Characterization of Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP16 Prospective for Biocontrol of Potato Soft Rot

Воронина, М. В.; Bugaeva, Eugenia N; Vasiliev, Dmitry M; Kabanova, Anastasia P.; Баранник, А. П.; Shneider, Mikhail M.; Kulikov, Eugene E.; Korzhenkov, Aleksei A.; Toschakov, Stepan V; Игнатов, А. Н.; Miroshnikov, Konstantin A.

doi:10.1134/s0026261719040118

Cited by 23 publications

(16 citation statements)

References 28 publications

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“…Phages naturally occur in the environment and are easily biodegradable, which make them suitable for organic agriculture (Frampton et al, 2012;Buttimer et al, 2017;Svircev et al, 2018). Successful applications of phages to protect potatoes from SRP are reported in simulated conditions (Czajkowski et al, 2017;Carstens et al, 2019) and field trials (Adriaenssens et al, 2012;Voronina et al, 2019a). However, these experiments employed a limited number of phages and/or model strains of pathogen initially known to be susceptible to the phage used.…”

Section: Discussionmentioning

confidence: 99%

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

et al. 2020

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Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy:The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.

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Section: Discussionmentioning

confidence: 99%

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

et al. 2020

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“…There has been, however, an extensive amount of research on biocontrol of the most widespread economically important SRP, especially Dickeya solani , P. carotovorum and P. atrosepticum [ 5 , 134 , 135 , 136 , 137 , 138 , 139 , 140 ]. In fact, biocontrol of SRP using bacteriophages has progressed the most among other methods, with a stage where formulated commercial product is available, including Biolyse ® , which was demonstrated to be efficacious at preventing soft rot in packaged potatoes caused by Pectobacterium and Dickeya species [ 141 ].…”

Section: Management and The Control Of Pbrmentioning

confidence: 99%

“…Pectobacterium brasiliense (Pbr) is a worldwide-distributed bacterial plant pathogen causing soft rot of a wide range of economically important crops. Pbr has been reported in Eurasia: from Western European countries to Russia and China [ 4 , 5 , 6 ], in Africa: South Africa, Morocco, Algeria and Kenya [ 7 , 8 , 9 , 10 ]. Pbr has been constantly reported since 2004 causing significant losses particularly of potato ( Solanum tuberosum L.) [ 11 , 12 , 13 , 14 ] and posing a threat to worldwide potato gross production value which is estimated to be $63914 million in 2018 [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

et al. 2021

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Pectobacterium brasiliense (Pbr) is considered as one of the most virulent species among the Pectobacteriaceae. This species has a broad host range within horticulture crops and is well distributed elsewhere. It has been found to be pathogenic not only in the field causing blackleg and soft rot of potato, but it is also transmitted via storage causing soft rot of other vegetables. Genomic analysis and other cost-effective molecular detection methods such as a quantitative polymerase chain reaction (qPCR) are essential to investigate the ecology and pathogenesis of the Pbr. The lack of fast, field deployable point-of-care testing (POCT) methods, specific control strategies and current limited genomic knowledge make management of this species difficult. Thus far, no comprehensive review exists about Pbr, however there is an intense need to research the biology, detection, pathogenicity and management of Pbr, not only because of its fast distribution across Europe and other countries but also due to its increased survival to various climatic conditions. This review outlines the information available in peer-reviewed literature regarding host range, detection methods, genomics, geographical distribution, nomenclature and taxonomical evolution along with some of the possible management and control strategies. In summary, the conclusions and a further directions highlight the management of this species.

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“…In recent years, bacteriophages specifically infecting and killing bacteria have emerged as a promising approach for control of bacterial plant diseases [ 31 ]. Several bacteriophages to potentially control soft-rot disease caused by P. carotovorum in several important plants have been reported, such as My1 [ 32 ], PP1 [ 33 ], PM2 [ 34 ], PPWS1 [ 35 ], PP2 [ 36 ], PP16 [ 37 ], and POP72 [ 38 ]. However, no bacteriophages were isolated or demonstrated to effectively control soft-rot disease caused by P. odoriferum .…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Soft Rot Caused by Pectobacterium odoriferum with Bacteriophage phiPccP-1 in Kimchi Cabbage

Lee

et al. 2021

Microorganisms

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Pectobacterium odoriferum has recently emerged as a widely infective and destructive pathogen causing soft-rot disease in various vegetables. Bacteriophage phiPccP-1 isolated from Pyeongchang, South Korea, showed lytic activity against P. odoriferum Pco14 and two other Pectobacterium species. The transmission electron microscopy and genome phylograms revealed that phiPccP-1 belongs to the Unyawovirus genus, Studiervirinae subfamily of the Autographivirinae family. Genome comparison showed that its 40,487 bp double-stranded DNA genome shares significant similarity with Pectobacterium phage DU_PP_II with the identity reaching 98% of the genome. The phiPccP-1 application significantly inhibited the development of soft-rot disease in the mature leaves of the harvested Kimchi cabbage up to 48 h after Pco14 inoculation compared to the untreated leaves, suggesting that phiPccP-1 can protect Kimchi cabbage from soft-rot disease after harvest. Remarkably, bioassays with phiPccP-1 in Kimchi cabbage seedlings grown in the growth chamber successfully demonstrated its prophylactic and therapeutic potential in the control of bacterial soft-rot disease in Kimchi cabbage. These results indicate that bacteriophage phiPccP-1 can be used as a potential biological agent for controlling soft rot disease in Kimchi cabbage.

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Characterization of Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP16 Prospective for Biocontrol of Potato Soft Rot

Cited by 23 publications

References 28 publications

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection

Pectobacterium brasiliense: Genomics, Host Range and Disease Management

Biocontrol of Soft Rot Caused by Pectobacterium odoriferum with Bacteriophage phiPccP-1 in Kimchi Cabbage

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