Characterization of Lung Hyperresponsiveness, Inflammation, and Alveolar Macrophage Mediator Production in Allergy Resistant and Susceptible Rats

Careau, Eric; Sirois, Jocelyne; Bissonnette, Élyse

doi:10.1165/ajrcmb.26.5.4737

Cited by 33 publications

(34 citation statements)

References 30 publications

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“…The transfer of AM from Sprague Dawley rats to AM-depleted Brown Norway rats abrogated both the early phase reaction and airway hyperresponsiveness development, suggesting an important role for these cells in preventing asthma symptoms. We have also demonstrated that OVA sensitization without challenge differentially influences AM cytokine production (TNF, IL-10, IL-12, and IL-13) in Brown Norway and Sprague Dawley rats (8), suggesting that OVA sensitization might differently modulate AM functions in both strains of rats. In the present study, we characterized airway responsiveness and inflammation as well as AM cytokine production and phagocytosis in sensitized and unsensitized Brown Norway rats after OVA challenge.…”

Section: Discussionmentioning

confidence: 70%

“…We have previously demonstrated that Brown Norway rats, a widely used asthma model, develop airway inflammation and hyperresponsiveness after allergen sensitization and challenge in contrast to allergy-resistant Sprague Dawley rats (7,8). The transfer of AM from Sprague Dawley rats to AM-depleted Brown Norway rats abrogated both the early phase reaction and airway hyperresponsiveness development, suggesting an important role for these cells in preventing asthma symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…Airway responsiveness was measured 24 h after OVA challenge as previously described (8). Briefly, animals were anaesthetized, intubated, and connected to a Quatra-T CombiChamber (Scireq, Montreal, QC, Canada) to measure lung resistance (R L).…”

Section: Methodsmentioning

confidence: 99%

“…Alveolar macrophages (AM) are predominant immune effector cells in the alveolar spaces and conducting airways and play a key role in the immunological homeostasis of the lung (8,30,43). Although they are well known to suppress T cell activation and antigen presentation activities of dendritic cells (22,41), their role in asthma is still debated.…”

mentioning

confidence: 99%

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Antigen sensitization modulates alveolar macrophage functions in an asthma model

Careau¹,

Proulx²,

Pouliot³

et al. 2006

American Journal of Physiology-Lung Cellular and Molecular Phys

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-We have previously demonstrated that adoptive transfer of alveolar macrophages from allergy-resistant rats to alveolar macrophage-depleted allergic rats prevents airway hyperresponsiveness development, suggesting an important role for alveolar macrophages in asthma pathogenesis. Given that ovalbumin sensitization can modulate alveolar macrophage cytokine production, we investigated the role of sensitized and unsensitized alveolar macrophages in an asthma model. Alveolar macrophages from unsensitized or sensitized Brown Norway rats were transferred to alveolar macrophage-depleted sensitized rats 24 h before allergen challenge. Airway responsiveness to methacholine and airway inflammation were measured the following day. Methacholine concentration needed to increase lung resistance by 200% was significantly higher in alveolar macrophage-depleted sensitized rats that received unsensitized alveolar macrophages compared with alveolar macrophage-depleted sensitized rats that received sensitized alveolar macrophages. Tumor necrosis factor levels in bronchoalveolar lavage fluid of sensitized rats that received unsensitized alveolar macrophages were significantly lower compared with rats that received sensitized alveolar macrophages. Interestingly, alveolar macrophages of unsensitized animals showed higher phagocytosis activity compared with alveolar macrophages of sensitized rats, suggesting that sensitization modulates alveolar macrophage phagocytosis function. Our data suggest an important role of allergen sensitization on alveolar macrophage function in asthma pathogenesis. airway hyperresponsiveness; cytokine; phagocytosis THE INCIDENCE OF allergic respiratory diseases increased dramatically over the last two decades. The genetic predisposition to develop asthma is now well recognized (20). Numerous studies have also shown the importance of inflammatory cells, such as mast cells, lymphocytes, eosinophils, and neutrophils, as well as Th2 type cytokines in asthma (6,19,32). Interleukin (IL)-4 and IL-13, two Th2 cytokines, are important in immunoglobulin (Ig) class switching to IgE, whereas IL-4 and IL-10 play a crucial role in Th2 cell commitment (9,16,50). In contrast, Th1 cytokines (interferon-␥ and IL-12) protect against asthma development in children (48). Other cytokines, such as tumor necrosis factor (TNF), have been demonstrated to amplify the effect of asthmatic inflammation (29,42). Recently, a role of regulatory T cells (Th3 cells, T R cells, CD4 ϩ CD25 ϩ cells, and natural killer T cells) has been suggested in the control of asthma and allergy, but the specific mechanisms are still unknown (2).Alveolar macrophages (AM) are predominant immune effector cells in the alveolar spaces and conducting airways and play a key role in the immunological homeostasis of the lung (8, 30, 43). Although they are well known to suppress T cell activation and antigen presentation activities of dendritic cells (22,41), their role in asthma is still debated. Using the liposome-mediated macrophage suicide technique (47), we have...

