Characterization of genes coding phenylalanine ammonia lyase and chalcone synthase in four Pogostemon cablin cultivars

Zeng, Shaohua; Ouyang, Ping; Mo, Xiaolu; Wang, Y.

doi:10.1007/s10535-015-0493-2

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…cablin accessions were introduced from Hainan Province in the 1960s [11, 12], thus the cultivar has been reclassified as the Hainan cultivar. Currently, the cultivars Shipai, Gaoyao, and Hainan are extensively used in the Chinese commercial market [13].…”

Section: Introductionmentioning

confidence: 99%

The plastid genome and its implications in barcoding specific-chemotypes of the medicinal herb Pogostemon cablin in China

et al. 2019

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Pogostemon cablin (Blanco) Benth. (Patchouli) is not only an important essential oil plant, but also a valuable medicinal plant in China. P . cablin in China can be divided into three cultivars (Shipai, Gaoyao, and Hainan) and two chemotypes (pogostone-type and patchoulol-type). The pogostone-type and patchoulol-type are, respectively, used for medicinals and perfumes. In this study, we sequenced and characterized the plastid genomes for all three Chinese cultivars and aimed to develop a chemotype-specific barcode for future quality control. The plastid genomes of P . cablin cultivars ranged from 152,461 to 152,462 bp in length and comprise 114 genes including 80 protein coding genes, 30 tRNA genes, and four rRNA genes. Phylogenetic analyses suggested that P . cablin cultivars clustered with the other two Pogostemon species with strong support. Although extremely conserved in P . cablin plastid genomes, 58 cpSSRs were filtered out among the three cultivars. One single variable locus, cpSSR, was discovered. The cpSSR genotypes successfully matched the chemotypes of Chinese patchouli, which was further supported by PCR-based Sanger sequences in more Chinese patchouli samples. The barcode developed in this study is thought to be a simple and reliable quality control method for Chinese P . cablin on the market.

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Section: Introductionmentioning

confidence: 99%

The plastid genome and its implications in barcoding specific-chemotypes of the medicinal herb Pogostemon cablin in China

et al. 2019

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“…The constituents exhibit marked activities, such as antibacterial 11 12 , anti-influenza virus 13 , anti-inflammatory 14 , cytotoxic 15 , antimutagenic 9 , anti-PAF–induced platelet aggregation 16 , insecticidal, and hepatoprotective activities 17 . Among these components, sesquiterpenoids and flavonoids are most abundant 18 . However, although some genes involved in terpenoid and flavonoid biosynthesis have been isolated and functionally characterized 18 19 , the global terpenoid and flavonoid biosynthesis pathways in P. cablin remain to be fully characterized.…”

mentioning

confidence: 99%

“…Among these components, sesquiterpenoids and flavonoids are most abundant 18 . However, although some genes involved in terpenoid and flavonoid biosynthesis have been isolated and functionally characterized 18 19 , the global terpenoid and flavonoid biosynthesis pathways in P. cablin remain to be fully characterized.…”

mentioning

confidence: 99%

Survey of the genome of Pogostemon cablin provides insights into its evolutionary history and sesquiterpenoid biosynthesis

Xiao

Deng

et al. 2016

Sci Rep

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Pogostemon cablin (Blanco) Benth. (Patchouli) is an important traditional Chinese medicinal plant that has both essential oil value and a broad range of therapeutic effects. Here we report the first de novo assembled 1.15-Gb draft genome sequence for P. cablin from next-generation sequencing technology. Our assembly, with a misassembly rate of <4 bp per 100 kb, is ~73% of the predicted genome size (1.57 Gb). Analysis of whole-genome sequences identified 3,147,333 heterozygous single-nucleotide polymorphisms and 490,407 insertions and deletions, giving an estimated heterozygosity rate of 0.274%. A comprehensive annotation pipeline indicated that repetitive sequences make up 58.55% of the assemblies, and that there are estimated 45,020 genes. Comparative genomics analysis showed that the Phrymaceae and Lamiaceae family split ~62.80 Mya, and the divergence between patchouli and sesame occurred ~52.42 Mya, implying a potentially shared recent whole-genome duplication event. Analysis of gene homologs involved in sesquiterpenoid biosynthesis showed that patchouli contains key genes involved in more sesquiterpenoid types and has more copies of genes for each sesquiterpenoid type than several other related plant species. The patchouli genome will facilitate future research on secondary metabolic pathways and their regulation as well as potential selective breeding of patchouli.

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“…Young leaves (YLs) of the two cultivars were harvested for the isolation of full‐length genes. To determine the spatiotemporal expression and phytochemical content, YLs, mature leaves (MLs), old leaves (OLs), young stems (YSs), mature stems (MSs) and old stems (OSs) were collected as described in the previous study (Zeng et al ). About 1 g of each sample was harvested and flash frozen in liquid nitrogen prior to storage at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, MEP‐ and MVA‐related genes were isolated as described in our previous study (Zeng et al ), followed by gene expression profiling of developmental leaves and stems of two P . cablin cultivars.…”

Section: Introductionmentioning

confidence: 99%

Aging and/or tissue‐specific regulation of patchoulol and pogostone in two Pogostemon cablin (Blanco) Benth. cultivars

Ouyang

Liu

Ying

et al. 2016

Physiologia Plantarum

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In Pogostemon cablin (Blanco) Benth. essential oil, patchoulol and pogostone are the two major bioactive phytochemicals while their in vivo biosynthesis remains largely unknown. In this study, seven genes of the plastidic methylerythritol 4-phosphate pathway (MEP) and three genes of the cytoplasmic mevalonate pathway (MVA) in two cultivars, HN and YN, were isolated. Gene expression and phytochemical profiles across leaves and stems at different developmental stages of the two cultivars were evaluated using quantitative reverse-transcription polymerase chain reaction and gas chromatography-mass spectrometry, respectively. Hierarchical analysis showed that the expression of MVA- and MEP-related genes was clustered similarly in the two cultivars. Phytochemical assay revealed that the contents of patchoulol in leaves and pogostone in stems were regulated in an aging-dependent manner. Pogostone was only detected in stems but not in leaves of the two cultivars. The Pearson correlation analysis suggested that several genes were presumably involved in the biosynthesis of patchoulol and pogostone. In the YN cultivar, the 1-deoxy-d-xylulose-5-phosphate reductoisomerase and isopentenyl pyrophosphate isomerase 2 genes, and 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase were positively responsible for patchoulol and pogostone biosynthesis, respectively. In the HN cultivar, 3-hydroxy-3-methylglutaryl-coenzyme A reductase and mevalonate diphosphate decarboxylase, and mevalonate kinase expression were positively associated with pogostone and patchoulol biosynthesis, respectively. The genes identified in this study are good candidates for the enhancement of patchoulol content in the leaves or pogostone content in the stems of P. cablin. Taken together, our results lay a solid foundation for better understanding of the mechanism underlying patchoulol and pogostone biosynthesis, which in turn may help to improve their content in P. cablin.

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Characterization of genes coding phenylalanine ammonia lyase and chalcone synthase in four Pogostemon cablin cultivars

Cited by 4 publications

References 20 publications

The plastid genome and its implications in barcoding specific-chemotypes of the medicinal herb Pogostemon cablin in China

The plastid genome and its implications in barcoding specific-chemotypes of the medicinal herb Pogostemon cablin in China

Survey of the genome of Pogostemon cablin provides insights into its evolutionary history and sesquiterpenoid biosynthesis

Aging and/or tissue‐specific regulation of patchoulol and pogostone in two Pogostemon cablin (Blanco) Benth. cultivars

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