Characterization of Listeria monocytogenes isolated from Ganges water, human clinical and milk samples at Varanasi, India

Soni, Dharmendra Kumar; Singh, Rakesh Kumar; Singh, Deepika; Dubey, Suresh Kumar

doi:10.1016/j.meegid.2012.09.019

Cited by 71 publications

(62 citation statements)

References 43 publications

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“…They observed that 17 of 149 human clinical samples screened from 2006 to 2009 were positive for L. monocytogenes, of which 15 (88%) isolates were positive for L. monocytogenes serotype 4b. In a recent study, Soni et al (2013) examined L. monocytogenes isolated from the Ganges River, and clinical human specimens and milk samples from Varanasi, India. They observed that isolates from humans and water belonged to 4b, 4d, 4e or 1/2c, 3c serogroups, while milkborne isolates belonged to serogroups 1/2b, 3b or 1/2a, 3a.…”

Section: Resultsmentioning

confidence: 99%

Multi-Virulence-Locus Sequence Typing of 4bListeria monocytogenesIsolates Obtained from Different Sources in India over a 10-Year Period

Doijad

Lomonaco

Poharkar

et al. 2014

Foodborne Pathogens and Disease

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Listeria monocytogenes is an emerging foodborne pathogen responsible for listeriosis. The incidence of listeriosis has increased during the last 2 decades due to the increase in consumption of ready-to-eat foods and change in food consumption habits. Outbreaks and sporadic cases of listeriosis have been reported in developed countries. These reports have helped determine the safety practices needed to control listeriosis. Although L. monocytogenes has been reported from humans, animals, and a variety of foods in India, limited data exist with respect to prevalence and distribution of L. monocytogenes in the Indian subcontinent. The Indian Listeria Culture Collection Centre in Goa maintains all of the isolates received for subtyping and molecular characterization. Of the listerial isolate collection maintained by this center, three fourths of the isolates are of 4b serotype, while the number of other serotypes is very low. Therefore, we screened L. monocytogenes serotype 4b isolates to determine their relevance to previously defined epidemics and/or outbreaks using multi-virulencelocus sequence typing (MVLST). A total of 25 isolates in serogroup 4b of L. monocytogenes were randomly selected from a repository of 156 L. monocytogenes 4b isolates obtained from different sources in India over a period of 10 years. MVLST sequence types (virulence types, VTs) were compared to known epidemic clones and other known isolates in the L. monocytogenes MVLST database. The 25 isolates were grouped into three clusters. Cluster I comprised 21 isolates including animal (n = 9), human (n = 4), and food (n = 8), which matched Epidemic Clone I (ECI, VT20). Three isolates-two from animal and one from food-formed a cluster while a single animal isolate was placed into two novel VTs (VT98 and VT99), respectively. Based on these findings, it can be inferred that ECI has been isolated from a variety of sources and places and has persisted in India for at least 10 years.

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Section: Resultsmentioning

confidence: 99%

Multi-Virulence-Locus Sequence Typing of 4bListeria monocytogenesIsolates Obtained from Different Sources in India over a 10-Year Period

Doijad

Lomonaco

Poharkar

et al. 2014

Foodborne Pathogens and Disease

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“…There are very scarce reports on listeriosis from Indian human population. However, L. monocytogenes was isolated (0.3%) of 633, (1.31%) of 305 and (1.7%) of 300 samples from patients with poor obstetric history of abortions, miscarriages, stillbirths or neonatal deaths as reported by Dhanashree et al (2003); Kaur et al (2007); Soni et al (2013), respectively. The findings of the present study on the prevalence of L. monocytogenes in human clinical samples are similar to the earlier reports 0.3% to 14% range in pregnant women.…”

Section: Discussionmentioning

confidence: 99%

“…Human clinical samples (HPb1-12 and HVS1-18) used in this study from our laboratory stock collection were examined following the standard double enrichment method of ISO 11290:1 with slight modifications (Anonymous, 1997;Soni et al, 2013). Further, identification of the pathogen was confirmed by biochemical and molecular characterizations were used as controls.…”

Section: Identification Of L Monocytogenesmentioning

confidence: 99%

“…The PCR products were analyzed on agarose gel (1.5%), stained with ethidium bromide, and visualized under UV transilluminator (AlphaImager EC). The details of oligonucleotide sequences (Sigma) and PCR cyclic conditions used in this study are given elsewhere (Soni et al, 2013.…”

Section: Species-and Virulence-specific Genes and Serogroup Identificmentioning

confidence: 99%

“…The amplicons were electrophoresed in 1.8% agarose gel at 60 V (6 h), stained with ethidium bromide and analyzed as described earlier (Soni et al, 2013. The fingerprint pattern was measured in a Fluoro-S-Imager (Bio-Rad) and analyzed using Bionumerics fingerprint analyst (Applied Maths) software with a simple-matching similarity matrix, and the data clustered by the un-weighted pair group method with arithmetic means (UPGMA).…”

Section: Eric-and Rep-pcr Based Genomic Fingerprintingmentioning

confidence: 99%

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Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples

2015

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Listeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. The present study aimed at characterizing L. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and ERIC- and REP-PCR fingerprint analyses. The results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from vaginal swab (2200)] were positive for L. monocytogenes. All the isolates belonged to serogroup 4b, and were + ve for virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA, hlyA, and iap. Based on the mice inoculation tests, 20 isolates showed 100% and 4 isolates 60% relative virulence while 6 isolates were non-pathogenic. Moreover, 2 and 10 isolates were resistant to ciprofloxacin and cefoxitin, respectively, while the rest susceptible to other antibiotics used in this study. ERIC- and REP-PCR collectively depicted that the isolates from placental bit and vaginal swab had distinct PCR fingerprints except a few isolates with identical patterns. This study demonstrates prevalence of pathogenic strains mostly resistant to cefoxitin and/or ciprofloxacin. The results indicate the importance of isolating and characterizing the pathogen from human clinical samples as the pre-requisite for accurate epidemiological investigations.

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Listeria and Erysipelothrix

Smith,

Harris

2023

ClinMicroNow

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Listeria and Erysipelothrix are genera of small, non‐sporeforming, Gram‐positive bacilli that include the opportunistic human pathogens Listeria monocytogenes and Erysipelothrix rhusiopathiae . The clinical presentation of an individual exposed to Listeria varies from asymptomatic to mild gastroenteritis to life‐threatening illness, depending on a combination of factors that include bacterial virulence, inoculum size, and underlying host defenses. Isolation of Listeria can be achieved from clinical specimens from normally sterile sites, including blood and cerebrospinal fluid. Typing of Erysipelothrix is used for the analysis of outbreaks in farmed animals and other epidemiologic purposes but has little clinical relevance in humans. Historically, strain typing was performed by serologic testing based on somatic and flagellar antigens. Due to the lack of resistant strains, breakpoints exist for susceptible results only.

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Characterization of Listeria monocytogenes isolated from Ganges water, human clinical and milk samples at Varanasi, India

Cited by 71 publications

References 43 publications

Multi-Virulence-Locus Sequence Typing of 4bListeria monocytogenesIsolates Obtained from Different Sources in India over a 10-Year Period

Multi-Virulence-Locus Sequence Typing of 4bListeria monocytogenesIsolates Obtained from Different Sources in India over a 10-Year Period

Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples

Listeria and Erysipelothrix

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