Characterization of Pasteurella multocida involved in rabbit infections

Massacci, Francesca Romana; Magistrali, Chiara Francesca; Cucco, Lucilla; Curcio, Ludovica; Bano, Luca; Mangili, Piermario; Scoccia, Eleonora; Bisgaard, Magne; Aalbæk, Bent; Christensen, Henrik

doi:10.1016/j.vetmic.2017.11.023

Cited by 56 publications

(51 citation statements)

References 34 publications

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“…The 205 P. multocida isolates in this study were grouped into 4 serotypes of A:L6, A:L3, D:L6 and D:L3. In consistence with the previous reports, the isolates of capsular type A (76.10%) or LPS genotype L3 (56.10%) were the most predominate strains [6][7][8][9][10]. However, the highly pathogenic strain of capsular type F was not detected in this study [6,7,10,13].…”

Section: P Multocida Strains Were Classified Into 5 Capsular Types (supporting

confidence: 86%

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

Wang¹,

Sang

Sun

et al. 2019

Preprint

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Background: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. Results: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80%, 15.61%, 9.27% and 2.44%, respectively. Conclusions: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

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Section: P Multocida Strains Were Classified Into 5 Capsular Types (supporting

confidence: 86%

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

Wang¹,

Sang

Sun

et al. 2019

Preprint

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“…In vitro analyses for antimicrobial activity of HI and TM fat Bacterial strains were selected considering their impact on rabbit and on public health [32][33][34][35]: Salmonella tiphymurium (CIP 60.62T), Salmonella enteritidis (CRBIP 19.329), Yersinia enterocolitica (CIP 101.776), Pasteurella multocida (CIP 100.610) and Listeria monocytogenes (CIP 82.110T); all these were purchased from Institute Pasteur (Paris, France). After overnight incubation following producers' instructions, strains were mixed with TM and HI fats [12] in order to reach a nal concentration of 3 Log CFU/mL, veri ed by immediate plate streaks.…”

Section: Animal Ethics Statementmentioning

confidence: 99%

Antimicrobial effects of Black Soldier Fly and Yellow Mealworm fats and their impact on gut microbiota of growing rabbits

Dabbou

Ferrocino

Gasco

et al. 2020

Preprint

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Background The study aimed to evaluate the in vitro antimicrobial activities of two types of insect fats extracted from black soldier fly larvae (HI, Hermetia illucens L.) and yellow mealworm larvae (TM, Tenebrio molitor L.) and their effects as dietary replacement of soybean oil (S) on caecal fermentation pattern, and faecal and caecal microbiota in rabbits. Results A total of 120 weaned rabbits were randomly allotted to 3 dietary treatments (40 rabbits/group): a control diet (C diet) containing 1.5% of S and two experimental diets (HID and TMD) where S was totally substituted by HI or TM fats. Regarding the in vitro antimicrobial activities, HI and TM fats did not show any effects on Salmonella growth. Yersinia enterocolitica showed lower growth when challenged with insect fats than the controls. The insect fat supplementation in rabbit diets increased the contents of the caecal volatile fatty acids when compared to the control group. A metataxonomic approach was adopted to investigate the shift in the microbial composition as a function of the dietary insect fat supplementation. The microbiota did not show a clear separation as a function of the inclusion, even if a specific microbial signature was observed. Indeed, HI and TM fat supplementation enriched the presence of Akkermansia that was found to be correlated with NH3-N concentration. An increase in Ruminococcus, which can improve the immune response of the host, was also observed. Conclusions This study confirms the potential of HI and TM fats as antibacterial feed ingredients with a positive influence on the rabbit caecal microbiota, thus supporting the possibility of including HI and TM fats in rabbit diets.

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“…Rabbits are common hosts for P. multocida. The P. multocida infected rabbits are mainly characterized by rhinitis, tracheitis, pneumonia and otitis media [6][7][8]. To our knowledge, pasteurellosis was common and widespread in rabbits in Fujian Province, whereas the knowledge about the epidemiology and characterization of P. multocida was limited.…”

Section: Introductionmentioning

confidence: 92%

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

Wang

Sang

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Pasteurella multocida is one of the important pathogens infects rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined capsular serogroups, lipopolysaccharide (LPS) genotypes and multi-locus sequence types, screened virulence factors, and tested antimicrobial susceptibility. Results The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205), and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, the virulence genes of ptfA, fur, hgbB, ompA, ompH and oma87 were positive, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80%, 15.61%, 9.27% and 2.44%, respectively. Conclusions This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.

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Characterization of Pasteurella multocida involved in rabbit infections

Cited by 56 publications

References 34 publications

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

Antimicrobial effects of Black Soldier Fly and Yellow Mealworm fats and their impact on gut microbiota of growing rabbits

Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

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