Characterization of natural hydroxyapatite originated from fish bone and its biocompatibility with osteoblasts

Shi, Peipei; Li, Meng; Fan, Fan; Yu, Chang Yeon; Lu, Weihong; Du, Ming

doi:10.1016/j.msec.2018.04.026

Cited by 166 publications

(103 citation statements)

References 40 publications

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“…The EDS spectra showed that peak intensities corresponding to Ca and P of the HAP particles dramatically decreased after nano‐milling, indicating the exchange of components between the HAP particles and the suspension during the milling process (Supova, ; Yin et al, ). Furthermore, Ca/P atomic ratios values of the nanosized HAP samples (0.33–0.62) were significantly lower than the microsized HAP sample (1.63), which was related to the release of calcium to the suspension during the milling process (Shi et al, ; Yin et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…The patterns shown in Figure b–f corresponding to the HAP, collagen/HAP and amino acids/HAP samples with 240 min of nano‐milling, respectively, predominately exhibited one sharp diffraction peak at 25.8° and one broad peak ranging from 30 to 35°. With respect to the HAP sample without nano‐milling, peak numbers of the five HAP samples with nano‐milling were much less and their diffraction intensities obviously lower, suggesting the reduction of the crystallinity of HAP by nano‐milling (Shi et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…(a) hydroxyapatite (HAP), (b) HAP, (c) collagen/HAP, (d) glycine/HAP, (e) glutamic acid/HAP, (f) tyrosine/HAP F I G U R E 3 EDS spectra of hydroxyapatite (HAP) samples without nano-milling (a) and with 240 min of nano-milling (b-f). (a) hydroxyapatite (HAP), (b) HAP, (c) collagen/HAP, (d) glycine/HAP, (e) glutamic acid/HAP, (f) tyrosine/HAPcalcium to the suspension during the milling process(Shi et al, 2018;Yin et al, 2015).…”

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confidence: 99%

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Mechanism on releasing and solubilizing of fish bone calcium during nano‐milling

Yin

Xiong

et al. 2019

J Food Process Engineering

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Fish bone, predominately composited by collagen and hydroxyapatite (HAP), is a natural calcium resource that possesses the potential to be used as functional ingredient in food application. In this study, five HAP suspensions with either collagen or amino acids were applied to simulate the releasing and solubilizing of fish bone calcium during nano‐milling. As milling time was increased, particle sizes from five suspensions gradually decreased and pH increased. The sizes and pH kept constant as the time was extended beyond 60 min (p < .05). Initial soluble calcium content of HAP suspension fluctuated with milling time and was not increased after milling for 240 min. However, soluble calcium contents of HAP suspensions with collagen and amino acids continuously increased and reached the highest values of 106.70 and 147.34–645.68 μg/g, respectively. After nano‐milling, crystallinity of HAP particles declined and calcium element on the surface decreased and contents of free amino acid and peptides from fish bone suspension significantly increased (p < .05). Our results suggested that the releasing and solubilizing of fish bone calcium during nano‐milling was mainly attributed to alterations on size and crystalline structure of HAP, and the chelating effects by amino acids and peptides. Practical Applications Fish bone is the main solid waste from fish processing industry. When properly processed, it possesses the potential to be used as food grade calcium additive. It has been reported that grittiness of fish bone could be neglected after reducing its particle to nanoscale. Furthermore, soluble calcium content was significantly increased, which might be benefit for improving calcium bioavailability of fish bone. However, there is no report regarding the release of calcium from fish bone during nano‐milling process so far. This research will provide scientific foundation for improving solubility of calcium from various natural resources in a physical method.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Mechanism on releasing and solubilizing of fish bone calcium during nano‐milling

Yin

Xiong

et al. 2019

J Food Process Engineering

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“…FTIR peak at 3637.88 cm -1 indicated the free O-H stretching vibration [7]. The 961.65 cm -1 peak indicated stretched modes of phosphate [17] while the 656.01 cm -1 peak indicated metal oxide stretching vibration [18]. Furthermore, it could be observed that the peak at 715.65 cm -1 was totally removed which indicate a complete elimination of C-H organic substances at the temperature of 800°C.…”

