Characterization of General Esterases from Methyl Parathion-Resistant and -Susceptible Populations of Western Corn Rootworm (Coleoptera: Chrysomelidae)

Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

“…Only one of the 11 probes was for a gene annotated as a putative detoxification enzyme (probe ST020027A20C03; Table S2; putative α‐esterase). Given the involvement of hydrolytic metabolism in resistance to both methyl‐parathion and carbaryl (Zhou et al ., ), this α‐esterase gene ( DvvEST ) was considered a potential contributor to observed increases in OP and carbamate insecticide metabolism amongst resistant D. v. virgifera . The derived protein sequence from the complete open reading frame (accession number KT210897) showed a catalytic triad associated with α‐esterases from other insect species (Fig.…”

“…qRT-PCR was used to validate differential expression of DvvEST from field populations collected from areas previously identified as being resistant to methyl-parathion (Phelps and York Counties, Nebraska) and one field population with known susceptibility to methyl-parathion (Saunders County, Nebraska). Susceptibility of all three populations was estimated using residual exposure to a diagnostic concentration of methyl-parathion coated on the inside of a 10-ml glass scintillation vial as described by Zhou et al (2003) and Chen et al (2011). For each population, 10 replications of 10 unsexed adults per vial were assayed.…”

“…Detoxification of carbamates is the major mechanism of resistance in D. v. virgifera, involving up-regulation of cytochrome P450 monoxygenase and general esterase activities (Scharf et al, 1999). Similarly, OP resistance in field populations from Phelps and York Counties, Nebraska, is correlated with increased cytosolic esterase activity (Zhou et al, 2002(Zhou et al, , 2003, and partly attributed to a single 66-kDa group II esterase purified from resistant individuals (Zhou et al, 2004(Zhou et al, , 2005. Assaying for mutations that cause resistance represents a novel tool that allows the tracking of changes in resistance allele frequencies in field populations (Riveron et al, 2014).…”

Foraging gene and xenobiotic stresses relationships inDrosophila melanogaster

“…ESTs, GSTs and P450s activities were tested with substrates that are not speci8c for a unique enzyme within a family but are able to evidence global variations in the activity of the family. All the protocols, adapted for working with a single 7y or with one abdomen in micro plates wells, were based on our experience or on previously published protocols (de Sousa et al, 1995;Nauen & Stumpf, 2002;Pasteur et al, 1997;Zhou et al, 2003).…”

Resistance management of the western corn rootworm (Diabrotica virgifera virgifera) : behavior, survival and the potential for cross resistance on Bt corn in the field, greenhouse and laboratory

“…After several years of assays with resistant and susceptible WCR larvae and adults, esterase-mediated resistance to methyl parathion was discovered (Miota et al 1998, Wright et al 2000, Zhou et al 2002, Zhou 2003. It was further determined that resistance to methyl parathion can be linked with increased Group II esterase proteins, and that a 66-kDa protein could be used as a resistance associated biochemical marker in assays for resistance monitoring (Zhou 2004, Zhou et al 2005.…”