Characterization of plasma proteome in biliary atresia

Lee, Chi Wei; Meng, Lin; Lee, Wen‐Chien; Chou, Ming Huei; Hsieh, Chih Sung; Lee, Shin-Ye; Chuang, Jiin Haur

doi:10.1016/j.cca.2006.06.019

Cited by 8 publications

(7 citation statements)

References 13 publications

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“…In the present study, Apo C-II was found to be downregulated in serum from patients with BA compared with healthy infants, which is consistent with previously reported data (18); however, Apo C-II was upregulated in serum from patients with BA compared with patients with non-BA NC. Consistent with our data, previous reports demonstrated that Apo C-II expression was elevated in individuals with primary biliary cirrhosis and BA compared with non-BA NC (17,25).…”

Section: Discussionsupporting

confidence: 93%

“…SELDI-TOF-MS analysis has been successfully used to identify specific biomarkers of pediatric surgery diseases, including nephroblastoma and Hirschsprung disease (13)(14)(15), and to determine genetic risk in preimplantation genetic diagnosis using maternal plasma (16). A few pilot studies have reported the potential of 2-dimensional difference gel electrophoresis (2-DE) to identify serum biomarkers that differentiate BA from other types of neonatal cholestasis and healthy subjects (17,18); however, no specific protein biomarkers were identified and validated in these reports. Additionally, proteome coverage by 2-DE is experimentally limited to proteins with molecular weights in the 10-to 120-kDa range.…”

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confidence: 99%

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Identification of Serum Protein Biomarkers in Biliary Atresia by Mass Spectrometry and Enzyme‐linked Immunosorbent Assay

Song

Dong

Fan

et al. 2012

J. pediatr. gastroenterol. nutr.

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Section: Discussionsupporting

confidence: 93%

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confidence: 99%

Identification of Serum Protein Biomarkers in Biliary Atresia by Mass Spectrometry and Enzyme‐linked Immunosorbent Assay

Song

Dong

Fan

et al. 2012

J. pediatr. gastroenterol. nutr.

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“…Before discussing the potential significance of proteins in our classifier, we note that a recent analysis demonstrated changes in serum apolipoprotein C-II and transthyretin in infants with BA at early and late stages of disease (12). Although these proteins were among our diagnostic biomarkers, comparing their data with ours is difficult because of the few control samples in their study (n ¼ 2) and their emphasis on changes in serum proteins with disease progression in BA versus our emphasis on differential diagnosis between BA and other cholestatic disease.…”

Section: Discussionmentioning

confidence: 99%

Serum Markers May Distinguish Biliary Atresia From Other Forms of Neonatal Cholestasis

Wang¹,

Malone

Gilmore

et al. 2010

J. pediatr. gastroenterol. nutr.

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Background Biliary atresia (BA) is the most serious liver disease in infants. Diagnosis currently depends on surgical exploration of the biliary tree. Non-invasive tests that distinguish BA from other types of neonatal liver disease are not available. Methods To identify potential serum biomarkers that classify children with neonatal cholestasis, we performed 2-dimensional difference gel electrophoresis, statistical analysis, and tandem mass spectrometry using serum samples from 19 infants with BA and 19 infants with non-BA neonatal cholestasis. Results 11 potential serum biomarkers were found that could in combination classify children with neonatal cholestasis. Conclusions Although no single biomarker or imaging test adequately distinguishes BA from other types of neonatal cholestasis, combinations of biomarkers, imaging tests and non-invasive clinical criteria should be further explored as potential tests for rapid and accurate diagnosis of BA.

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“…The spots with different expression were selected as described previously, digested and used for MS analysis. Since many articles used the criteria of more than 1.5-fold change, we used this criteria [7][8][9]. The results of LC-MS/MS for the spots are shown in Table 1.…”

Section: Detection and Identification Of Spots With Altered Expressiomentioning

confidence: 99%

Downregulation of two isoforms of ubiquitin carboxyl‐terminal hydrolase isozyme L1 correlates with high metastatic potentials of human SN12C renal cell carcinoma cell clones

Tanaka¹,

Kuramitsu²,

Fujimoto³

et al. 2008

Electrophoresis

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Proteomic differential display analysis was performed on human renal cell carcinoma cell SN12C clones having different metastatic potentials by using 2-DE and LC-MS/MS. The SN12C cell clones were SN12C parent cell line, SN12C-clone 2, SN12C-clone 4, and SN12C-PM6. The SN12C parent cell line was established from an HRCC surgical specimen. SN12C-clone 4 has lower, and SN12C-clone 2 and SN12C-PM6 have higher metastatic potential than SN12C parent cells. We found eight protein spots whose expression level was different between low metastatic clones and high metastatic clones. The protein expression of three appeared to be higher in high metastatic clones than low metastatic clones, and that of other five protein spots appeared to be lower in high metastatic clones than low metastatic clones. These spots were selected, digested and analyzed by LC-MS/MS analysis, and they were identified by peptide sequencing tag. In high metastatic potential clones, two isoforms of ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) were downregulated. These results suggest that UCH-L1 expression seems to be associated with the metastatic potential of HRCC SN12C cell clones.

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Characterization of plasma proteome in biliary atresia

Cited by 8 publications

References 13 publications

Identification of Serum Protein Biomarkers in Biliary Atresia by Mass Spectrometry and Enzyme‐linked Immunosorbent Assay

Identification of Serum Protein Biomarkers in Biliary Atresia by Mass Spectrometry and Enzyme‐linked Immunosorbent Assay

Serum Markers May Distinguish Biliary Atresia From Other Forms of Neonatal Cholestasis

Downregulation of two isoforms of ubiquitin carboxyl‐terminal hydrolase isozyme L1 correlates with high metastatic potentials of human SN12C renal cell carcinoma cell clones

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