Section: Fig 2 Ftir Spectra Of Untreated and Furnace Heated Shellsmentioning

confidence: 90%

Scylla Serrata Shells Calcination using Electric Furnace and Microwave Kiln

Hii¹,

Nor²,

Kurniawan³

2019

IJEAT

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To accommodate the rising demands for artificial bone substitutes, the possibilities of extracting calcium oxide (CaO) as precursor of hydroxyapatite (HAP) from Scylla serrata (crab) shells via electric furnace and microwave kiln heating (calcination) were investigated. The crab shells were obtained from food wastes from local restaurants in Miri, Malaysia. They were treated by calcination with temperatures of 600°C, 800°C, and 1000°C for 3 and 4 hours in electric furnace. The shells were also calcined in microwave kiln at power levels of 60%, 80% and 100% for 1 hour. Characterization on the calcined shell samples were done using Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Diffractometer (XRD). Based on the FTIR and XRD spectra, it was found that calcium oxide was obtained through calcination at temperatures ≥ 800°C. The results also indicated that a higher calcination temperature and duration would yield CaO with larger crystallite size. Through calcination in microwave kiln, CaO was readily produced when calcination was done at 60% power.

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“…Attempts have been made to isolate fish bone derived hydroxyapatite and use it as an alternate for synthetic hydroxyapatite (Kim et al, 1997;Ozawa and Suguru, 2002). For example, Shi et al (2018) reported that among natural hydroxyapatite derived from rainbow trout, cod, and salmon bones, the ones from rainbow trout and salmon bones led to a faster growth, adhesion, differentiation, and proliferation of osteoblast and formation of mineralized tissue which is most likely attributed to the presence of CO 3 2− and Mg 2+ . Generally, heat treatment (800∼1,300 °C) is used for the isolation of hydroxyapatite from fish bone and high temperature gives high strength and higher degree of crystallinity and larger crystallite size to hydroxyapatite structure Pal et al, 2017;Sunil and Jagannatham, 2016) and provides an excellent biocompatible inorganic substance Venkatesan et al, 2015).…”

Section: Application Of Fish Bone Minerals In Biomedical Industrymentioning

confidence: 99%

Utilization of marine by-products for the recovery of value-added products

Shahidi¹,

Varatharajan²,

Han³

et al. 2019

Journal of Food Bioactives

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The world fisheries resources have exceeded 160 million tons in recent years. However, every year a considerable amount of total catch is discarded as by-catch or as processing leftovers, and that includes trimmings, fins, frames, heads, skin, viscera and among others. In addition, a large quantity of processing by-products is accumulated as shells of crustaceans and shellfish from marine bioprocessing plants. Recognition of the limited marine resources and the increasing environmental pollution has emphasized the need for better utilization of the by-products. Marine by-products contain valuable protein and lipid fractions, minerals, enzymes as well as many other components. The major fraction of by-products are used for feed production-in making fish meal/oil, but this has low profitability. However, there are many ways in which the fish and shellfish waste could be better utilized, including the production of novel food ingredients, nutraceuticals, pharmaceuticals, biomedical materials, fine chemicals, and other value-added products. In recent times, much research is conducted in order to explore the possible uses of different by-products. This contribution primarily covers the characteristics and utilization of the main ingredients such as protein, lipid, chitin and its derivatives, enzymes, carotenoids, and minerals originating from marine by-products.

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Characterization of natural hydroxyapatite originated from fish bone and its biocompatibility with osteoblasts

Cited by 166 publications

References 40 publications

Mechanism on releasing and solubilizing of fish bone calcium during nano‐milling

Mechanism on releasing and solubilizing of fish bone calcium during nano‐milling

Scylla Serrata Shells Calcination using Electric Furnace and Microwave Kiln

Utilization of marine by-products for the recovery of value-added products

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