Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Thamm, Christopher; DeSimone, Elise; Scheibel, Thomas

doi:10.1002/mabi.201700141

Cited by 11 publications

(1 citation statement)

References 62 publications

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“…[28,29] eADF4(C16) can be produced recombinantly at large scale as well as processed into a variety of morphologies other than fibres [30] such as films for coatings [27,[31][32][33][34][35] or soft hydrogels for biofabrication. [36][37][38] In this context, it has been shown to be a promising material for coating of polyurethane, polytetrafluoroethylene or silicone-based materials. [29] Materials made of eADF4(C16) are perfectly suited for biomedical applications since they display the absence of toxicity, lack of immune reactivity and slow biodegradation.…”

Section: Introductionmentioning

confidence: 99%

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

et al. 2021

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Bacterial infections are well recognised to be one of the most important current public health problems. Inhibiting adhesion of microbes on biomaterials is one approach for preventing inflammation. Coatings made of recombinant spider silk proteins based on the consensus sequence of Araneus diadematus dragline silk fibroin 4 have previously shown microbe-repellent properties. Concerning silicone implants, it has been further shown that spider silk coatings are effective in lowering the risk of capsular fibrosis. Here, microbial repellence tests using four opportunistic infection-related strains revealed additional insights into the microbe-repellent properties of spider silk-coated implants, exemplarily shown for silicone surfaces. Graphic Abstract

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Section: Introductionmentioning

confidence: 99%

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

et al. 2021

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Artificial Spider Silk Materials: From Molecular Design, Mesoscopic Assembly, to Macroscopic Performances

Shi,

Zhu,

Qian

et al. 2024

Adv Funct Materials

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Spider silk is one of the strongest and toughest fibrous materials available in nature. Its superior properties make it a good candidate for applications in various fields ranging from protective body armor to scaffolds for medical implants. However, harvesting a substantial amount of natural spider silk remains challenging because spiders cannot be easily bred. With the development of synthetic biology and its integration with materials science, considerable research has been directed toward engineering and production of synthetic spider silk materials. Here the study overviews general strategies on molecular design, mesoscopic assembly, and macroscopic regulation of artificial spider silk materials. The insights into the correlation between silk protein sequences, mesoscopic assemblies, and macroscopic material properties are provided for guiding de novo design and engineering of next‐generation spider silk materials. This review also emphasizes the challenges and future perspectives for advancing the translational research on these designer functional materials for diverse applications.

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Rapidly Forming Recombinant Miniature Spidroins Hydrogels Composed of Nanofibrils with Tunable Mechanical Properties for Bio 3D Printing and Biomimetic Cellular Scaffolds

Wang,

Zhang,

et al. 2024

Adv Funct Materials

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Recombinant spidroins offer numerous possibilities for creating new biomaterials. However, their polymorphic and prone to aggregation characteristics present challenges in both their production and practical application. Here, mutant recombinant spidroins are reported forming hydrogels rapidly and controllably at 37 °C and with visible light irradiation. In the mutant spidroins phenylalanine residues (F) are systematically substituted by tyrosine residues (Y) in repeat motifs of (GGX), which contributes to the self‐assembly of β‐sheet and further formation of amyloid‐like nanofibrils. As expected, micellar/globular spidroins solution converts to spidroins hydrogel composed of nanofibrils network and subsequently further crosslinked by di‐tyrosine. The conformation transformation process is verified by spectroscopy, transmission electron microscopy (TEM), and molecular dynamics simulation. Furthermore, the spidroin hydrogels are used as bioink and biomimetic cellular scaffolds according to their good biocompatibility, shear thinning properties, and nanofibril network structure. The findings reveal the structural transformation mechanism of spidroins and expand their applications in biomedical engineering.

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Characterization of Hydrogels Made of a Novel Spider Silk Protein eMaSp1s and Evaluation for 3D Printing

Cited by 11 publications

References 62 publications

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Microbial repellence properties of engineered spider silk coatings prevent biofilm formation of opportunistic bacterial strains

Artificial Spider Silk Materials: From Molecular Design, Mesoscopic Assembly, to Macroscopic Performances

Rapidly Forming Recombinant Miniature Spidroins Hydrogels Composed of Nanofibrils with Tunable Mechanical Properties for Bio 3D Printing and Biomimetic Cellular Scaffolds

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