Characterization of multiple absorbed constituents in rats after oral administration of Paederia scandens decoction

Abstract

Paederia scandens (Lour.) Merri. (Jishiteng in Chinese) is a Chinese traditional medicine widely used in treating various diseases. However, its active components have remained unknown. In the present study, a rapid and sensitive method by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-MSn) techniques was employed to investigate the absorbed constituents in rats after oral administration of Paederia scandens decoction. By comparing their MS data with those of authentic compounds and published data, a total of six compounds (paederosid, 1; paederosidic acid, 2; paederosidic acid methyl ester, 3; 6-hydroxy geniposide, 4; asperuloside, 5; and deacetyl asperuloside, 6) were identified in the P. scandens decoction samples. In addition, a total of seven compounds, including three iridoid glucosides and four of their metabolites, were identified in rat urine samples after administration. In addition, six compounds, including four iridoid glucosides and two of their metabolites, were identified in rat serum samples after administration. Our results significantly narrow the range of potentially active compounds in P. scandens decoction, and build a solid foundation for future research on its mechanism.

Novel insights into the fruit and seed extracts ofMorinda morindoides(Baker) Milne‐Redh: HPLC‐ESI‐Q‐TOF‐MS profiling, antioxidant, and enzyme inhibitory propensities

“…Compound 8 was tentatively described as a saccharide derivative based on bibliographic information (Spínola, Pinto, & Castilho, 2015). Compound 11 was characterized as asperuloside based on its molecular formula and previously reported fragmentation (Jiang et al, 2012). Compound 13 was characterized as esculetin based on its molecular formula and base peak at m/z 111, produced by the loss of 44 Da (CO 2 moiety) (Zhou et al, 2008).…”

Hepatoprotection of Paederia scandens (Lour.) Merr. on Acetaminophen-Related Hepatic Injury Rats by 1H-NMR-Based Metabonomics Coupled with Network Pharmacology

“…Similar protective effects were observed in silymarin-treated rats (Figure 1(e)). 1 H-NMR Spectra. 1 H-NMR analysis was performed on serum samples of control, model, and PSM-treated groups representing the normal and pathological states and protective effects, respectively.…”

“…Merr. (PSM), a traditional Chinese medicine also used as a vegetable, is a member of the “generally recognized as safe” (GRAS) category of plants used for therapeutic purposes [ 1 , 2 ]. The tender stems and leaves of PSM and Hainanese “chicken excrement fragrant cake” made with PSM are consumed as local traditional food.…”

Identification of the absorptive constituents and their metabolites in vivo of Ziziphi Spinosae Semen by UPLC–ESI–Q‐TOF–MS/MS

“…Nowadays, ultra‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry (UPLC–ESI–Q–TOF–MS/MS) has been widely used for qualitative and quantitative analysis of complex sample matrices, e.g. biological samples and traditional Chinese medicine extracts (Jiang et al, 2012; Xie, Zhong, & Chen, 2011; Xie, Zhong, Yu, & Chen, 2012; Zhao et al, 2013; Zhao et al, 2015; Zhou et al, 2013). In contrast with conventional high‐performance liquid chromatography (HPLC), ultra‐performance liquid chromatography (UPLC) shows higher peak efficiency and chromatographic resolution, owing to smaller column particles with a diameter of 1.8 μm.…”