Characterization of poly(vinylferrocenium) coated surfaces and their applications in DNA sensor technology

Abstract

Poly(vinylferrocenium) (PVF ? ) modified gold (Au) electrode was developed in this study for the electrochemical sensing of deoxyribonucleic acid (DNA) hybridization based on the oxidation signals of polymer and guanine, and also for the electrochemical investigation of interaction of anticancer drug, mitomycin C (MC) and DNA immobilized onto PVF ? modified Au electrode. PVF ? modified Au electrode was prepared by electrooxidation of poly(vinylferrocene) PVF at ?0.7 V versus Ag/AgCl reference electrode. The polymer modified electrode and DNA immobilized polymer modified electrode were characterized by X-ray photoelectron (XPS), Fourier transform infrared-attenuated total reflentance (FTIR-ATR) and alternating current (AC) impedance spectroscopy. For application studies, differential pulse voltammetry (DPV) technique was used.

Interaction of Mitomycin C with DNA Immobilized onto Single‐walled Carbon Nanotube/Polymer Modified Pencil Graphite Electrode

“…The detection limit corresponded to 625 ng/mL ( S / N =3) for MC using ctdsDNA immobilized SWCNT/ PVF + modified electrode 43. This value was comparable to the ones obtained by different types of electrodes reported earlier in the literature 27, 44.…”

Graphene oxide integrated sensor for electrochemical monitoring of mitomycin C–DNA interaction

“…5E and F, a to a 0 and b to b 0 ). A similar significant decrease of the guanine signal was explained in earlier reports [38][39][40]44 by the shielding of oxidizable groups of electroactive DNA bases following the surface-confined interaction of the drug within dsDNA.…”

“…41,44,51 With respect to the electrode modification approach, a simpler and faster modification step was used herein for the development of disposable GO-modified electrodes by following passive adsorption. In addition, a smaller amount of nucleic acid was employed compared to the electrodes modified with poly(vinylferrocenium) (PVF + ), 40 or single-walled carbon nanotube (SWCNT)/PVF + , 52 prepared electrochemically by potentialcontrolled coulometry technique.…”

“…36,37 Earlier studies were focused on monitoring the MC-DNA interaction process by using different electrochemical transducers such as the hanging mercury drop electrode (HMDE), carbon screen-printed electrodes (CSPEs) and carbon paste electrodes (CPEs). [38][39][40][41] Perez et al investigated the electrochemical detection of DNA-MC adducts at the hanging mercury drop electrode (HMDE) through their potential controlled interaction at the electrode surface. 41 Karadeniz et al investigated the interaction process in a microemulsion system carrying the anticancer drug MC and DNA by using voltammetric techniques.…”

Design of a probe based on poly(glycidyl methacrylate‐co‐vinylferrocene)‐coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues

“…The detection of DNA based on the electrochemical behavior of the supporting material arising from redox couple has been successfully applied to the process of the immobilized electrodes . Additionally, another method used for the DNA detection is based on monitoring the change in the current response of the electroactive materials such as Co(bpy) 3 3+ and Fe(CN) 6 3−/4‐ with the hybridization of the target DNA squence to the DNA probe.…”