Characterization of p75+ ectomesenchymal stem cells from rat embryonic facial process tissue

Wen, Xiang; Liu, Luchuan; Deng, Min‐Zhi; Zhang, Li; Li, Rui; Xing, Yongjun; Zhou, Xia; Nie, Xin

doi:10.1016/j.bbrc.2012.08.109

Cited by 21 publications

(44 citation statements)

References 29 publications

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“…CNC‐originated EMSCs play a crucial role in tooth morphogenesis. In previous studies, we developed in vitro stem cell models to represent CNC‐originating cells and confirmed that p75NTR‐positive EMSCs from CNC demonstrated higher potential of multidifferentiation. Thus, we focused on the role of EMSCs in differential mineralization by regulating p75NTR to elucidate the underlying mechanisms in initial tooth developmental stage.…”

Section: Discussionmentioning

confidence: 54%

“…Recent studies specifically focus on tooth morphogenesis and development . p75NTR has been used as a cell surface marker to sort pure CNC‐originated EMSCs . Further study demonstrated that p75NTR‐positive EMSCs showed better mineralization and proliferation stability in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…p75NTR (P75 neurotrophin receptor) is the low‐affinity‐binding component of neurotrophin growth factor (NGF) and belongs to type I transmembrane TNF receptor superfamily . It regulates a wide range of cellular functions, including programmed cell death, cell proliferation and multidifferentiation . Recent studies specifically focus on tooth morphogenesis and development .…”

Section: Introductionmentioning

confidence: 99%

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p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

Yang

Wang

et al. 2016

Cell Proliferation

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

Yang

Wang

et al. 2016

Cell Proliferation

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“…Sox10 [98][99][100][101][102][103], we confirmed that all rNCSCs cultured under normal gravity (i.e., 2D-cultured) expressed all of these neural crest markers ( This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction. The final published version may differ from this proof.…”

Section: Decreased Expression Of Neural Crest Markers and Increased Ementioning

confidence: 57%

Simulated Microgravity Disrupts Cytoskeleton Organization and Increases Apoptosis of Rat Neural Crest Stem Cells Via UpregulatingCXCR4Expression and RhoA-ROCK1-p38 MAPK-p53 Signaling

Lin

Gou

Hsia

et al. 2016

Stem Cells and Development

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Neural crest stem cells (NCSCs) are a population of multipotent stem cells that are distributed broadly in many tissues and organs and are capable of differentiating into a variety of cell types that are dispersed throughout three germ layers. We are interested in studying the effects of simulated microgravity on the survival and self-renewal of NCSCs. NCSCs extracted from the hair follicle bulge region of the rat whisker pad were cultured in vitro, respectively, in a 2D adherent environment and a 3D suspension environment using the rotatory cell culture system (RCCS) to simulate microgravity. We found that rat NCSCs (rNCSCs) cultured in the RCCS for 24 h showed disrupted organization of filamentous actin, increased globular actin level, formation of plasma membrane blebbing and neurite-like artifact, as well as decreased levels of cortactin and vimentin. Interestingly, ∼70% of RCCS-cultured rNCSCs co-expressed cleaved (active) caspase-3 and neuronal markers microtubule-associated protein 2 (MAP2) and Tuj1 instead of NCSC markers, suggesting stress-induced formation of neurite-like artifact in rNCSCs. In addition, rNCSCs showed increased C-X-C chemokine receptor 4 (CXCR4) expression, RhoA GTPase activation, Rho-associated kinase 1 (ROCK1) and p38 mitogen-activated protein kinase (MAPK) phosphorylation, and p53 expression in the nucleus. Incubation of rNCSCs with the Gα protein inhibitor pertussis toxin or CXCR4 siRNA during RCCS-culturing prevented cytoskeleton disorganization and plasma membrane blebbing, and it suppressed apoptosis of rNCSCs. Taken together, we demonstrate for the first time that simulated microgravity disrupts cytoskeleton organization and increases apoptosis of rNCSCs via upregulating CXCR4 expression and the RhoA-ROCK1-p38 MAPK-p53 signaling pathway.

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“…The ectomesenchymal part of cranial neural crest can form the typical derivatives of mesenchymal stem cells such as connective tissue, cartilage, bone and dentine during development [Wen et al, 2012]. However, as part of the neural crest, EMSCs have a strong tendency to differentiate into neurons [Delorme et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Liu

Chen

Zhang

et al. 2013

Cells Tissues Organs

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Fibrin has been widely used in wound healing. However, its benefit for spinal cord injury (SCI) is limited. In this study, we investigated the impact of fibrin scaffolds containing ectomesenchymal stem cells (EMSCs) on histological and behavioral recovery after SCI and compared it with fibrin alone. To achieve this, EMSCs derived from adult rat nasal respiratory mucosa were cultured, characterized and transfected with green fluorescent protein adenovirus before transplantation. Then, Sprague-Dawley host rats were randomly assigned into four groups: the control group (laminectomy); the SCI group (laminectomy and transection of spinal cords); the fibrin group (fibrin was transplanted immediately after SCI), and the fibrin cell (FC) group (fibrin scaffolds containing EMSCs were transplanted after SCI). Three days after the operation, a TUNEL assay indicated less apoptotic cells in the FC group than in the fibrin group. Two weeks after SCI, fluorescence staining demonstrated not only the survival and migration of EMSCs into the lesion sites, but also a higher number of nerve fibers in the FC group than in the fibrin group. Histological examination including immunohistochemistry and transmission electron microscopy 12 weeks after the operation showed more nerve fibers and a thicker myelin sheath in the FC group compared to the fibrin group. Western blotting confirmed these morphological results. Consistent with the histological results, Basso, Beattie and Bresnahan locomotor scores of the FC group were higher than those of the fibrin group. These results suggest that fibrin scaffolds containing EMSCs can improve the behavioral and histological recovery after SCI better than fibrin alone.

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Characterization of p75+ ectomesenchymal stem cells from rat embryonic facial process tissue

Cited by 21 publications

References 29 publications

p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

Simulated Microgravity Disrupts Cytoskeleton Organization and Increases Apoptosis of Rat Neural Crest Stem Cells Via UpregulatingCXCR4Expression and RhoA-ROCK1-p38 MAPK-p53 Signaling

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

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