Characterization of Staphylococcus aureus Isolates from Buffalo, Bovine, Ovine, and Caprine Milk Samples Collected in Rio de Janeiro State, Brazil

Abstract

Eighty-four staphylococcal isolates were obtained from milk samples from cows, sheep, goats, and buffalo with subclinical mastitis and from colonization samples from ostriches. The animals were hosted in 18 small dairy herds and an ostrich breeding located in 10 municipalities of the state of Rio de Janeiro, Brazil. Thirty isolates were identified as Staphylococcus aureus by biochemical and molecular techniques and were comparatively characterized by phenotypic and genotypic methods. The molecular characterization by pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing (MLST) revealed five clonal types (PFGE A, spa type t359, sequence type 747 [ST747]; PFGE B, spa type t1180, ST750; PFGE C, spa type t605, ST126; PFGE D, spa type t127, ST751; and PFGE F, spa type t002, ST5). None of the isolates harbored the Panton-Valentine leukocidin or exfoliative toxin D gene. The detection of major clone A (in 63% of the isolates) in different herds, among all animal species studied, and in infection and colonization samples evidenced its geographical spread among Rio de Janeiro State and no host preference among the animal species. Comparison with S. aureus from a human origin suggested that all but one clone found in the present study might be animal specific.

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

“…The same fact is reported by Aires-de-Sousa et al (2007) comparing the sensitivity of Staphylococcus aureus isolates from bovine, goats and sheep.…”

“…isolates (7.1%). Although the antimicrobial resistance observed in the present study seems to be higher than those previously reported in Brazil and elsewhere (Aires-de-Sousa et al 2007, Kumar et al 2009), direct comparisons must be performed carefully since most investigations focused only on Staphylococcus aureus. Except for enroϐloxain and norϐloxacin, the present results indicate lower resistance rates to streptomycin, tetracycline and amoxicillin in Staphylococcus aureus as compared to CNS (Table 2).…”

“…Staphylococci are considered to be the most important mastitis causing agents in ruminants (Aires-de-Sousa et al 2007) and antimicrobial therapy is usually required in treatment (Bergonier et al 2000, Contreras et al 2007, Pÿorälä & Taponen 2009). However, there is evidence that resistance to antibiotics is increasing in staphylococci infected food animals (Phillips et al 2004).…”

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

“…Though the 88 S. aureus isolated between 2010 and 2012 belong to 14 different pulsotypes, 3 of them (A, B, D) group together 72 % and 5 of them (A, B, D, N, Z), 83 % of the isolates, confirming, like in other studies, that a restricted number of S. aureus clones are present within a herd or a farm (Hata et al 2006;Aires-de-Sousa et al 2007;Rabello et al 2007;Anderson et al 2012). Nevertheless, apart from the A and B pulsotypes (>90 % similarity), the other pulsotypes are not closely related and represent distinct lineages (Fig.…”

Mastite em pequenos ruminantes no Brasil

“…No Brasil, os estudos com epidemiologia molecular, envolvendo S. aureus isolados de caprinos e ovinos ainda são escassos (Aires-de-Sousa et al 2007). Estes autores caracterizaram S. aureus isolados de casos de mastite subclínica nas espécies bovina, bubalina, caprina e ovina de diferentes municípios do estado do Rio de Janeiro.…”