Characterization of Necrotizing Lymphadenitis Associated with Porcine Circovirus Type 2 Infection

Galindo-Cardiel, I.; Grau‐Roma, Llorenç; Pérez-Maíllo, Monica; Segalés, Joaquím

doi:10.1016/j.jcpa.2010.06.003

Cited by 16 publications

(13 citation statements)

References 20 publications

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“…48 Though initially suspected, 48 apoptosis or programmed cell death was later found not to be involved in lymphocyte depletion in cases with PCV2-associated necrotizing lymphadenitis. 28 Microscopic lesions in lymphoid tissues of fetuses, if present, are very similar to those observed in growing pigs: Lymph nodal and splenic lymphocyte depletion with occasional multinucleated giant cells 11,61,65 or lymph node follicular hyperplasia 10 have been described.…”

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confidence: 85%

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Opriessnig

Langohr

2012

Vet Pathol

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Porcine circovirus type 2 (PCV2), a small single-stranded DNA virus, was initially discovered in 1998 and is highly prevalent in the domestic pig population. Disease manifestations associated with PCV2 include postweaning multisystemic wasting disease (PMWS), enteric disease, respiratory disease, porcine dermatitis and nephropathy syndrome (PDNS), and reproductive failure. Although these clinical manifestations involve different organ systems, there is considerable overlap in clinical expression of disease and presence of lesions between pigs and within herds. It is now widely accepted that PCV2 can be further subdivided into different types, of which PCV2a and PCV2b are present worldwide and of greatest importance. This review will focus on PCV2-associated lesions in different organ systems.

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confidence: 85%

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Opriessnig

Langohr

2012

Vet Pathol

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“…Cirkowirus świń typu 2 jest czynnikiem infekcyjnym wywołującym szereg zaburzeń u świń (2), obejmują-cych poodsadzeniowy wielonarządowy zespół wyniszczający (PMWS), a także zaburzenia oddechowe, zapalenie mięśnia sercowego i inne, określane ogólnie jako choroby związane z PCV2 (PCVD) (7,19,21,31,66,67,77). W ostatnim czasie pojawia się coraz więcej doniesień na temat wpływu PCV2 na rozród trzody chlewnej -prace opisujące masowe ronienia i inne zaburzenia w rozrodzie powiązane z zakażeniem PCV2 (56).…”

Section: Cirkowirus śWiń Typuunclassified

Infectious agents involved in reproduction failure in swine

Kwit¹,

Pomorska‐Mól²,

Markowska-Daniel³

2016

Medycyna Weterynaryjna

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“…As described above, the blockage of the PI3K/Akt pathway by LY294002 enhanced PCV2-induced apoptotic responses. In order to further investigate the role of the PI3K/Akt pathway in the cell survival after PCV2 infection, PK15 cells were preincubated for 1 h with increasing concentrations (10,15,20, and 40 M) of the pancaspase inhibitor zVAD.fmk in either the absence or the presence of LY294002 (20 M). Cells were then infected with PCV2 at an MOI of 1 TCID 50 , and at 1 h postinfection, cell lysates were collected and subjected to Western blotting with anti-phospho-Akt antibody.…”

Section: Indirect Immunofluorescence Assay (Ifa)mentioning

confidence: 99%

“…PCV2 infection triggers apoptosis both in vitro and in vivo (7,20,26,31,32,44,48,53). It has been further demonstrated that PCV2 infection induces apoptosis via activating the caspase-8 followed by the caspase-3 pathway (32).…”

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confidence: 98%

Activation of the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway during Porcine Circovirus Type 2 Infection Facilitates Cell Survival and Viral Replication

Zhu

Wang

et al. 2012

J Virol

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Virus infection activates host cellular signaling pathways, including the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, which regulates diverse cellular activities related to cell growth, survival, and apoptosis. The present study demonstrated for the first time that porcine circovirus type 2 (PCV2), a major causative agent of postweaning multisystemic wasting syndrome, which is an emerging and important swine disease, can transiently induce the PI3K/Akt pathway in cultured cells at an early step during PCV2 infection. Activation of the PI3K/Akt signal was also induced by UV-irradiated PCV2, indicating that virus replication was not required for this induction. Inhibition of PI3K activation leads to reduced virus yield, which is associated with decreased viral DNA replication and lower virus protein expression. However, inhibition of PI3K activation greatly enhanced apoptotic responses as evidenced by the cleavage of poly-ADP ribose polymerase and caspase-3 as well as DNA fragmentation using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling staining during the early stage of PCV2 infection. Furthermore, the pancaspase inhibitor zVAD.fmk alleviated the reduction in Akt phosphorylation levels by inhibiting PI3K activation, indicating that the signaling promotes cell survival and thereby favors viral replication. These results reveal that an antiapoptotic role for the PI3K/Akt pathway induced by PCV2 infection to suppress premature apoptosis for improved virus growth after infection, extending our understanding of the molecular mechanism of PCV2 infection.

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Characterization of Necrotizing Lymphadenitis Associated with Porcine Circovirus Type 2 Infection

Cited by 16 publications

References 20 publications

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Infectious agents involved in reproduction failure in swine

Activation of the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway during Porcine Circovirus Type 2 Infection Facilitates Cell Survival and Viral Replication

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