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Antigen sensitization modulates alveolar macrophage functions in an asthma model

Careau¹,

Proulx²,

Pouliot³

et al. 2006

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Esta resposta via Th2 é 33 mediada pela produção de EROs e citocinas como: IL-4, IL-5 e IL-13. As alterações que caracterizam a asma alérgica são: elevada IgE sérica, intensa secreção de muco, eosinofilia e exacerbada reatividade brônquica (MATTES et al, 2002;HYLKEMA et al, 2002;CAREAU et al, 2002 (ALI et al, 1999;FRANGOGIANNIS et al, 2001;KUBES, 2002 (DAHLEN et al, 1981;FORTES et al, 1991;FARSKY et al, 1995FARSKY et al, , 2004.…”

Section: Hidroquinonaunclassified

Estudo dos mecanismos de ação da hidroquinona e fenol sobre o recrutamento leucocitário em respostas inflamatórias

Macedo¹

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The role of macrophage IL‐10/innate IFN interplay during virus‐induced asthma

et al. 2014

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Activation through different signaling pathways results in two functionally different types of macrophages, the pro-inflammatory (M1) and the anti-inflammatory (M2). The polarization of macrophages toward the pro-inflammatory M1 phenotype is considered to be critical for efficient antiviral immune responses in the lung.Among the various cell types that are present in the asthmatic airways, macrophages have emerged as significant participants in disease pathogenesis, because of their activation during both the inflammatory and resolution phases, with an impact on disease progression. Polarized M1 and M2 macrophages are able to reversibly undergo functional redifferentiation into anti-inflammatory or pro-inflammatory macrophages, respectively, and therefore, macrophages mediate both processes.Recent studies have indicated a predominance of M2 macrophages in asthmatic airways. During a virus infection, it is likely that M2 macrophages would secrete higher amounts of the suppressor cytokine IL-10, and less innate IFNs. However, the interactions between IL-10 and innate IFNs during virus-induced exacerbations of asthma have not been well studied.The possible role of IL-10 as a therapy in allergic asthma has already been suggested, but the divergent roles of this suppressor molecule in the antiviral immune response raise concerns. This review attempts to shed light on macrophage IL-10–IFNs interactions and discusses the role of IL-10 in virus-induced asthma exacerbations. Whereas IL-10 is important in terminating pro-inflammatory and antiviral immune responses, the presence of this immune regulatory cytokine at the beginning of virus infection could impair the response to viruses and play a role in virus-induced asthma exacerbations.

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Characterization of Lung Hyperresponsiveness, Inflammation, and Alveolar Macrophage Mediator Production in Allergy Resistant and Susceptible Rats

Cited by 33 publications

References 30 publications

Antigen sensitization modulates alveolar macrophage functions in an asthma model

Antigen sensitization modulates alveolar macrophage functions in an asthma model

Estudo dos mecanismos de ação da hidroquinona e fenol sobre o recrutamento leucocitário em respostas inflamatórias

The role of macrophage IL‐10/innate IFN interplay during virus‐induced asthma

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