Characterization of polymorphism in the FSH receptor gene and its impact on some reproductive indices in dairy cows

Sharifiyazdi, Hassan; Mirzaei, Abdolah; Ghanaatian, Zahra

doi:10.1016/j.anireprosci.2017.11.006

Cited by 19 publications

(25 citation statements)

References 25 publications

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“…Another gene -FSHB, coding beta subunit of follicle-stimulating hormone, was detected in the second region on BTA15. Several studies proved a significant effect of this gene on reproduction quality in cattle (Sharifiyazdi et al 2018).…”

Section: Analysis Of Selection Signatures In the Beef Genomementioning

confidence: 97%

Analysis of selection signatures in the beef cattle genome

Moravčíková¹,

Kasarda²,

Vostrý³

et al. 2019

Czech J. Anim. Sci.

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This study aimed to evaluate the impact of selection on the genome structure of beef cattle through identification of selection signatures reflecting the breeding standard of each breed and to discover potential functional genetic variants to improve performance traits. Genotyping data of six beef breeds (Aberdeen Angus, Hereford, Limousin, Charolais, Piedmontese and Romagnola) were used to perform genome-wide scans for selection signatures. The approaches applied were based on an assumption that selection leads to linkage disequilibrium or to a decrease of genetic variability in genomic regions containing genotypes connected with favourable phenotypes. Thus, the selection signatures were analysed based on Wright’s F<sub>ST</sub> index, distribution of runs of homozygosity segments in the beef genome and determination of linkage disequilibrium variability between breeds. The number and length of detected selection signals were different depending on the breeds and methodological approaches. As expected due to the breeding goals of analysed breeds, common signals were located on autosomes 2, 6, 7, 13 and 20 close to the genes associated with coat colour (KIT, KDR), muscle development (GDF9, GHRH, GHR), double muscling (MSTN), meat tenderness (CAST) and intramuscular fat content (SCD). But, across the genomes of analysed breeds, unique selection signals were found as well. The subsequent analysis of those single nucleotide polymorphism markers can be beneficial for the genetic progress of studied breeds in future.

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Section: Analysis Of Selection Signatures In the Beef Genomementioning

confidence: 97%

Analysis of selection signatures in the beef cattle genome

Moravčíková¹,

Kasarda²,

Vostrý³

et al. 2019

Czech J. Anim. Sci.

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“…After using PRC-RFLP, the FSHR gene segment amplified the genotypes for two Alul cutting sites (GG on one band: 243bp; CC at 239 bp and 188 bp). The FSHR gene fragment with the AluI enzyme cutting site indicates the absence of mutation [16]. The success rate of the FSHR gene amplification in this study was 100%.…”

Section: Discussionmentioning

confidence: 54%

Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

2020

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Background and Aim: The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism. Materials and Methods: Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. Results: Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188. Conclusion: Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes.

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“…Functional studies demonstrated in vitro that the nonsense mutation c.175C > T caused the loss of full‐length FSHR expression and that the p.R59X mutant showed no response to FSH stimulation of cAMP levels in a Chinese woman with primary ovarian insufficiency (Liu et al, ). The A to G mutation within the upstream region of the FSHR gene (position − 278) may affect some reproductive variables in Holstein dairy cows (Sharifiyazdi, Mirzaei, & Ghanaatian, ). The Tyr76X mutation of the FSH β‐subunit is associated with a partial phenotype of FSH deficiency in girls (Berger et al, ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

Lü

et al. 2019

Veterinary Medicine & Sci

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This study investigated the variations of the nucleotide sequences and ovarian expression levels of genes related to follicular development and atresia in prolific Jintang black goats and nonprolific Tibetan goats. Eight genes, FSHB, LHB, FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX, were examined using reverse transcription‐polymerase chain reaction and quantitative real‐time PCR. The results showed that the nucleotide and deduced amino acid sequences of the LHB and BAX genes were not different, but there was one base change in the FSHR genes between the two breeds. There was one base change in the FSHB gene, which resulted in one amino acid substitution; there were nine base changes in the LHCGR gene, which resulted in five amino acid substitutions; and there were six base changes in the B4GANT2 gene, which resulted in four amino acid substitutions. The expression levels of the FSHR, LHCGR, ESR2, B4GANT2, BCL2 and BAX genes in the ovaries were not different between the two breeds. The plasma concentrations of FSH were not different, but the plasma concentrations of LH, P4 and E2 were lower in prolific Jintang black goats than in nonprolific Tibetan goats (P ˂ 0.05) at 40 hr after removal of the Controlled Internal Drug Release Devices. These results provide some foundations elucidating the endocrine and molecular mechanisms controlling ovulation rate in goats, but these need to be further verified.

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Characterization of polymorphism in the FSH receptor gene and its impact on some reproductive indices in dairy cows

Cited by 19 publications

References 25 publications

Analysis of selection signatures in the beef cattle genome

Analysis of selection signatures in the beef cattle genome

Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

Comparison of the sequences and expression levels of genes related to follicular development and atresia between prolific and nonprolific goat breeds

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