Characterization of genotypic variation in stress gene expression and photosynthetic parameters in potato

Seppänen, Mervi; Coleman, Gary D.

doi:10.1046/j.1365-3040.2003.00971.x

Cited by 13 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The ability of the plants to minimize the oxidative stress depends upon the effectiveness of the antioxidants, which has been reported to vary according to the plant species and their genotypes (Blokhina et al, 2003;Seppanen and Coleman, 2003). From these results it is clear that AGB002 showed superior expression of antioxidant enzymes on exposure to cold stress than ABG025.…”

Section: Antioxidant Enzymesmentioning

confidence: 93%

“…The ability of the plants to minimize oxidative stress depends upon the effectiveness of enzymatic and non-enzymatic antioxidants (Blokhina et al, 2003;Seppanen and Coleman, 2003). The antioxidant potential is highly variable in different plant species and in different genotypes of the same species.…”

Section: Introductionmentioning

confidence: 99%

“…Stress tolerant genotypes have often been reported to show higher expression of antioxidants as reported for salt (Walia et al, 2005), drought (Türkan et al, 2005), cold (Seppanen and Coleman, 2003,b;Kumar et al, 2008a,b;Mutlu et al, 2013) and heat stress (Snider et al, 2010;Almeselmani et al, 2009). The responsiveness and efficiency of scavenging systems of plants governs their performance, endurance, and survival capability under stress conditions (Seppanen and Coleman, 2003;Mishra et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

View full text Add to dashboard Cite

Withania somnifera (L.) Dunal (Indian ginseng) is a high value medicinal plant. It synthesizes a large array of biologically active withanolides. In this study, two month old seedlings of AGB002 (wild genotype) and AGB025 (cultivated genotype) of W. somnifera were subjected to cold stress (4 • C) under controlled envi-ronment. Plants were analyzed for three medicinally important secondary metabolites (withanolide A, withanone and withaferin A), lipid peroxidation (MDA), cell injury, superoxide radical (O 2 •− ) accumula-tion and anti-oxidative enzymes activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Increases in the titers of superoxide anion and MDA were observed from day 1 to day 7 in both genotypes, although the increase on the first day of exposure was significantly higher. Enzymatic activities of SOD, CAT, APX and GR also showed an increasing trend in both genotypes and reached a maximum on day 7 of the cold temperature exposure; however, this increase was higher in AGB002 than AGB025. Withanolide A (WS-1) in the roots of both genotypes sig-nificantly decreased on the first day of cold exposure and then showed a recovery until day 7. WS-1 was not detected in the leaves of either genotype. Withanone (WS-2) content in the leaves also decreased towards the end of the cold period in both genotypes. Cold stress also elicited the accumulation of WS-2 in AGB025, but was not detectable in control seedlings. At maturity, WS-2 was also detected in control plants. Furthermore, a significant increase in the leaf withaferin A (WS-3) content was recorded from day 1 to day 7 of the cold exposure in both the genotypes, suggesting the possible involvement of with-anolides in cold-protection. AGB002 showed comparatively higher accumulation of antioxidant enzymes and selected marker withanolides than AGB025, indicating that AGB002 is better adapted to cold than AGB025. It could be inferred from these observations that cold stress induces bioactive withanolide accu-mulation in W. somnifera as a mechanism for scavenging reactive oxygen species (ROS). These studies also provide an impetus for enhancing the withanolide accumulation in W. somnifera using controlled environment technology.

show abstract

Section: Antioxidant Enzymesmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

View full text Add to dashboard Cite

show abstract

“…Studies with a potato population derived from a self-pollinated somatic hybrid revealed that antioxidative capacity plays an important role in frost tolerance. Various genes responding to low temperatures or associated with accumulation of active oxygen species at low temperature and intensive light were identified Fagerstedt 2000, Seppänen andColeman 2003). These studies were continued to increase understanding of the genetic and physiological basis of the nonacclimated freezing tolerance in potato.…”

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

View full text Add to dashboard Cite

Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

show abstract

“…The environmental sensitivity of photosynthesis has allowed it to be used to screening for genetic differences in stress tolerance in several species (Fracheboud et al , Sharma et al , Zhou et al ). In potato, however, there are only few studies and little is known about the photosynthetic capacity of potato under different types of environmental stresses (Seppänen and Coleman , Hassanpana , M'Hamdi et al , Anithakumari et al ). Other studies report only small or even no differences between genotypes of potato for photosynthetic traits (Havaux and Davaud , Balemi and Schenk ).…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato

Prinzenberg

Víquez-Zamora²,

Harbinson

et al. 2018

Physiologia Plantarum

View full text Add to dashboard Cite

Physiology and genetics are tightly interrelated. Understanding the genetic basis of a physiological trait such as the quantum yield of the photosystem II, or photosynthetic responses to environmental changes will benefit the understanding of these processes. By means of chlorophyll fluorescence (CF) imaging, the quantum yield of photosystem II can be determined rapidly, precisely and non-invasively. In this article, the genetic control and variation in the steady-state quantum yield of PSII (Φ PSII ) is analyzed for diploid potato plants. Current progress in potato research and breeding is slow due to high levels of heterozygosity and complexity of tetraploid genetics. Diploid potatoes offer the possibility of overcoming this problem and advance research for one of the globally most important staple foods. With the help of a diploid genetic mapping population two genetic loci that were strongly associated with differences in Φ PSII were identified. This is a proof of principle that genetic analysis for Φ PSII can be done on potato. The effects of three different stress conditions that are important in potato cultivation were also tested: salt stress, low temperature and deficiency in the macronutrient phosphate. For the last two stresses, significant decreases in photosynthetic activity could be shown, revealing potential for stress detection with CF based tools. In general, our findings show the potential of high-throughput phenotyping for physiological research and breeding in potato.

show abstract

Characterization of genotypic variation in stress gene expression and photosynthetic parameters in potato

Cited by 13 publications

References 53 publications

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato

Contact Info

Product

Resources

About