Characterization of Low‐virulent Mouse Coronavirus Isolated from Faeces in a Mouse Colony

Hirasawa, Tsutomu; Hirano, Norio; Ohhara, Sayoko; Mikazuki, Katsumi; Hayashi, Yuriko

doi:10.1111/j.1439-0450.1988.tb00517.x

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…MHV strain 1 (MHV-1), MHV-2, MHV-3, MHV-JHM, MHV-A59, and MHV-S are prototype strains. MHV-D, MHV-DVIM, MHV-F-2D, MHV-IN-12, MHV-N, MHV-Nu, MHV-NuA, and MHV-NuU are lowvirulence isolates Hirasawa et al, 1988). MHV-2-CC, MHV-JHM-CC, and MHV-A59-CC are low-virulence mutants of the respective strains 2, JHM, and A59 (Hirano et al, 1984).…”

Section: Viruses and Cellsmentioning

confidence: 98%

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

Hanaki

Ike

Hatakeyama

et al. 2013

Journal of Virological Methods

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Mouse hepatitis virus (MHV) is one of the most prevalent viruses detected in laboratory mouse colonies. Enterotropic strains predominate in natural infections, and molecular techniques for the detection of MHV shedding in feces are powerful enough to diagnose active infections. A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technique was developed for the detection of rodent coronaviruses within 90 min. The specificity of this technique was confirmed by its ability to detect all 17 different strains of MHV and 6 strains of rat coronaviruses as well as its failure to detect human, bovine, and porcine coronaviruses nonspecifically. The sensitivity of RT-LAMP was 3.2-fold higher than that of reverse transcription-polymerase chain reaction (RT-PCR) and 31.6-fold lower than that of nested RT-PCR. An evaluation of the diagnostic performance of RT-LAMP performed in duplicate using mouse fecal specimens showed that the sensitivity and specificity with respect to nested RT-PCR were 85.7% and 100%, respectively. RT-LAMP assays would be suitable for monitoring active MHV infection in mouse colonies.

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Section: Viruses and Cellsmentioning

confidence: 98%

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

Hanaki

Ike

Hatakeyama

et al. 2013

Journal of Virological Methods

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Natural Infection of Nude Mice with Low‐virulent Mouse Coronavirus

Hirano

Ono

1990

Journal of Veterinary Medicine, Series B

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Summary The outbreak of wasting disease of nude mice occurred in the laboratory colony of a Pharmaceutical Company. The viruses producing cytopathic effect with syncytium formation were isolated from the wasted nude mice by DBT cells, and were identified as mouse coronavirus by direct immunofluorescence. The nude mouse colony was closed and all the nude mice (about 500) were killed by the reason of disease control. At autopsy about 60 % of nude mice showed necrotic hepatitis. By the virus isolation to see the source of contamination, viruses were isolated from the feces of apparently healthy mice of ICR, CDF1, DBA/2 and C3H, and from human cancer cell line stocked in liquid nitrogen. In experimental infection, the isolates produced only mild hepatitis in ICR mice treated with cortisone. By cross‐neutralization test, the nude isolate reacted closely with the virus from C3H mice but not with the virus from cancer cell line. The isolates from nude and C3H mice produced experimentally wasting disease with necrotic hepatitis in nude mice. These findings suggest that wasting disease in nude mice might be caused by low‐virulent mouse coronavirus shed in feces from C3H mice introduced before the outbreak of disease.

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Characterization of Low‐virulent Mouse Coronavirus Isolated from Faeces in a Mouse Colony

Cited by 2 publications

References 15 publications

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

Natural Infection of Nude Mice with Low‐virulent Mouse Coronavirus

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