Characterization of Novel Selected Microalgae for Antioxidant Activity and Polyphenols, Amino Acids, and Carbohydrates

Abstract

The biochemical composition of three novel selected microalgae strains (Chlorophyta) was evaluated to confirm their potential possibilities as new sustainably produced biomass with nutritional, functional, and/or biomedical properties. Extracts from cultured Pseudopediastrum boryanum, Chloromonas cf. reticulata, and Chloroidium saccharophilum exhibited higher radical scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) when compared to butylated hydroxytoluene (BHT), but lower than butylated hydroxyanisole (BHA). Total phenolic compounds and amino acids were determined by newly developed RP-HPLC methods. Total phenolic contents, as µg g−1 of dry biomass, reached 27.1 for C. cf. reticulata, 26.4 for P. boryanum, and 55.8 for C. saccharophilum. Percentages of total analysed amino acids were 24.3, 32.1, and 18.5% of dry biomass, respectively, presenting high values for essential amino acids reaching 54.1, 72.6, and 61.2%, respectively. Glutamic acid was the most abundant free amino acid in all microalgae samples, followed by proline and lysine in C. saccharophilum and P. boryanum, and methionine and lysine in C. reticulata. Soluble carbohydrates in aqueous extracts ranged from 39.6 for C. saccharophilum to 49.3% for C. reticulata, increasing values to 45.1 for C. saccharophilum and 52.7% for P. boryanum in acid hydrolysates of dried biomass. Results confirmed the potential possibilities of these microalgae strains.

Copper toxicity leads to accumulation of free amino acids and polyphenols in Phaeodactylum tricornutum diatoms

“…The flow rate was 1 mL min −1 and the injection volume was 10 µL. The following program for eluent A was applied: 0 min, 75%; 30 min, 40%; 40 min, 40%; and finally, column was washed and reconditioned (Santiago-Díaz et al 2022 ). Data acquisition was carried out with ChromNav software, and the statistical analysis (available as supplementary data) was made using Past software provided with an ANOVA test and a Tukey’s test.…”

“…The gradient elution method for A was from 0 to 5 min, 80% isocratic; from 5 to 30 min, linear gradient from 80 to 40%; and the column was washed with a mixture of A and B (1:9) and conditioned for the next analysis. Simultaneous monitoring was set at 270 nm (GAL, PA, CAT, VA, RU, ECAT, and SA) and 324 nm (GA, CA, and FA) for quantification (Santiago-Díaz et al 2022 ). Algae samples were analyzed by triplicate, and the results were expressed as attomol (amol) cell −1 .…”

Extraction and Characterization of Bioactive Compounds from Diverse Marine Microalgae and Their Potential Antioxidant Activities

“…reticulata, and Chloroidium saccharophilum. [31] Flavonoids Flavonoids, a notable subclass of phenolic compounds, are ubiquitously present in vegetables, grains, fruits, and certain plant roots, where they serve various critical functions. These functions encompass metabolic regulation, protection of plants against the harmful effects of sunlight radiation, and defense against pathogens.…”

Bioaccessibility and bioactivities of phenolic compounds from microalgae during in vitro digestion and colonic fermentation

“…reticulata , Pseudopediastrum boryanum , and Chloroidium saccharophilum. 2,3 In addition, the abundant bioactive phenolic compounds can be used for their antioxidant, anti-inflammatory, antimicrobial and antiproliferative effects. 4…”

“…reticulata, Pseudopediastrum boryanum, and Chloroidium saccharophilum. 2,3 In addition, the abundant bioactive phenolic compounds can be used for their antioxidant, anti-inflammatory, antimicrobial and antiproliferative effects. 4 Phlorotannin, the integral structural component of some microalgal cell walls, has been shown to have secondary ecological functions, including UV radiation protection, reproductive effects and biotic defence factors.…”