction

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“…Microarray-based gene expression profiling in dairy cattle has been discussed recently (Beerda and Veerkamp, 2006) for studies on oocyte maturation (Dalbiè s-Tran and Mermillod, 2003;Vallee et al, 2005), non-regressed and regressed corpus luteum tissue (Casey et al, 2005), oviduct epithelial cell function (Bauersachs et al, 2003 and, the endometrium during the oestrous cycle , pre-implantation embryonic development (El-Halawany et al, 2004;Ushizawa et al, 2004;Sirard et al, 2005) and embryo-induced transcriptome changes of the endometrium in the pre-and peri-attachment period (Ishiwata et al, 2003;Klein et al, 2006). Briefly, the findings indicate that at the ovarian level, processes are typically controlled Functional genomics applied to dairy cow fertility by genes involved in steroid biosynthesis, oxygen radical metabolism, apoptosis, regulation of cell cycles (including DNA replication and repair), cell structure and tissue remodelling.…”

Section: Recent Transcriptomic Studies On Dairy Cow Fertilitymentioning

confidence: 99%

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

Beerda

Wyszyńska-Koko

Pas

et al. 2008

Animal

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Subfertility has negative effects for dairy farm profitability, animal welfare and sustainability of animal production. Increasing herd sizes and economic pressures restrict the amount of time that farmers can spend on counteractive management. Genetic improvement will become increasingly important to restore reproductive performance. Complementary to traditional breeding value estimation procedures, genomic selection based on genome-wide information will become more widely applied. Functional genomics, including transcriptomics (gene expression profiling), produces the information to understand the consequences of selection as it helps to unravel physiological mechanisms underlying female fertility traits. Insight into the latter is needed to develop new effective management strategies to combat subfertility. Here, the importance of functional genomics for dairy cow reproduction so far and in the near future is evaluated. Recent gene profiling studies in the field of dairy cow fertility are reviewed and new data are presented on genes that are expressed in the brains of dairy cows and that are involved in dairy cow oestrus (behaviour). Fast-developing new research areas in the field of functional genomics, such as epigenetics, RNA interference, variable copy numbers and nutrigenomics, are discussed including their promising future value for dairy cow fertility.

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“…Until now, only one approach was undertaken in the bovine species to characterize gene expression profiles in the bovine endometrium during pregnancy. Ishiwata et al (2003) So far, no study was conducted to investigate endometrial gene expression changes occurring in the endometrium during the pre-implantation period due to the presence of a conceptus in the bovine species.…”

Section: Maternal Recognition Of Pregnancy and The Pivotal Role Of Ifmentioning

confidence: 99%

2 Identification of Genes Induced by the Conceptus in the Bovine Endometrium During the Pre-Implantation Period

Klein¹,

Bauersachs²,

Ulbrich³

et al. 2006

Reprod. Fertil. Dev.

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Early embryonic development, implantation, and maintenance of a pregnancy are critically dependent on an intact embryo-maternal communication. So far, only few signals involved in this dialogue have been identified. In ruminants, interferon tau (IFN�) plays a key role in the process of maternal recognition of pregnancy by exhibiting antiluteolytic activity. Even though many experimental findings indicate a pivotal role of IFN� in the context of embryo-maternal communication in ruminants, a number of other systems may be involved. To identify genes induced in the bovine endometrium by the signaling of the embryo, a combination of subtracted cDNA libraries and cDNA array hybridization was applied. Monozygotic twin pairs (n = 5) were used as the biological model. Pregnancy was created in one twin by transferring two in vitro-produced embryos on Day 7 of the estrous cycle; the other twin received a sham embryo transfer. Pregnant and nonpregnant twins were slaughtered at Day 18; endometrial tissue samples were recovered and processed for transcriptome analysis as described (Bauersachs et al. 2005 J. Mol. Endocrinol. 34, 889-908). Screening of 4608 clones of two subtracted libraries revealed 90 different up-regulated genes and mRNAs, of which almost 50% are known to be stimulated by type I interferons. Among these interferon-stimulated genes, the ISG15 system is assumed to be of particular interest, and several components were studied in more detail using in situ hybridization. The pattern of mRNA expression suggests that modification of endometrial proteins through ISG15ylation plays a fundamental role during the pre-implantation period. A classification of the identified genes based on Gene Ontologies revealed the prevalence of genes involved in regulation of gene expression, cell communication, cell growth, cell differentiation, cell proliferation, and cell adhesion, and also the prevalence of genes with immune-related functions. These results underline the intense response of the endometrium to the presence of a conceptus, culminating in the preparation of the maternal environment for embryonic implantation. Further, for eleven selected genes the expression in the endometrium was quantified by the use of real-time RT-PCR. Overall, the results of quantitative RT-PCR and array hybridization correlated very well. To our knowledge this study provides the first holistic gene expression analysis of the bovine endometrium during the pre-implantation period. The results underline the importance of IFN� as an embryo-derived pregnancy recognition signal and depict the molecular mechanisms at the mRNA level underlying the intense embryo-maternal dialog taking place at Day 18 of gestation.

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Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray

Cited by 67 publications

References 36 publications

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Expression profile and protein levels of placental products as indirect measures of placental function in in vitro-derived bovine pregnancies

Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities

2 Identification of Genes Induced by the Conceptus in the Bovine Endometrium During the Pre-Implantation Period

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