Characterization of polymorphic microsatellite markers for Microcyclus ulei, causal agent of South American leaf blight of rubber trees

Abstract

South American leaf blight caused by the ascomycete Microcyclus ulei is the most harmful disease of the rubber tree in Latin America and a potential threat to Asiatic and African natural rubber production. Until now, the variability of this fungus was assessed through observation of pathogenicity of isolates on a range of rubber tree clones with known resistance reactions. The present study describes the process used to design 11 microsatellite markers and evaluates their usefulness in detecting genetic polymorphism. Nine of these markers were polymorphic among six isolates from Brazil (with two to three alleles per locus) and five markers were polymorphic among four isolates from French Guiana (with two to four alleles per locus).

Understanding the recent colonization history of a plant pathogenic fungus using population genetic tools and Approximate Bayesian Computation

“…Out of 32 loci tested, 16 were found to be polymorphic with two to eight alleles (Supplementary Table S1). Seven of these polymorphic markers were identical to the previously described microsatellites (Le Guen et al, 2004), and among these, most (Mu01, Mu02, Mu07, Mu08 and Mu12) have different primer sequences in order to allow size multiplexing. Furthermore, identical annealing temperatures were chosen for all primer pairs in order to allow multiplexed PCRs.…”

“…Nine polymorphic microsatellite markers had already been developed for M. ulei (Le Guen et al, 2004). In order to increase the power of the population genetics analysis, supplementary microsatellite markers were designed (Supplementary Table S1).…”

“…In order to increase the power of the population genetics analysis, supplementary microsatellite markers were designed (Supplementary Table S1). New DNA sequences were obtained from the microsatellite-enriched library used by Le Guen et al (2004). Forty-two primers pairs were defined using PRIMER3 (Rozen and Skaletsky 2000).…”

Identification and comparison of natural rubber from two Lactuca species

“…However, latex allergies triggered by H. brasiliensis proteins affect >7% of the population and can be very severe (Rolland et al, 2005). In addition, the H. brasilinesis crop consists predominately of plantation-grown clonal trees that lack genetic diversity, thereby rendering the crop vulnerable to pathogenic attack (Le Guen et al, 2004). For these reasons, as well as political and economic instability in some countries producing and exporting H. brasiliensis rubber, there is considerable interest in finding alternative commercial sources of natural rubber in crop plants that grow well in northern climates.…”

SUITABILITY OF INTERGENIC SPACER OR INTERNAL TRANSCRIBED SPACER MICROSATELLITE‐PRIMED PCR FOR THE IDENTIFICATION OF RHIZOCTONIA SOLANI AND SOME PHYTOFUNGI

“…The discriminatory power of some methods appears insufficient when these methods are tested alone. These techniques include random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLPs) and microsatellites or simple sequence repeats (SSRs) (Le Guen et al. 2004; Rubini et al.…”