Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: I. evaluation of the GTI QuikID assay and analysis of antibody patterns

Delaney, Nancy; Lucas, Donna P.; Leffell, Mary S.

doi:10.1016/s0198-8859(00)00254-8

Cited by 39 publications

(24 citation statements)

References 7 publications

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“…We have observed that certain antigens tend to yield weaker or stronger results than others. It is difficult to determine from these observations whether the difference is in the stability of the target, the avidity of antibody, or the immunogenicity of different antigens [18]. Additionally, reactivity with some or all antigens or phenotypes can vary from lot to lot.…”

Section: Discussionmentioning

confidence: 99%

“…The titer of CDC assays was given as the inverse of the highest dilution yielding a positive crossmatch. Flow cytometric crossmatches were performed as described previously [18]. The results of flow cytometric crossmatches were expressed either as the ratio of the median channel fluorescence of the test serum to that of the negative control (FCXM ratio) or as molecules of equivalent soluble fluorochrome (MESF).…”

Section: Crossmatch Testsmentioning

confidence: 99%

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Using real data for a virtual crossmatch

et al. 2009

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Section: Discussionmentioning

confidence: 99%

Section: Crossmatch Testsmentioning

confidence: 99%

Using real data for a virtual crossmatch

et al. 2009

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“…The multianalyte immunoassays were performed according to manufacturer's instructions as reported elsewhere (12)(13)(14).…”

Section: Donor-specific Antibodymentioning

confidence: 99%

The Utility of Splenectomy as Rescue Treatment for Severe Acute Antibody Mediated Rejection

Locke

Zachary

Haas

et al. 2007

American Journal of Transplantation

123

103

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Antibody-mediated rejection (AMR) after desensitization for a positive crossmatch (+XM) live donor renal transplant can be severe and result in sudden onset oliguria and loss of the allograft. Attempts to rescue these kidneys using plasmapheresis (PP) and IVIg may be ineffective due to the magnitude of antibody burden that must be controlled to prevent renal thrombosis or cortical necrosis. We review our experience using splenectomy combined with PP/IVIg as rescue therapy for patients experiencing an acute deterioration in renal function and a rise in donor-specific antibody within the first posttransplant week after desensitization for a +XM. Five patients underwent immediate splenectomy followed by PP/IVIg and had return of allograft function within 48 h of the procedure. Emergent splenectomy followed by PP/IVIg may be an effective treatment for reversing severe AMR.

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“…We demonstrated that the sensitivity and specificity of these assays were greatly increased over that of cytotoxicity assays and, in our hands, had a sensitivity comparable to that of cell-based flow cytometry. Another advantage of these ELISAs is that they avoided many of the technical and logistical problems of cell-based assays [20]. We had expected that there would be an increased frequency of all specificities.…”

Section: Technology Research and Developmentmentioning

confidence: 99%

Immunogenetics and immunology in transplantation

2010

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Advances in immunogenetics and histocompatibility have facilitated the clinical transplantation of solid organs and tissues. Improved definition of HLA antigens, alleles, and haplotypes has clarified the diversity of the HLA system among different racial/ethnic populations. Knowledge of allele and haplotypes frequencies derived from these studies can be applied to the estimates of transplant compatibility and the likelihood finding suitable donors. Humoral sensitization that has been a major barrier to transplantation is being successfully treated with desensitization and paired donor exchanges. Sensitive solid phase immunoassays for HLA-specific antibodies permit analyses that guide both desensitization and donor exchanges through antibody monitoring, virtual crossmatches, and detection of cryptic sensitization. Ongoing studies are investigating the mechanisms underlying the down-regulation of donor HLA-specific antibodies.

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Characterization of HLA class I specific antibodies by ELISA using solubilized antigen targets: I. evaluation of the GTI QuikID assay and analysis of antibody patterns

Cited by 39 publications

References 7 publications

Using real data for a virtual crossmatch

Using real data for a virtual crossmatch

The Utility of Splenectomy as Rescue Treatment for Severe Acute Antibody Mediated Rejection

Immunogenetics and immunology in transplantation

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