Characterization of novel insect associated peptidases for hydrolysis of food proteins

Extracellular peptidases from insect- and compost-associated microorganisms: screening and usage for wheat gluten hydrolysis

“…In addition, a compost sample (plant kitchen refuse) was used for screening. Peptidases with activity against wheat gluten were found in a recently published study [20]. In contrast to the results presented in the current study, Mika et al [20] did not isolate microorganisms with peptidase activity, but used an extract of the insects (e.g., Rhizoperta domenica; lesser grain borer).…”

“…Peptidases with activity against wheat gluten were found in a recently published study [20]. In contrast to the results presented in the current study, Mika et al [20] did not isolate microorganisms with peptidase activity, but used an extract of the insects (e.g., Rhizoperta domenica; lesser grain borer). In the study of Visôtto et al [21], proteolytic microorganisms (substrate: casein) from the gut of the velvetbean caterpillar (Anticarsia gemmatalis) were screened.…”

Prolyl-specific peptidases for applications in food protein hydrolysis

“…In insects, an efficient degradation of gluten was shown by Mika et al (2015). In gluten-containing zymograms, peptidolytic activity was observed with various cereal pests.…”

“…As substrates, oligopeptides of 3 to 33 amino acids containing a central proline have been employed (reviewed by Cunningham andO'Connor 1997, Shan et al 2004). The hydrolysis products may be detected by means of MALDI-TOF-MS (Stepniak et al 2006), ESI-MS (Mika et al 2015) or HPLC-UV (Shan et al 2004(Shan et al , 2005. Additionally, immunological tests, such as Western blots or enzyme-linked immunosorbent assays (ELISA), have been used (Stepniak et al 2006, Walter et al 2014.…”

“…The authors supposed the enzyme to be a dimer with a molecular weight of 105 kDa and determined a pH optimum of 5.6, which correlates well with its localisation in the gut of the larvae. According to Mika et al (2015), insect-associated peptidases may efficiently hydrolyse various food proteins. An extensive degradation of proline-rich proteins like casein, rice protein and gluten was shown for the cereal pests Rhizopertha dominica, Oryzaephilus surinamensis, T. molitor and Alphitobius diaperinus.…”