Characterization of Kewda volatile components by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry

Abstract

Kewda (Pandanus fascicularis Lam.) is a well known medicinal and aromatic plant. The paper aims to precisely characterize volatile constituents present in Kewda flower oil using comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry (GCxGC-TOFMS). A total of 159 components were identified due to enhanced chromatographic separation and mass spectral deconvolution of GCxGC-TOFMS. On the basis of its chemical structure, the identified compounds were grouped into hydrocarbons, alcohols, ethers, ketones, esters, nitrogen compounds, aldehydes, acids, lactones, halides and sulfur containing compounds. Ethers were the major components. The predominant compounds identified by GCxGC-TOFMS were kewda ether, ortho-cymene and terpinen-4-ol. A database containing retention indices of compounds was created for the bi-dimensional column, thus proving to be a remarkable step for analysis of constituents using a GCxGC system. GCxGC-TOFMS separated a number of co-eluting components which were unresolved on a single GC column.

Plant-derived natural products for drug discovery: current approaches and prospects

“…Mass spectrometric (MS) fingerprints also can be developed. Chromatographic fingerprints can be obtained using Thin-layer chromatography (TLC) [ 77 ], High-performance thin-layer chromatography (HPTLC) [ 18 ], High performance liquid chromatography (HPLC) [ 17 ], Ultra-high performance liquid chromatography (UHPLC) [ 105 ], Capillary electrophoresis (CE) [ 29 ], Gas chromatography (GC) [ 64 ], Gas chromatography-mass spectrometry (GC–MS) [ 61 ], Two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) [ 63 ].…”

“…It has been used widely by many workers to analyze various phytoconstituents because of its high efficiency, reproducibility, sensitive detection, simplicity and stability [ 61 ], GC × GC-TOFMS is the most efficient separation tool for analyzing complex mixtures due to its high resolution and high peak capacity. Using two columns with varying separation methodology makes this technique more advantageous by increasing resolution, sensitivity, and identification of more unknown compounds [ 63 ]. This technique can be used to detect minor components, develop comprehensive fingerprints and detect unknown volatile constituents of the herbal drug.…”

Metabolite profiling of Piper longum L. fruit volatiles by two‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity

“…However, the signal‐to‐noise ratio threshold can be modified case to case. In an earlier study in Pandanus fascicularis and Alpinia galanga , it was set to greater than 50 and greater than 300, respectively 27,25 . Peaks with a similarity value lower than 800 were excluded from the selection, as they did not show a good probability of matching.…”

“…24 Thus, for precise separation and detection, GC×GC-TOFMS is more advanced than GC-MS. Essential oils from Curcuma zedoaria, Curcuma angustifolia, Alpinia galanga, Hedychium coronarium, Nicotiana tabacum, Pandanus fascicularis, and Piper betle were analyzed by GC×GC-TOFMS, which revealed more constituents than GC-MS. 9,17,25,26,27,28 Despite possessing enormous therapeutic and economical significance, P. longum lacks in-depth chemical characterization. The characterization of fruit volatile oil of long pepper has been reported in several reports by 1D GC-MS, indicating the presence of around 50 constituents.…”

“…Thus, for precise separation and detection, GC×GC–TOFMS is more advanced than GC–MS. Essential oils from Curcuma zedoaria , Curcuma angustifolia , Alpinia galanga , Hedychium coronarium , Nicotiana tabacum , Pandanus fascicularis , and Piper betle were analyzed by GC×GC–TOFMS, which revealed more constituents than GC–MS 9,17,25,26,27,28 …”