Characterization of LrpC DNA-Binding Properties and Regulation of
            <i>Bacillus subtilis lrpC</i>
            Gene Expression

Beloin, Christophe; Exley, Rachel M.; Mahé, Anne-Laure; Zouine, Mohamed; Cubasch, Stephanie; Hégarat, Françoise Le

doi:10.1128/jb.182.16.4414-4424.2000

Cited by 25 publications

(22 citation statements)

References 51 publications

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“…This result is not surprising considering the sequenceindependent binding properties of H-NS. Both Lrp and H-NS have been shown to have multiple binding sites around promoters and form nucleoprotein structures which influence transcription (Soutourina et al 1999;Beloin et al 2000). Lrp and H-NS may therefore form a complex that influences traJ transcription.…”

Section: Discussionmentioning

confidence: 98%

H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100

et al. 2003

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Conjugative transfer of F-like plasmids is a tightly regulated process. The TraJ protein is the main positive activator of the tra operon which encodes products required for conjugative transfer of F-like plasmids. Nucleotide sequence analysis revealed potential Lrp and H-NS binding sites in the traJ regulatory region. Expression of a traJ-lacZ fusion in hns and lrp mutant strains showed that both are positive modulators of traJ expression. Competitive RT-PCR demonstrated that H-NS and Lrp exert their effect at the transcriptional level. Electrophoretic mobility-shift assays showed that H-NS and Lrp proteins bind to the traJ promoter. Conjugative transfer of pRK100 was decreased in hns but not in lrp mutant strains. Together, the results indicate H-NS and Lrp function as activators of traJ transcription.

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Section: Discussionmentioning

confidence: 98%

H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100

et al. 2003

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“…The 15 basepair SELEX-derived consensus sequence (C/T)AG(A/C/T)A(A/T)ATT(A/T)T(A/G/T)CT(A/G) is very degenerate but has helped in the identification of Lrp binding sites within bacterial promoters (Cui et al 1995). This study and others have demonstrated that E. coli Lrp and certain homologues prefer binding to regions that are intrinsically curved, consisting of multiple A/T basepairs (Beloin et al 2000;Tapias et al 2000;Peterson et al 2007). The SELEX method selected for short specific sequences that were favourably bound by one Lrp dimer.…”

Section: Role In Dna Organizationmentioning

confidence: 83%

“…Various Lrp consensus sequences have been identified. The Lrp homologue LrpC from Bacillus subtilis has a greater binding affinity for a curved region 5¢ of the lrpC gene that did not contain the SELEX-selected consensus compared to a region where the consensus was present (Beloin et al 2000). In the pap operon the consensus sequence for the six Lrp binding sites in the regulatory region is GN 2-3 TTT (Nou et al 1995).…”

Section: Role In Dna Organizationmentioning

confidence: 99%

Bacterial Chromatin

2010

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this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.Cover illustration: H-NS-DNA complex visualized using scanning force microscopy (Courtesy of R.T. Dame)Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) v PrefaceThe birth and the development of molecular biology and, subsequently, of genetic engineering and biotechnology cannot be separated from the advancements in our knowledge of the genetics, biochemistry and physiology of bacteria and bacteriophages. Also most of the tools employed nowadays by biotechnologists are of bacterial (or bacteriophage) origin and the playground for most of the DNA manipulations still remains within bacteria. The relative simplicity of the bacterial cell, the short generation times, the well defined and inexpensive culturing conditions which characterize bacteria and the auto-catalytic process whereby a wealth of in-depth information has been accumulated throughout the years have significantly contributed to generate a large number of knowledge-based, reliable and exploitable biological systems.The subtle relationships between phages and their hosts have produced a large amount of information and allowed the identification and characterization of a number of components which play essential roles in fundamental biological processes such as DNA duplication, recombination, transcription and translation. For instance, to remain within the topic of this book, two important players in the organization of the nucleoid, FIS and IHF, have been discovered in this way. Indeed, it is difficult to find a single fundamental biological process whose structural and functional aspects are better known than in bacteria.However, a notable exception is represented by the physical and functional organization of the bacterial genome. Although some bacteria contain more than one chromosome and some chromosomes are known to be linear, the majority of bacterial cells contain a single circular chromosome. The chromosome of Escherichia coli consists of about 4.6 million bp corresponding to a fully extended circumference of about 1.6 mm and rapidly growing bacteria may contain up to almost four genomic equivalents. Thus, the need for compaction of this genetic material to fit within an approximately 500-fold smaller volume is obvious; likewise, also clear is the need for a dynamic "chromatin" structure capable of undergoing rapidly all kinds of vital transactions to respond promptly to different types of environmental cues, changes and stresses with focused and/or global reprogramming of gene expression. All this happens within one or a few ill-defined structures called "nucleoids" where the cellular DNA is localized.The ...

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“…These suggest that Lrp plays an indispensable role in various metabolic events. Unlike most Lrp/AsnC family member, B. subtilis LrpC is positively autoregulated and mainly expressed in stationary growth phase [Beloin et al, 2000] impervious to leucine [Lopez-Torrejon et al, 2006].…”

Section: The Expression Regulation Of Lrp/asnc Genesmentioning

confidence: 99%

Regulatory and pathogenesis roles of Mycobacterium Lrp/AsnC family transcriptional factors

2011

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Lrp/AsnC (leucine-responsive regulatory protein/asparagine synthase C products) family transcriptional regulators, widespread among bacteria and archaea, is also known as feast/famine regulatory protein (FFRPs). They regulate multiple cellular metabolisms globally (Lrp) or specifically (AsnC), such as amino acid metabolism, pili synthesis, DNA transactions during DNA repair and recombination, and also might be implicated in persistence. To better understanding of the pathogenesis of M. tuberculosis, based on our lab's work on this transcriptional factor family, these progresses are summarized, with special focus on that of Mycobacterium via comparative genomics.

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Characterization of LrpC DNA-Binding Properties and Regulation of Bacillus subtilis lrpC Gene Expression

Cited by 25 publications

References 51 publications

H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100

H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100

Bacterial Chromatin

Regulatory and pathogenesis roles of Mycobacterium Lrp/AsnC family transcriptional factors

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