Characterization of genetic alteration patterns in human esophageal squamous cell carcinoma using selected microsatellite markers spanning multiple loci

Cai, Yuyang; So, Chi Kwong; Nie, Alex; Song, Ying; Yang, Guang; Wang, Li Dong; Zhao, Xin; Kinzy, Terri Goss; Yang, Chung S.

doi:10.3892/ijo.30.5.1059

Cited by 12 publications

(15 citation statements)

References 23 publications

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“…In our study 27, 25 and 36% of 40 Iranian patients with esophageal cancer showed LOH at the D5S1384, D5S1478 and D5S1505 markers, respectively. It was suggested that LOH with less than 22% incidence for a marker could be regarded as a non-specific event (Cai et al, 2007). Thus, the deletions we observed were not random occurrences and a TSG(s) is/are probably located near the region studied.…”

Section: Discussionmentioning

confidence: 78%

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Loss of heterozygosity on chromosome 5 in Iranian esophageal cancer patients

Attaran-Bandarabadi

Ziaee

Yazdanbod³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. There is a high incidence of esophageal squamous cell carcinoma (ESCC) in Iran. Non-functionality of some tumor suppressor genes has been reported in esophageal cancer. Loss of heterozygosity on chromosome 5 has also been reported in esophageal carcinomas. We assessed loss of heterozygosity along a region of the long arm of chromosome 5 (5q), from 5q23.1 to 5q23.2, by PCR amplifying DNA fragments of tumor tissues from patients with ESCC and their corresponding normal samples. The PCR products were electrophoresed on 6% non-denaturing polyacrylamide gels, and band intensity was shown by silver staining. Of 40 patients with ESCC, 27, 25 and 36% of informative cases showed allelic losses at microsatellite markers D5S1384, D5S1478 and D5S1505, respectively. Two of the 40 patients studied had microsatellite instability at marker D5S1384. Based on the fact that loss of heterozygosity with more than 22% incidence for a specific marker cannot be regarded as a random event, we add support to previous reports concerning the presence of tumor suppressor genes in this chromosome region and that they affect esophageal cancer Loss of heterozygosity in esophageal cancer development. According to the data in NCBI UniSTS, the PCR product size of human DNA with primers of the D5S1505 marker ranges from 243 to 275 bp, containing about 20 repeats of the TAGA tetranucleotide, while the amplicon size of one allele of one of our cases was 207 bp, with about 10 repeats of the TAGA tetranucleotide, which would be the shortest sequence reported so far.

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Section: Discussionmentioning

confidence: 78%

“…It is reported that MSI occurs frequently in Barrett'sassociated EAC, but not in ESCC (Meltzer et al, 1994). Additionally, there is evidence implying an inverse relationship between LOH and MSI in other cancers (Cai et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Loss of heterozygosity on chromosome 5 in Iranian esophageal cancer patients

Attaran-Bandarabadi

Ziaee

Yazdanbod³

et al. 2011

Genet. Mol. Res.

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“…EC has a high incidence in people between 60 years and 64 years, and its incidence shows a gradual decrease in people over 70 years. In addition, EC presents a characteristic of noticeable geographic aggregation in China: High-incidence and -mortality areas quite concentrate, especially in the north of Henan Province near Taihang Mountains where the average mortality rate is 14.59/100,000, ranking fourth among various malignancies (Cai et al, 2007). Furthermore, EC has a general higher incidence in countrysides than in cities.…”

Section: Introductionmentioning

confidence: 99%

Influence of Personal Character on Quality of Life of Patients with Esophageal Cancer in North Henan Province and Influencing Factors

Lin

Mingsheng²,

Liu³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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The aim of this study was to investigate QoL (quality of life) of patients with esophageal cancer in northern Henan province, China, and to accurate evaluate and reflect the relationship between patient characteristics and QoL. In the high risk area of esophageal cancer in the north of Henan province, 735 patients with esophageal cancer were investigated. The Eysenck personality questionnaire (EPQ) and QoL were analyzed by using the questionnaire of general situation, EPQ, QLQ-C30 and QLQ-OES18. The effects of personal character on the QoL of esophageal carcinoma patients were analyzed by SPSS 11.0 software. The QoL of esophageal cancer patients in Northern Henan region was significantly affected by character. The difference between choleric and type of melancholic temperament types was significant (P<0.01), also in OESEAT, OESTA, OESCO and OESSP (P<0.05). Differences in personal character can thus influence the quality of esophageal cancer patient lives.

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“…Qualitatively, both MI in nDNA and heteroplasmic/homoplasmic mutation in mtDNA suggest the formation of new shifted DNA. Microsatellite markers, including mononucleotide poly-C repeats on mtDNA (e.g., D310) or dinucleotide CA repeat on nDNA close to TP53 (e.g., D17S960) have been analyzed in TESCC, however, their roles and associations in TESCC have remained obscure (6,(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Associated microsatellite alterations in mitochondrial DNA and in TP53 in thoracic esophageal squamous cell carcinoma

Lin¹,

Wang²,

Chang³

et al. 2012

Oncol Rep

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Abstract. We investigated the microsatellite alterations in mitochondrial DNA (mtDNA) and in TP53 in thoracic esophageal squamous cell carcinomas (TESCC). Using laser microdissection, 66 paired non-cancerous esophageal muscles, non-cancerous esophageal mucosa, cancerous TESCC nests plus 35 metastatic lymph nodes harvested from 66 resected esophagi of TESCC patients were subjected to DNA extraction. D310 and D17S960 were chosen as markers to address microsatellite alterations in mtDNA, including changes in copy number and homoplasmic/heteroplasmic mutations of mtDNA, and in TP53, including loss of heterozygosity (LOH) and microsatellite instability (MI). From non-cancerous esophageal mucosa to cancerous TESCC nests and then metastatic lymph nodes, a trend of homoplasmic D310 mutation (10.6, 25.8, 31.4%; P=0.023), an ever increase of mtDNA copy ratios (0.892, 1.128, 1.183; P=0.018) and an elevated incidence of TP53 LOH (19.7, 34.8,37.1%; P=0.010) were observed. From T1, T2, T3 to T4 TESCC, the incidence of TP53 LOH (12.5, 16.7, 34.8, 52.2%; P=0.011) was increased, in a stepwise fashion. Furthermore, we observed an association of TP53 LOH with an increased mtDNA copy ratio (P=0.022) and TP53 MI with heteroplasmic D310 mutation (P=0.069) in TESCC. Concurrent and associated microsatellite alterations in mtDNA and in TP53 in TESCC support the cancer clonal expansion theory and imply a possible relationship between the mitochondria and p53 in TESCC.

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Characterization of genetic alteration patterns in human esophageal squamous cell carcinoma using selected microsatellite markers spanning multiple loci

Cited by 12 publications

References 23 publications

Loss of heterozygosity on chromosome 5 in Iranian esophageal cancer patients

Loss of heterozygosity on chromosome 5 in Iranian esophageal cancer patients

Influence of Personal Character on Quality of Life of Patients with Esophageal Cancer in North Henan Province and Influencing Factors

Associated microsatellite alterations in mitochondrial DNA and in TP53 in thoracic esophageal squamous cell carcinoma

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