Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system

Deliloğlu-Gürhan, S İsmet; Vatansever, H. Seda; Özdal-Kurt, F.; Tuğlu, Mehmet İbrahim

doi:10.1016/j.acthis.2005.11.001

Cited by 33 publications

(23 citation statements)

References 28 publications

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“…The proliferation of rBMSCs and ROB was determined by the MTT assay, which measures reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to a purple formazan product [21]. Passage 1 rBMSCs or passage 2 ROB was seeded at a density of 2 × 10 3 cells/well in 96-well plates and cultured in DMEM/F12 or α -MEM supplemented with 3% FBS and 7-methoxycoumarin or osthole at different concentrations from 10 −8 to 10 −4 M. After 48 h, culture medium was discarded and cultures were rinsed twice with deionized water, and then 50 μ L MTT solution (0.5 mg/mL) was added.…”

Section: Methodsmentioning

confidence: 99%

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

Zhai

Pan

Niu

et al. 2014

International Journal of Endocrinology

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Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects.

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Section: Methodsmentioning

confidence: 99%

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

Zhai

Pan

Niu

et al. 2014

International Journal of Endocrinology

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“…Osteoblast proliferation was measured by MTT assay (Deliloglu-Gurhan et al 2006). Briefly, osteoblasts were digested with 0.25% trypsinase, and seeded at a density of 4×10 3 cells per well in a 96-well plate.…”

Section: Osteoblast Proliferation Testmentioning

confidence: 99%

17β-estradiol combined with testosterone promotes chicken osteoblast proliferation and differentiation by accelerating the cell cycle and inhibiting apoptosis in vitro

et al. 2010

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Medullary bone is a unique tissue in the long bones cavities of lay hens, and plays an important role as a calcium reservoir for egg-shell formation. Medullary bone formation requires the synergistic action of estrogen and androgen on osteoblasts during the early stage of sexual maturity. The objective of the current study was to investigate the effects of 17beta-estradiol, testosterone, and the combination on the proliferation, alkaline phosphatase (ALP) activity, apoptosis, the cell cycle of chicken osteoblasts in vitro. The proliferation of osteoblasts was examined with the MTT assay. Apoptosis and the cell cycle were assessed with flow cytometry. Either 17beta-estradiol (200 pg ml(-1)) or testosterone (100 pg ml(-1)) or the combination (100 pg ml(-1) each) significantly enhanced osteoblast proliferation and ALP activity, accelerated the osteoblast cell cycle, and stimulated osteoblast DNA synthesis in a period of 24 h. 17beta-estradiol, used alone or with testosterone, inhibited chicken osteoblast apoptosis; However, testosterone alone induced cell apoptosis. In conclusion, 17beta-estradiol combined with testosterone promoted osteoblast proliferation and ALP activity, accelerated the osteoblast cell cycle, inhibited osteoblast apoptosis.

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“…They can be greatly expanded in vitro and induced to differentiate into osteogenic, chondrogenic, adipogenic and other mesenchymal lineages [1][2][3]. Recent reports suggest that BMSCs may also be induced to differentiate into neural cells [4].…”

Section: Introductionmentioning

confidence: 99%

GM-CSF enhances neural differentiation of bone marrow stromal cells

et al. 2007

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Recent reports suggest that bone marrow stromal cells may be induced into neural cells both in vivo and in vitro. The factors that regulate the neural differentiation and the mechanism involved, however, remains unclear. Here we demonstrated that granulocyte-macrophage colony-stimulating factor (GM-CSF), a potent hematopoietic factor, was able to enhance the neural differentiation of bone marrow stromal cells. Moreover, we found that GM-CSF receptors are abundantly distributed in the bone marrow stromal cells and GM-CSF significantly upregulated the phosphorylation of cAMP-responsive element binding protein in bone marrow stromal cells. These findings suggest that GM-CSF may activate its receptor and then enhance neural differentiation of bone marrow stromal cells by upregulating phosphorylation of cAMP-responsive element binding protein.

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Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system

Cited by 33 publications

References 28 publications

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone ResorptionIn Vitro

17β-estradiol combined with testosterone promotes chicken osteoblast proliferation and differentiation by accelerating the cell cycle and inhibiting apoptosis in vitro

GM-CSF enhances neural differentiation of bone marrow stromal cells

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