Characterization of KPC-encoding plasmids from two endemic settings, Greece and Italy

Papagiannitsis, Costas C.; Pilato, Vincenzo Di; Giani, Tommaso; Giakkoupi, Panagiota; Riccobono, Eleonora; Landini, Giulia; Miriagou, Vivì; Vatopoulos, Alkiviadis; Rossolini, Gian María

doi:10.1093/jac/dkw227

Cited by 42 publications

(44 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other rearrangements of the pKpQIL backbone have also been reported elsewhere (12, 13, 16), indicating the dynamic genetic nature of this plasmid and suggesting that these variants may be the result of selection for adaptation to the host in a niche environment.…”

Section: Introductionsupporting

confidence: 70%

“…Although the bla KPC carbapenemase can be found in various Enterobacteriaceae (22), it is predominantly associated with K. pneumoniae and the pKpQIL plasmid (7, 24). This plasmid has a high propensity for genetic rearrangements (12, 13, 16), but the benefit of such genetic plasticity and consequences of evolution of the plasmid in relation to different hosts remain unclear. Here, we investigated whether the pKpQIL-D2 plasmid confers a different fitness impact to that of the more widely observed pKpQIL-UK plasmid.…”

Section: Discussionmentioning

confidence: 99%

“…As with many other regions around the world (12, 16), KPC-producing K. pneumoniae have been isolated in the UK and pKpQIL-UK (which differs from the original Israel pKpQIL by a few base substitutions) has been identified in major hospital outbreaks of carbapenem-resistant K. pneumoniae in the country (17, 18). In one UK region, both pKpQIL-UK and a variant where a 19.5 kb region in the former has been substituted with a 17.6 kb fragment have been found to be in circulation in isolates from patients (17).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Different impacts of naturally occurring variants of the globally disseminated carbapenemase-encoding plasmid pKpQIL on the biology of host strains

Saw

Webber

Woodford

et al. 2018

Preprint

View full text Add to dashboard Cite

2 Synopsis (240 words) 19Klebsiella-associated plasmid pKpQIL and its variant have been isolated globally. Our 20 study aimed to determine whether a naturally occurring variant has altered host range 21 and impacts on the fitness of different bacterial host strains. The plasmids pKpQIL-UK 22 and pKpQIL-D2 were transferred from the original clinical isolate host strains of Klebsiella 23 pneumoniae into Escherichia coli, Salmonella Typhimurium, Enterobacter cloacae and 24 Serratia marcescens strains by filter-mating and conjugation frequencies determined and 25 compared. The fitness of the resulting transconjugants was assessed by determining 26 growth kinetics, ability to form a biofilm and persistence of the plasmids in each host was 27 also measured. Transfer of either plasmid into Salmonella and S. marcescens was 28 similar. However, pKpQIL-UK transferred into E. coli at a higher rate than did pKpQIL-D2; 29 the reverse was found for E. cloacae. Both plasmids were rapidly lost from the E. coli 30 population. Plasmid pKpQIL-UK, but not -D2, was able to persist in Salmonella. Although 31 pKpQIL-UK imposed a greater fitness cost (inferred from an increased generation time) 32 than -D2 on E. cloacae, it was able to persist as well as pKpQIL-D2 in this host. The 33 pKpQIL-D2 plasmid did not confer any fitness benefit on any of the hosts under the 34 conditions tested. Variants of the globally important pKpQIL plasmid have arisen in 35 patients due to recombination. The impacts of the pKpQIL-UK plasmid and the -D2 variant 36 in various Enterobacteriaceae are host-dependent. Continuing evolution of pKpQIL may 37 alter its host range in the future. 38 39 40 370 This project was supported by the Elite Doctoral Researcher Scholarship of the University

show abstract

Section: Introductionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Different impacts of naturally occurring variants of the globally disseminated carbapenemase-encoding plasmid pKpQIL on the biology of host strains

Saw

Webber

Woodford

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…pBK30661 was identified as an IncFIA plasmid harboring nine ARGs for β‐lactam resistance ( bla KPC‐3 , bla TEM‐1 , bla OXA‐9 ), aminoglycoside resistance ( aacA4 , aadA1 , strA , strB ), sulfonamide resistance ( sul2 ), and trimethoprim resistance ( dfrA14 ) . Other IncF types, such as pKP1504‐KPC and pGR‐1780, have also been reported to spread bla KPC‐2 in K. pneumoniae clinical isolates, specifically isolates ST258 and ST147 …”

Section: Plasmid Biology and Incompatibility Groupsmentioning

confidence: 99%

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Kopotsa

Sekyere

Mbelle

2019

Annals of the New York Academy of Sciences

179

178

View full text Add to dashboard Cite

Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities. Resistance to multiple β‐lactams complicates effective clinical management of CRE infections. Using plasmid typing methods, a wide distribution of plasmid replicon groups has been reported in CREs around the world, including IncF, N, X, A/C, L/M, R, P, H, I, and W. We performed a literature search for English research papers, published between 2013 and 2018, reporting on plasmid‐mediated carbapenem resistance. A rise in both carbapenemase types and associated plasmid replicon groups was seen, with China, Canada, and the United States recording a higher increase than other countries. blaKPC was the most prevalent, except in Angola and the Czech Republic, where OXA‐181 (n = 50, 88%) and OXA‐48–like (n = 24, 44%) carbapenemases were most prevalent, respectively; blaKPC‐2/3 accounted for 70% (n = 956) of all reported carbapenemases. IncF plasmids were found to be responsible for disseminating different antibiotic resistance genes worldwide, accounting for almost 40% (n = 254) of plasmid‐borne carbapenemases. blaCTX‐M, blaTEM, blaSHV, blaOXA‐1/9, qnr, and aac‐(6′)‐lb were mostly detected concurrently with carbapenemases. Most reported plasmids were conjugative but not present in multiple countries or species, suggesting limited interspecies and interboundary transmission of a common plasmid. A major limitation to effective characterization of plasmid evolution was the use of PCR‐based instead of whole‐plasmid sequencing–based plasmid typing.

show abstract

“…To our knowledge, this is the first report on complete sequences of IncR plasmids carrying Tn4401a transposon. However, previous studies have reported the presence of multireplicon IncFII K2 -IncR KPC-2-encoding plasmids from ST101 K. pneumoniae isolated in Italian hospitals (31,32). In addition, type B plasmids were derivatives of type A IncR bla KPC-2positive plasmids carrying an IncN3-derived segment.…”

mentioning

confidence: 93%

Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Kukla

Chudějová

Papagiannitsis

et al. 2018

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Ten isolates collected in a Czech hospital carried-positive plasmids of different sizes (∼30, ∼45, and ∼80 kb). Sequencing revealed three types of plasmids (A to C) with the Tn transposon. Type A plasmids comprised an IncR backbone and a KPC-2-encoding multidrug resistance (MDR) region. Type B plasmids were derivatives of type A plasmids carrying an IncN3-like segment, while type C plasmids were IncP6 plasmids sharing the same KPC-2-encoding MDR region with type A and B plasmids.

show abstract

Characterization of KPC-encoding plasmids from two endemic settings, Greece and Italy

Abstract: pKpQIL-like plasmids played a major role in the dissemination of blaKPC in Greece and Italy, but evolved with different dynamics in these endemic settings.

Cited by 42 publications

References 50 publications

Different impacts of naturally occurring variants of the globally disseminated carbapenemase-encoding plasmid pKpQIL on the biology of host strains

Different impacts of naturally occurring variants of the globally disseminated carbapenemase-encoding plasmid pKpQIL on the biology of host strains

Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review

Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital

Contact Info

Product

Resources

About